Effects of Dietary β-carotene Levels on Gene Expression of BCMO1, BCDO2 and Related Metabolic Regulators in Yak (Bos grunniens) Intestine and Correlation Analysis
REN Xiao-Ying1, WANG Shu-Lin1,2,*
1 College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China;
2 State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining 810016, China
Abstract：The yak (Bos grunniens) with yellow body fat showed obvious ability to enrich carotenoids. β- carotene (β-C) is a kind of carotenoids, which plays an important role in carotenoid metabolism of yak. In this study, 15 yaks aged 2~3 years were randomly divided into 5 groups: The control group was fed basic diet; The experimental group included low, medium and high-dose groups, in which the amount of added β-C was 720, 1 440 and 1 620 mg/d, respectively; Positive control was added β-C with 3 440 mg/d. After β-C feeding for 90 d, the expression differences of carotenoid related regulatory gene β-carotene-15,15'-momoxygenase (BCMO1), β-carotene-9', 10'-dioxygenase (BCDO2) and 4 related metabolic regulatory factors - intestine specific homeobox (ISX), peroxisome proliferator-activated receptor γ (PPARγ), scavenger receptor class B type Ⅰ (SR-B1) and retionic acid receptor (RAR) were measured by qRT-PCR, and correlation between BCMO1, BCDO2 and related metabolic regulators was analyzed by Pearson method. The results showed that, in yak duodenum, the expression of BCMO1 in middle dose group was significantly higher than that of other groups; Low, medium and high dosage had no significant effect on BCDO2 expression; Low dosage of β-C was more favorable for ISX expression; There was significant positive correlation between BCDO2 and PPARγ, SR-B1. In the jejunum, the expression of BCMO1 in low and middle dose groups was significantly higher than that of other groups; BCDO2 expression was not affected with β-C supplementation except for the positive group; There was significant positive correlation between BCDO2 and ISX, PPARγ, RAR. In the ileum, the expression change of BCMO1 and BCDO2 were smooth, and the medium dose group was beneficial to the expression of BCMO1 and BCDO2; The expression trends of the 4 metabolic regulatory factors were different, and low dose was beneficial to the expression of ISX, and medium dose was beneficial to the expression of SR-B1; There was significant positive correlation between BCMO1 and PPARγ, SR-B1. In the cecum, the expression of BCMO1 and BCDO2 was not affected by the supplementation. In the colon, the expression of BCMO1 increased with the supplementation; Except for the positive group, the BCDO2 expression was not affected by β-C supplementation; The expression of ISX and RAR showed the maximum in low dose group. The present study provides basic materials for mechanism study of carotenoid metabolism in ruminants, scientific supplementary feeding of yak and the production of high quality yak meat.
任晓莹, 王树林. 日粮中β-胡萝卜素水平对牦牛肠道BCMO1和BCDO2基因及相关代谢调控因子表达的影响及相关性分析[J]. 农业生物技术学报, 2021, 29(6): 1132-1141.
REN Xiao-Ying, WANG Shu-Lin. Effects of Dietary β-carotene Levels on Gene Expression of BCMO1, BCDO2 and Related Metabolic Regulators in Yak (Bos grunniens) Intestine and Correlation Analysis. 农业生物技术学报, 2021, 29(6): 1132-1141.
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