Abstract：Major genes related to growth traits of goat (Capra hircus) were selected as marker assisted selection which can improve the accuracy of selection and accelerate breeding progress. The current study aimed to analyze gene expression characteristics of insulin-like growth factor-1 (IGF-1) gene in skeletal muscle, SNPs distribution and its association with growth traits of goats. qRT-PCR was used to analyze IGF-1 gene expression level in masseter, trapezius, semispinalis, arm triceps, extensor carpi radialis longus, longissimus dorsi, psoas, quadriceps femoris, biceps femoris of Fuqing goat and Nubia black goat. PCR sequencing and high-resolution melting (HRM) technologies were carried out to screen SNPs and analyzed their association with growth trait in Fuqing goat (n=124), Nubia black goat (n=152), Jianyang big ear goat (n=121), Daiyun goat (n=20). The results revealed that IGF-1 gene was expressed in 9 skeletal muscle tissues of Fuqing goat and Nubia black goat, and the expression level was different in skeletal muscle tissues and breeds. The expression level of IGF-1 gene in 9 skeletal muscle tissues of Fuqing goat was lower than that of Nubia black goat. The expression level of IGF-1 gene in masseter and quadriceps femoris was extremely significantly different between the 2 breeds (P<0.01), and the expression level of IGF-1 gene in arm triceps and trapezius and longissimus dorsi was significantly different between the 2 breeds (P<0.05). The exon sequence of IGF-1 gene was highly conserved, and no mutation was found in Fuqing goat and Nubia black goat. Most of the 3 SNPs of the 4 breeds showed moderate polymorphism distribution, PIC was 0.18~0.38; the 3 SNPs had obvious variety specificity on the growth traits of goats, and had significant influence on some growth traits of Jianyang big ear goat and Nubia black goat, but had little influence on the growth traits of Fuqing goat. The results of this study reveals the difference of IGF-1 gene expression in the skeletal muscles of Fuqing goat and Nubia black goat, and provides basic data for further exploring the mechanism of goat growth and development. The research results unveile that 3 SNPs within IGF-1 have strong effects on many growth traits of goats, which could be used as candidate markers for marker assisted selection of greater growth performance.
李文杨, 刘远, 吴贤锋, 黄勤楼. 山羊IGF-1基因的骨骼肌表达特性及其SNPs与生长性状的关联分析[J]. 农业生物技术学报, 2019, 27(12): 2188-2197.
LI Wen-Yang, LIU Yuan, WU Xian-Feng, HUANG Qin-Lou. Skeletal Muscle Expression Characteristics of IGF-1 Gene and Its SNPs Association with Growth Traits in Goat (Capra hircus). 农业生物技术学报, 2019, 27(12): 2188-2197.
[1] 安静, 张雪梅, 刘晨曦, 等. 2014. 绵羊IGF1基因慢病毒表达载体的构建及其促肌细胞生长作用研究[J]. 西北农林科技大学学报(自然科学版), 42(4): 15-21. (An J, Zhang X M, Liu C X, et al.2014. Construction of lentiviral vector of ovine IGF-1 gene and its effects on myoblast growth[J]. Journal of Northwest A&F University (Natural Science Edition), 42(4): 15-21.) [2] 成志敏, 张宁芳, 王媛媛, 等. 2019. 基于RNA-Seq技术筛选影响猪肌纤维性状的候选基因[J]. 畜牧兽医学报, 50(5): 918-929. (Cheng Z M, Zhang N F, Wang Y Y, et al.2019. Screening of candidate genes for muscle fiber characteristics in pig using RNA-Seq[J]. Acta Veterinaria et Zootechnica Sinica, 50(5): 918-929.) [3] 冯涛, 赵有璋, 储明星, 等. 2010. 山羊胰岛素样生长因子Ⅰ基因多态性及序列分析[J]. 安徽农业大学学报, 37(1): 26-31. (Feng T, Zhao Y Z, Chu M X, et al.2010. Polymorphism and sequence analysis on exons of IGF-1 gene in goats[J]. Journal of Anhui Argricultural University, 37(1): 26-31.) [4] 刘国庆, 黄治国, 刘振山, 等. 2006. 羔羊肝脏IGF-I和IGF-I R基因表达的发育性变化研究[J]. 