PLAG1 Gene Polymorphism and Its Association Analysis with Growth Performance in Jiangquan Black Pigs (Sus scrofa)
MA Xiao-Yan1,2, WANG Wen-Wen1,2, ZHENG Feng-Ling2, LIU Hui-Xin2, XU Fei2, CHEN Wei1, ZENG Yong-Qing1, TANG Hui1,2,*
1 Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Taian 271018, China; 2 College of Animal Science & Technology, Shandong Agricultural University, Taian 271018, China
Abstract：Plemorphic adenoma gene 1 (PLAG1) plays an important role in regulating the growth and development of livestock and poultry. The purpose of this study was to investigate the relationship between SNP of PLAG1 gene and growth performance in Jiangquan black pig, Jiangquan black pigs weighing about 100 kg were used as research materials. PLAG1 gene mutations in 277 Jiangquan black pigs were detected by Mass ARRAY technology, and the association between SNP and growth traits was analyzed. The results showed that there were 3 SNP in the 5' regulatory region of PLAG1 genes, and 1 in the 3' regulatory region. The polymorphic information content (PIC) of g.75645278A>T and g.75693827A>G sites was 0.35, indicating moderate polymorphism (0.25<PIC<0.50). g.75686279G>A and g.75686320T>C sites showed low polymorphism (PIC<0.25). The genetic analysis showed that only g.75686279G>A locus was in Hardy-Weinberg equilibrium, and the other loci were significantly deviated from Hardy-Weinberg equilibrium (P<0.05). The results of linkage disequilibrium analysis and growth trait correlation showed that the g.75645278A>T and g.75693827A>G sites were completely linked, and had extremely significant effects on days to 100 kg (D100), chest circumference and abdominal circumference (P<0.01). In g.75645278A>T sites, AA individuals with D100 were significantly higher than TT and TA individuals (P<0.05). Chest and abdominal girth of TT and TA individuals were significantly higher than those of AA individuals (P<0.05). Four dominant haplotypes (GCA, GTG, GTA, ATG) and 7 diplotypes were constructed with the loci g.75686279G>A, g.75686320T>C and g.75693827A>G. The 7 combined genotypes had significant effects on D100 (P<0.05), and had extremely significant effects on chest and abdominal circumference (P<0.01). There was extremely significant interaction between haplotype GCA and ATG in chest and abdominal circumference (P<0.01), and significant interaction between haplotype GCA and ATG in body height (P<0.05). There was significant interaction between haplotype GTG and ATG on back height traits (P<0.05). In summary, SNP of PLAG1 gene locus are significantly correlated with growth performance of Jiangquan black pigs, and g.75645278A>T and g.75693827A>G sites could be used as candidate molecular marker sites for growth performance of Jiangquan black pigs. This study provides theoretical support for the breeding of Jiangquan black pigs.
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