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2025年4月5日 星期六
农业生物技术学报  2019, Vol. 27 Issue (6): 1101-1109    DOI: 10.3969/j.issn.1674-7968.2019.06.016
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
传染性法氏囊病病毒感染DF-1细胞的转录组学分析
彭曦冉1, 俞天奇1, 张宜娜2, 周继勇2, 胡伯里1,*
1 南京农业大学 动物医学院 免疫研究所/教育部动物健康与食品安全国际联合实验室,南京 210095;
2 浙江大学 动物医学系/农业部动物病毒学重点实验室,杭州 310058
Transcriptomic Analysis of DF-1 Cells Infected with Infectious bursal disease virus
PENG Xi-Ran1, YU Tian-Qi1, ZHANG Yi-Na2, ZHOU Ji-Yong2, HU Bo-Li1,*
1 MOE Joint International Research Laboratory of Animal Health and Food Safety/Institute of Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
2 MOA Key Laboratory of Animal Virology/Department of Veterinary Medicine, Zhejiang University, Hangzhou 310058, China
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摘要 传染性法氏囊病病毒(Infectious bursal disease virus, IBDV)感染鸡(Gallus gallus)引起的IBD是一种严重的、高度传染的免疫抑制性疾病。为探索IBDV感染所引起的宿主细胞转录组变化,本研究以IBDV感染鸡成纤维细胞系DF-1,提取细胞总RNA,使用TruSeq® RNA LT Sample Prep Kit v2构建RNA文库,采用Illumina HiSeq 3000平台进行高通量测序,分析IBDV感染12 h引起的DF-1细胞转录组变化,筛选显著变化基因。实验结果显示,差异表达基因总共有3 417条,其中在IBDV感染组中相对高表达的基因有1 887条,相对低表达的基因有1 530条(差异倍数(Fold change, FC)>2且P≤0.05)。对差异基因进行基因本体(Gene Ontology, GO)注释及KEGG信号通路富集分析,发现IBDV感染能够引起DF-1细胞中免疫、凋亡、代谢等20多条信号通路发生显著变化;其中细胞免疫和细胞凋亡通路可能在IBDV感染和免疫调控机制中具有重要作用。随机选择9条差异表达基因进行qRT-PCR验证,变化趋势与转录组数据一致。本研究为揭示IBDV致病及免疫抑制的分子机制提供了参考信息。
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彭曦冉
俞天奇
张宜娜
周继勇
胡伯里
关键词 传染性法氏囊病病毒(IBDV)高通量测序转录组信号通路    
AbstractInfectious bursal disease virus (IBDV) infection cause IBD in chicken (Gallus gallus), which is an acute, highly contagious and immunosuppressive poultry disease. In order to explore the transcriptome changes in host cells infected by IBDV infection, cellular RNAs extracted from IBDV or mock infected DF-1 cells were used to build a library by using TruSeq® RNA LT Sample Prep Kit v2, and then performed high-throughput sequencing on the Illumina HiSeq 3000 instrument. The transcriptome changes of DF-1 cells were analyzed at 12 h after IBDV infecting, and significantly altered genes were screened. The results showed that there were a total of 3 417 differentially expressed genes with 1 887 upregulated and 1 530 downregulated ((Fold Change, FC)>2 and P≤0.05). Systematic bioinformatics analysis with Gene Ontology (GO) and KEGG, revealed that IBDV infection could cause significant changes in more than 20 signaling pathways, such as immunity, apoptosis and metabolism in DF-1 cells. Among them, cellular immunity and apoptosis pathway might play important roles in IBDV infection and immune regulation mechanisms. Nine differentially expressed genes were randomly selected for qRT-PCR verification, and the changing trend was consistent with that of transcriptome data. This study provides reference information for revealing the molecular mechanism of IBDV pathogenesis and immunosuppression.
Key wordsInfectious bursal disease virus (IBDV)    High-throughput sequencing    Transcriptome    Signaling pathway
收稿日期: 2019-02-22     
ZTFLH:  S855  
基金资助:国家自然科学基金(No. 31630077;No. 31502084)
通讯作者: bolihu@njau.edu.cn   
引用本文:   
彭曦冉, 俞天奇, 张宜娜, 周继勇, 胡伯里. 传染性法氏囊病病毒感染DF-1细胞的转录组学分析[J]. 农业生物技术学报, 2019, 27(6): 1101-1109.
PENG Xi-Ran, YU Tian-Qi, ZHANG Yi-Na, ZHOU Ji-Yong, HU Bo-Li. Transcriptomic Analysis of DF-1 Cells Infected with Infectious bursal disease virus. 农业生物技术学报, 2019, 27(6): 1101-1109.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2019.06.016     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2019/V27/I6/1101
 
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