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33
2025年7月29日 星期二
农业生物技术学报  2025, Vol. 33 Issue (6): 1336-1345    DOI: 10.3969/j.issn.1674-7968.2025.06.014
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
威宁鸡PPP3CA基因SNP鉴定及其与生长性状的关联分析
赵中龙1, 冉光友1, 张勇1*, 张习本2, 王智伟1, 艾照碧1, 杨润黔1, 李未博2, 陈泽林2, 叶红英2
1 贵州大学 动物科学学院/高原山地动物遗传育种与繁殖教育部重点实验室, 贵阳 550025;
2 毕节市农业科学院, 毕节 551700
Identification of PPP3CA Gene SNP and Its Association Analysis withGrowth Traits in Weining Chickens (Gallus gallus)
ZHAO Zhong-Long1, RAN Guang-You1, ZHANG Yong1*, ZHANG Xi-Ben2, WANG Zhi-Wei1, AI Zhao-Bi1, YANG Run-Qian1, LI Wei-Bo2, CHEN Ze-Lin2, YE Hong-Ying2
1 College of Animal Science/Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China;
2 Bijie City Academy of Agricultural Sciences, Bijie 551700, China
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摘要 威宁鸡(Gallusgallus)是贵州省地方珍贵家禽种质资源之一。利用候选基因研究威宁鸡生长性状并揭示遗传效应,对威宁鸡遗传改良及分子标记育种具有重要意义。本研究以113只(公58只,母55只)300日龄的威宁鸡为研究对象,采用PCR扩增和直接测序技术鉴定蛋白磷酸酶3催化亚基α(proteinphosphatase3catalyticsubunitα,PPP3CA)基因的单核苷酸多态性(singlenucleotidepolymorphism,SNP)位点,利用Excel统计各位点基因型频率、等位基因频率、有效等位基因数、多态信息含量,并分析各突变位点是否处于Hardy-Weinberg平衡状态;通过在线软件RNAfold进行mRNAs二级结构分析;利用SPSS27.0软件对具有多态性位点的基因型与体尺性状进行关联分析。研究表明,威宁鸡PPP3CA基因外显子6上存在3个SNP位点,即g.151368C>T、g.151389T>C和g.151402T>G,其中g.151368C>T位点发生错义突变导致206位精氨酸变为色氨酸,g.151402T>G位点错义突变导致216位异亮氨酸变为色氨酸。连锁不平衡分析发现,g.151389T>C和g.151368C>T、g.151402T>G位点间存在较强连锁不平衡效应;χ2检验表明,g.151368C>T和g.151389T>C位点符合Hardy-Weinberg平衡,而g.151402T>G位点偏离Hardy-Weinberg平衡。关联性分析发现,g.151402T>G位点TT、GG型个体的体重、体斜长和胸宽显著高于TG个体(P<0.05),TT型个体的胸深显著高于GG、TG型个体(P<0.05),GG型个体的胸深显著高于TG型个体(P<0.05),TT、GG型个体的龙骨长和胫长极显著高于TG型个体(P<0.01);TT、GG型个体胫围显著高于TG型个体(P<0.05)。对3个SNPs位点进行单倍型和双倍型分析,发现共存在4种单倍型和8种双倍型,其中,双倍型H2H3(TCTCTT)和H1H1(TTCCGG)可作为提高龙骨长、胫长、胫围的候选分子标记位点。本研究为威宁鸡生长性状分子标记辅助选择提供了科学依据。
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赵中龙
冉光友
张勇
张习本
王智伟
艾照碧
杨润黔
李未博
陈泽林
叶红英
关键词 威宁鸡(Gallus gallus)蛋白磷酸酶3催化亚基α(PPP3CA)基因单核苷酸多态性(SNP)生长性状关联分析    
Abstract:The Weining chicken (Gallus gallus) is one of the valuable local poultry genetic resources inGuizhou province. It is of great significance for the genetic improvement and molecular marker-assisted breeding of Weining chickens to study their growth traits and uncover their genetic effects using candidate genes. In this study, a total of 113 Weining chickens (58 males and 55 females) aged 300 days were selected asthe study subjects. PCR amplification and direct sequencing were used to identify the SNP locus of PPP3CAgene. Excel was used to calculate genotype frequency, allele frequency, effective allele number and polymorphism information content of each site, and to analyze whether each mutation site was in Hardy- Weinberg equilibrium state. The secondary structure analysis of mRNAs was performed using RNA fold online software. SPSS 27.0 was used to analyze the association between genotypes of different polymorphicloci and body size indexes. The results showed that 3 SNP sites were found on exon 6 of PPP3CA gene inWeining chickens, namely g. 151368C>T, g. 151389T>C and g. 151402T>G, among which missense mutation occurred at g.151368C>T site, resulting in the conversion of arginine at position 206 on mRNA to tryptophan. g. 151402T>G site missense mutation resulting in the conversion of isoleucine at position 216 to tryptophan. Linkage disequilibrium analysis showed that there was a strong linkage disequilibrium effect between g.151389T>C and g. 151368C>T, g. 151402T>G. The χ2 test showed that g. 151368C>T and g. 151389T>C matched Hardy-Weinberg equilibrium, while g. 151402T>G deviated from Hardy-Weinberg equilibrium. At the g.151402T>G site, association analysis showed that TT and GG individuals had significantly higher bodyweight, body slope length, and chest width than TG individuals (P<0.05), TT individuals had significantlyhigher chest depth than GG and TG individuals (P<0.05), GG individuals had significantly higher chest depth than TG individuals (P<0.05), and TT and GG individuals had extremely significantly longer keel and shank length than TG individuals (P<0.01). Individuals of the TT and GG types had considerably larger tibia girth than those of the TG type (P<0.05). Haplotype and diplotype analysis of 3 SNPs showed that there were 4haplotypes and 8 diplotypes. Among them, diplotype H2H3 (TCTCTT) and H1H1 (TTCCGG) could be used as molecular marker sites to improve keel length, shank length and shank girth. This study provides a scientific basis for molecular marker-assisted selection for growth traits of Weining chickens.
Key wordsWeining chicken    Protein phosphatase 3 catalytic subunit α    (PPP3CA) gene    Single nucleotidepolymorphism (SNP)    Growth trait    Association analysis
收稿日期: 2024-06-24     
中图分类号: S433
基金资助:2024年贵州省生态家禽产业技术体系建设任务书项目(GZSTJQCYJSTX-07);毕节市2023年度粤黔东西部协作资金项目;毕节市农业科技计划项目(毕科合[2023]13号);毕节地方优质鸡产业技术创新人才团队建设项目(毕委人领办通[2023]14号)
通讯作者: *gzdxzy2020@163.com   
引用本文:   
赵中龙, 冉光友, 张勇, 张习本, 王智伟, 艾照碧, 杨润黔, 李未博, 陈泽林, 叶红英. 威宁鸡PPP3CA基因SNP鉴定及其与生长性状的关联分析[J]. 农业生物技术学报, 2025, 33(6): 1336-1345.
ZHAO Zhong-Long, RAN Guang-You, ZHANG Yong, ZHANG Xi-Ben, WANG Zhi-Wei, AI Zhao-Bi, YANG Run-Qian, LI Wei-Bo, CHEN Ze-Lin, YE Hong-Ying. Identification of PPP3CA Gene SNP and Its Association Analysis withGrowth Traits in Weining Chickens (Gallus gallus). 农业生物技术学报, 2025, 33(6): 1336-1345.
链接本文:  
https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2025.06.014     或     https://journal05.magtech.org.cn/Jwk_ny/CN/Y2025/V33/I6/1336
 
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