Correlation Analysis of Polymorphisms in Promoter Region and Coding Region of GHR and IGF-Ⅰ Genes with Growth Traits of Two Varieties of Nile Tilapia (Oreochromis niloticus)
CHEN Bing-Lin, XIAO Wei, ZOU Zhi-Ying, ZHU Jing-Lin, LI Da-Yu, YU Jie, YANG Hong*
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization,Ministry of Agriculture; Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi 214081,China
Abstract：The GH-IGF signal pathway plays an important role in regulating the growth of fish. In order to search for molecular markers distribution of single nucleotide polymorphisms (SNPs) of growth hormone receptor (GHR) and insulin-like growth factor-Ⅰ (IGF-Ⅰ) genes in fast-growing traits of tilapia (Oreochromis niloticus),The SNPs locus for the above genes of Nile tilapia (O. niloticus) and genetic improvement of farmed (GIFT) tilapia (O. niloticus) were screened. In this experiment,32 SNPs were screened from the promoter region and coding region of 2 tilapia GHR1,GHR2 and IGF-Ⅰ genes by Sanger sequencing technology. After functional secondary screening,a total of 9 SNPs were retained in Jifu tilapia (JF) and distributed in the GHR1 gene (7) and GHR2 gene (2),and a total of 5 SNPs were retained in Nile tilapia egyptian strain (AJ),which were distributed in GHR1 gene (4) and GHR2 gene (1). General linear model (GLM) and least significant difference (LSD) were used to analyze the correlation between SNPs and growth traits in the offspring of 2 varieties of tilapia. the results showed that 12 genotypes of 6 SNPs loci in the GHR1 and GHR2 genes of the JF population were significantly associated with fast-growing traits,while no SNPs loci and genotypes significantly associated with the fast-growing traits were found in the AJ population. The above results can be used as candidate molecular markers in molecular assisted breeding of tilapia,and provide a theoretical basis for subsequent research on growth factors in fish.
陈炳霖, 肖炜, 邹芝英, 祝璟琳, 李大宇, 喻杰, 杨弘. 两种尼罗罗非鱼GHR 、IGF-Ⅰ 基因启动子区及编码区多态性与生长性状的相关性分析[J]. 农业生物技术学报, 2020, 28(11): 2032-2047.
CHEN Bing-Lin, XIAO Wei, ZOU Zhi-Ying, ZHU Jing-Lin, LI Da-Yu, YU Jie, YANG Hong. Correlation Analysis of Polymorphisms in Promoter Region and Coding Region of GHR and IGF-Ⅰ Genes with Growth Traits of Two Varieties of Nile Tilapia (Oreochromis niloticus). 农业生物技术学报, 2020, 28(11): 2032-2047.
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