尼罗罗非鱼<em>MYF6</em>基因SNP位点筛选及其与生长性状的关联分析

doi:10.3969/j.issn.1674-7968.2024.02.013

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摘要肌源性因子6 (myogenic factors 6, MYF6)对骨骼肌的起始、发育以及表型维持是不可或缺的,是生长相关性状的候选基因。本研究拟确定MYF6基因的SNPs,并评估这些多态性是否与尼罗罗非鱼(Oreochromis niloticus)的生长性状相关。首先利用PCR和Sanger测序筛选MYF6基因SNPs,通过Sanger测序及SNaPshot方法确认,结果从MYF6基因共筛选到61个SNP位点。进一步从61个位点中挑选多态性大于3%的36个位点,在广东高要亲代群体中利用Sanger测序及SNaPshot方法进行分型分析,并利用SPSS 19软件的一般线性模型(general linear model, GLM)和单因素方差分析(one-way analysis of variance, ANOVA)进行SNP位点与生长性状关联分析。结果显示,36个位点中有2个与高要亲代群体体重相关,4个与全长相关,7个与头长相关,2个与体高相关。将这些生长相关位点在高要子代及番禺群体中进行分型验证,结果显示,在高要子代中,S7插入缺失位点(5620-GAGACGGAGA5629)与体重、体长和体高相关,S24 (C-7388G)位点与体重、体高和体厚相关,S56 (A-11815C)位点与体重、全长、体长、头长、体高和体厚均相关;在番禺群体中获得体重相关SNP位点2个,体长和全长相关位点1个、头长相关位点1个,体厚相关位点2个。将上述与高要子代及番禺群体生长性状相关的位点在海南群体中进一步验证,在雄性群体中获得体重相关位点1个(S29)。双倍型与各群体生长性状关联分析表明,在高要亲代、高要子代以及番禺群体中获得体重相关双倍型各1个。本研究为罗非鱼生长性状改善和选育提供有效的分子标记。

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	高风英
	佟延南
	刘志刚
	曹建萌
	王淼
	衣萌萌
	可小丽
	卢迈新
	朱海

关键词 ：尼罗罗非鱼, 肌源性因子6 (MYF6), SNP, 双倍型, 生长性状

Abstract：The myogenic factor 6 (MYF6) is integral to the initiation and development of skeletal muscle and the phenotype maintenance. Thus, it is candidate gene for growth-related traits. The objectives of this study were to investigate SNPs in MYF6 gene and to evaluate whether these polymorphisms were associated with growth-related traits in Nile tilapia (Oreochromis niloticus). Initially, the SNPs of MYF5 gene were screeded by PCR and Sanger sequencing, and they were confirmed by Sanger sequencing and SNaPshot method. Sixty one SNPs were identified in MYF6 gene, and 36 loci with relative high polymorphism (>3%) were selected from 61 loci for genotyping analysis in Gaoyao parent population using Sanger sequencing and SNaPshot method. The general linear model (GLM) and one way analysis of variance (ANOVA) of SPSS 19 software were used to conduct the association analysis between SNP loci and growth traits. The result showed that 2 of the 36 loci were related to body weight, 4 to full length, 7 to head length, and 2 to body height. These loci, which related to growth-related traits in Gaoyao parent population, were genotyped in Gaoyao offspring and Panyu population. Association analysis between SNP loci and growth-related traits in Gaoyao offspring population showed that S7 (5620-GAGACGGAGA5629) locus was correlated with body weight, body length and body height; S24 (C-7388G) was related to body weight, body height and body thickness; S56 (A-11815C) was correlated with body weight, total length, body length, head length, body height and body thickness. In Panyu population, 2 SNP loci were related to body weight, 1 to body length and full length, 1 to head length, and 2 to body thickness. The above loci, which were related to growth-related traits of Gaoyao offspring and Panyu population, were further verified in Hainan population, and 1 locus associated with body weight was obtained in male population. The correlation analysis between the diploid and the growth-related traits of each population showed that 1 diploid related to body weight was obtained from each of the Gaoyao parent population, the Gaoyao offspring population and the Panyu population, respectively. This study provides effective molecular markers for improving growth traits and breeding of tilapia.

Key words： Nile tilapia Myogenic factors 6 (MYF6) SNP Diploid Growth trait

收稿日期: 2023-04-04

ZTFLH:

S917.4

基金资助:海南省重点研发计划(ZDYF2020096); 财政部和农业农村部：国家现代农业产业技术体系(CARS-46); 2022年省级乡村振兴战略专项资金种业振兴项目(2022-SPY-00-005); 广东省重点领域研发计划(2021B0202020001)

通讯作者: *xiaolike2012@163.com;tyn171575140@163.com

引用本文:

高风英, 佟延南, 刘志刚, 曹建萌, 王淼, 衣萌萌, 可小丽, 卢迈新, 朱海. 尼罗罗非鱼MYF6基因SNP位点筛选及其与生长性状的关联分析[J]. 农业生物技术学报, 2024, 32(2): 396-416.
GAO Feng-Ying, TONG Yan-Nan, LIU Zhi-Gang, CAO Jian-Meng, WANG Miao, YI Meng-Meng, KE Xiao-Li, LU Mai-Xin, ZHU Hai. Screening of SNP of MYF6 Gene in Nile Tilapia (Oreochromis niloticus) and Its Association Analysis with Growth Traits. 农业生物技术学报, 2024, 32(2): 396-416.

链接本文:

https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2024.02.013 或 https://journal05.magtech.org.cn/Jwk_ny/CN/Y2024/V32/I2/396

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