InDel Detection of LEPR Gene and Its Association Analysis with Tail Fat and Growth Traits of Tong Sheep (Ovis aries)
YI Xiao-Hua, LIU Shi-Rong, ZHANG Yu1, PAN Yun1, YUAN Ting-Ting1, SUN Xiu-Zhu1,2,*
1 College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China; 2 College of Grassland Agriculture, Northwest A&F University, Yangling 712100, China
Abstract：Tong sheep is a well-known local sheep (Ovis aries) with fat tail and semi-fine wool for both meat and wool. Adipose tissue is an organ that stores energy and supplies energy to the body. Adipose tissue could secrete leptin (LEP), leptin receptor (LEPR), inflammatory factors and so on.. The LEP and LEPR genes in the adipocytokine signaling pathway can coordinately regulate fat deposition and energy metabolism. In this study, in order to verify the insertion/deletion (InDels) variation of the LEPR gene and its influence on the fat traits and growth traits of the sheep tail, InDel locus of the LEPR gene was screened through the Ensemble database, and primers were designed according to the sequence of Indels locus screened in the database. 166 healthy individuals of Tong sheep were identified and analyzed. Association analysis showed that the 17 bp InDel polymorphism of LEPR gene had no significant effect on tail width and tail length traits of the same sheep (P>0.05), indicating that this locus had little effect on tail fat traits; The breast depth of DD genotype at this locus was significantly higher than that of ID genotype (P>0.05). The DD genotype was significantly higher than that of the II genotype in the growth traits of ram body height, back height and recommended height (P<0.05). The ewe waist width of the II genotype at InDel locus was the significantly higher than that of the DD genotype (P<0.05), indicating that this locus had a significant impact on some growth traits. These results provide a theoretical basis for the selection of the LEPR gene in tail type of Tong sheep and the selection and breeding of the Tong sheep, and provide an important reference for the genetic breeding and improvement of the Tong sheep.
易晓华, 刘世荣, 张宇, 潘赟, 袁婷婷, 孙秀柱. LEPR基因InDel检测及其与同羊尾脂和生长性状关联分析[J]. 农业生物技术学报, 2021, 29(6): 1142-1149.
YI Xiao-Hua, LIU Shi-Rong, ZHANG Yu, PAN Yun, YUAN Ting-Ting, SUN Xiu-Zhu. InDel Detection of LEPR Gene and Its Association Analysis with Tail Fat and Growth Traits of Tong Sheep (Ovis aries). 农业生物技术学报, 2021, 29(6): 1142-1149.
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