中国农业科学, 39(12): 2577-2581. (Liu G Q, Huang Z G, Liu Z S, et al.2006. Studies on the developmental patterns of IGF-1 and IGF-1R gene expression in liver tissue of lamb[J]. Scientia Agricultura Sinica, 39(12): 2577-2581.) [5] 刘远, 李文杨, 吴贤锋, 等. 2019. 福清山羊与努比亚黑山羊背最长肌比较转录组分析[J]. 中国农业科学, 52(14): 2525-2537. (Liu Y, Li W Y, Wu X F, et al.2019. Transcriptome analysis of differentially gene expression associated with longissimus doris tissue in Fuqing goat and Nubian black goat[J]. Scientia Agricultura Sinica, 52(14): 2525-2537.) [6] 孙伟, 李达, 马月辉, 等. 2012. 湖羊GHR和IGF-1基因表达的发育性变化及其与肉质性状的关联[J]. 中国农业科学, 45(22): 4678-4687. (Sun W, Li D, Ma Y H, et al.2012. Developmental changes of gene expression of GHR and IGF-1 genes and their association analysis with meat quality traits in Hu sheep[J]. Scientia Agricultura Sinica, 45(22): 4678-4687.) [7] 腾藤. 2018. 白梭吻鲈IGF-1、IGF-2基因的克隆、表达及其SNP标记的开发[D]. 硕士学位论文, 苏州大学, 导师: 凌去非, pp. 5-9. (Teng T.2018. IGF-1, IGF-2 gene cloning, expression and development of their SNPs markers of Sander lucioperca[D]. Thesis for M. S., Soochow University, Supervisor: Ling Q F, pp. 5-9.) [8] 王东, 李发弟, 王维民, 等. 2015. 绵羊IGF-1和IGF-1R基因特征分析及其在肌肉组织中的表达差异[J].甘肃农业大学学报, 50(2): 6-15. (Wang D, Li F D, Wang W M, et al.2015. Bioinformatics analysis on IGF-1 and IGF-1R genes and their different expression in muscle tissue of sheep[J]. Journal of Gansu Agricultural University, 50(2): 6-15. ) [9] 王强, 朱梦婷, 郑炜, 等. 2019. 鹌鹑IGF-1、MyoD基因多态性与其肉质性状的关联分析[J]. 中国家禽, 41(1): 5-8. (Wang Q, Zhu M T, Zheng W, et al.2019. Association analysis between IGF-1 and MyoD gene polymorphisms and their meat quality traits of Quail[J]. China Poultry, 41(1): 5-8. ) [10] 徐德志, 陆新江, 鲁建飞, 等. 2019. 香鱼IGF-1基因特性及其对单核巨噬细胞德功能调控[J]. 农业生物技术学报, 27(5): 785-798. (Xu D Z, Lu X J, Lu J F, et al.2019. Gene characterization of Ayu (Plecoglossus altivelis) IGF-1 and its functional regulation on moncytes/macrophages[J]. Journal of Agricultural Biotechnology, 27(5): 785-798.) [11] 杨韩, 张阳海, 王敏, 等. 2019. 陕北白绒山羊POU1F1基因3'-UTR多态性及其与生长性状的相关分析[J]. 农业生物技术学报, 27(7): 1224-1232. (Yang H, Zhang Y H, Wang M, et al.2019. Polymorphisms of 3'-UTR of POU1F1 gene and its association with growth traits in Shaanbei White Cashmere goats[J]. Journal of Agricultural Biotechnology, 27(7): 1224-1232.) [12] 袁倩, 王柏辉, 苏琳, 等. 2019. 两种饲养方式对苏尼特羊肉脂肪酸组成和脂肪代谢相关基因表达的影响[J]. 食品科学, 40(9): 29-34. (Yuan Q, Wang B H, Su L, et al.2019. Effects of two feeding regimens on fatty acid composition and lipid metabolism-related gene expression in Sunit sheep[J]. Food Science, 40(9): 29-34. ) [13] 周瑞, 王以鑫, 龙科任, 等. 2018. LncRNA调控骨骼肌发育的分子机制及其在家养动物中的研究进展[J]. 遗传, 40(4): 292-304. (Zhou R, Wang Y X, Long K R, et al.2018. Regulatory mechanism for lncRNAs in skeletal muscle development and progress on its research in domestic animals[J]. Hereditas (Beijing), 40(4): 292-304. ) [14] 朱晓锋, 许厚强, 陈伟, 等. 2019. 从江香猪IGF-1和IGF-2基因的克隆、表达及生物信息学分析[J]. 农业生物技术学报, 27(8): 1382-1391. (Zhu X F, Xu H Q, Chen W, et al.2019. Cloning, expression and bioinformatics analysis of IGF-1 and IGF-2 genes in Congjiangxiang pigs(Sus scrofa)[J]. Journal of Agricultural Biotechnology, 27(8): 1382-1391. ) [15] Andrade P C, Grossi D A, Paz C C, et al.2008. Association of an insulin-like growth factor 1 gene microsatellite with phenotypic variation and estimated breeding values of growth traits in Canchim cattle[J]. Animal Genetics, 39(5): 480-485. [16] Bahrami A, Sh B, Miraeiashtiani S R, et al.2013. Genetic polymorphisms and protein structures in growth hormone, growth hormone receptor, ghrelin, insulin-like growth factor 1 and leptin in Mehraban sheep[J]. Gene, 527(1): 397-404. [17] Deng C, Ma R, Yue X, et al.2010. Association of IGF-I gene polymorphisms with