Transcriptomic Analysis of DF-1 Cells Infected with Infectious bursal disease virus
PENG Xi-Ran1, YU Tian-Qi1, ZHANG Yi-Na2, ZHOU Ji-Yong2, HU Bo-Li1,*
1 MOE Joint International Research Laboratory of Animal Health and Food Safety/Institute of Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; 2 MOA Key Laboratory of Animal Virology/Department of Veterinary Medicine, Zhejiang University, Hangzhou 310058, China
Abstract：Infectious bursal disease virus (IBDV) infection cause IBD in chicken (Gallus gallus), which is an acute, highly contagious and immunosuppressive poultry disease. In order to explore the transcriptome changes in host cells infected by IBDV infection, cellular RNAs extracted from IBDV or mock infected DF-1 cells were used to build a library by using TruSeq® RNA LT Sample Prep Kit v2, and then performed high-throughput sequencing on the Illumina HiSeq 3000 instrument. The transcriptome changes of DF-1 cells were analyzed at 12 h after IBDV infecting, and significantly altered genes were screened. The results showed that there were a total of 3 417 differentially expressed genes with 1 887 upregulated and 1 530 downregulated ((Fold Change, FC)>2 and P≤0.05). Systematic bioinformatics analysis with Gene Ontology (GO) and KEGG, revealed that IBDV infection could cause significant changes in more than 20 signaling pathways, such as immunity, apoptosis and metabolism in DF-1 cells. Among them, cellular immunity and apoptosis pathway might play important roles in IBDV infection and immune regulation mechanisms. Nine differentially expressed genes were randomly selected for qRT-PCR verification, and the changing trend was consistent with that of transcriptome data. This study provides reference information for revealing the molecular mechanism of IBDV pathogenesis and immunosuppression.
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