milk yield and body size in Chinese dairy goats[J]. Genetics & Molecular Biology, 33(2): 266-270. [18] Duclos M J, Beccavin C, Simon J.1999. Genetic models for study of insulin-like growth factors (IGF) and muscle development in birds compared to mammals[J]. Domestic Animal Endocrinology, 17(2-3): 231-243. [19] Estany J, Tor M, Villalba D, et al.2007. Association of CA repeat polymorphism at intron 1 of insulin-like growth factor (IGF-I) gene with circulating IGF-I concentration, growth, and fatness in swine[J]. Physiological Genomics, 31(2): 236-243. [20] Gholibeikifard A, Aminafshar M, Mashhadi M H.2013. Polymorphism of IGF-I and ADRB3 genes and their association with growth traits in the Iranian Baluchi sheep[J]. Journal of Agricultural Science & Technology, 15(6): 1153-1162. [21] He J N, Zhang B Y, Chu M X, et al.2012. Polymorphism of insulin-like growth factor 1 gene and its association with litter size in Small Tail Han sheep[J]. Molecular Biology Reports, 39(10): 9801-9807. [22] Jones J I, Clemmons D R.1995. Insulin-like growth factors and their binding proteins: Biological actions[J]. Endocrine Reviews, 16(1): 3-34. [23] Kurdistani Z K, Rostamzadeh J, Rashidi A, et al.2013. Evaluation of insulin-like growth factor-I gene polymorphism on growth traits and yearling fleece weight in goats[J]. Small Ruminant Research, 111(1-3): 10-15. [24] Mareen N, Elisa W, Peter L, et al.2018. Characterization of igf1 and igf2 genes during maraena whitefish (Coregonus maraena) ontogeny and the effect of temperature on embryogenesis and igf expression[J]. Growth Hormone and IGF Research, 40: 32-43. [25] Mikawa S, Yoshikawa G I, Aoki H, et al.1995. Dynamic aspects in the expression of the goat insulin-like growth factor-I (IGF-I) gene: Diversity in transcription and post-transcription[J]. Bioscience, Biotechnology and Biochemistry, 59(1): 87-92. [26] Naicy T, Venkatachalapathy T, Aravindakshan T, et al.2017. cDNA cloning, structural analysis, SNP detection and tissue expression profile of the IGF1 gene in Malabari and Attappady Black goats of India[J]. Journal of Genetics, 96(2): 307-312. [27] Othman E O, Mohamed A S, Nadia E M.2016. Evaluation of insulin-like growth factor-I gene polymorphism in Egyptian small ruminant breeds[J]. Africa Journal of Biotechnology, 15(48): 2714-2719. [28] Sharma A, Dutt G, Sivalingam J, et al.2013. Novel SNPs in IGF1, GHR and IGFBP-3 genes reveal significant association with growth traits in Indian goat breeds[J]. Small Ruminant Research, 115(1-3): 7-14. [29] Thomas N, Venkatachalapathy T, Aravindakshan T, et al.2016. Molecular cloning, SNP detection and association analysis of 5' flanking region of the goat IGF1 gene with prolificacy[J]. Animal Reproduction Science, 167(4): 8-15. [30] Wang P Q, Tan Y, Zhang B Y, et al.2011. DNA polymorphisms of 5'-flanking region of insulin-like growth factor 1 gene and their association with reproduction traits in goats[J]. Journal of Integrative Agriculture, 10(10): 1609-1617. [31] Yakar S, Rosen C J, Beamer W G, et al.2002. Circulating levels of IGF-I directly regulate bone growth and density[J]. Journal of Clinical Investigation, 110(6): 771-781. [32] Yilmaz A, Davis M E, Hines H C, et al.2005. Detection of two nucleotide substitutions and putative promoters in the 5' flanking region of the ovine IGF-I gene[J]. Journal of Applied Genetics, 46(3): 307-309. [33] Zhang C X, Zhang W, Luo H L, et al.2008. A New Single nucleotide polymorphism in the IGF-I gene and its association with growth traits in the Nanjiang Huang goat[J]. Asian Australasian Journal of Animal Sciences, 21(8): 1073-1079.
