Abstract：As an important economic fish in China, the natural germplasm resources of Coilia ectenes are declining rapidly. Exploring the correlation between genetic polymorphisms of C. ectenes fatty acid-binding proteins 7 (CeFABP7) gene with the growth traits plays an important role in the breeding and improvement of growth traits of C. ectenes. Based on the transcriptome data of C. ectenes and gene cloning technology, the cDNA and DNA sequences of CeFABP7 were obtained. The SNPs of CeFABP7 gene was screened by DNA direct sequencing method using 20 individuals that selected from the F3 generation population of C. ectenes with extreme growth characteristics. Then 100 individuals that randomly selected from the same population of C.ectenes were genotyped by DNA direct sequencing or SnaPshot method. The relationship between these SNPs and growth traits of C. ectenes was analyzed by least squares method. The cDNA length of CeFABP7 was 1 295 bp, containing an open reading frame of 399 bp. The genomic DNA of CeFABP7 was 1 899 bp in length containing 3 introns and 4 exons. Six polymorphic sites were detected in 5′-UTR (2), intron 1 (2) and intron 3 (2). The locus g.276C>G was significantly associated with the body length, body height and body weigh traits of C. ectenes (P<0.05), the locus g.306A>C,g.747C>T and g.825C>T were significantly associated with body height and body weigh traits of C. ectenes (P<0.05), while the locus g.1447A>T and g.1532I>D were not significantly associated with 3 growth traits of C. ectenes (P>0.05). The result of population genetic analysis of cultured population of C. ectenes basing on 6 mutation locus showed that the average value of observed heterozygosity (Ho), expected heterozygosity (He) and polymorphism information content (PIC) were 0.257、0.339 and 0.275, respectively. The locus g.276C>G in the CeFABP7 gene was expected to be an important candidate molecular marker for eliminating inferior breeding parents of C. ectenes. This study has theoretical instructive significance for molecular marker assisted breeding in C. ectenes.
于爱清, 施永海, 严银龙. 长江刀鲚FABP7基因遗传多态性及其与生长性状的相关分析[J]. 农业生物技术学报, 2023, 31(9): 1899-1913.
YU Ai-Qing, SHI Yong-Hai, YAN Yin-Long. Genetic Polymorphisms of FABP7 Gene and Their Correlative Analysis with Growth Traits in Coilia ectenes. 农业生物技术学报, 2023, 31(9): 1899-1913.
[1] 艾锦新, 龙安炬, 罗卫星, 等. 2020. 黔北麻羊不同组织中FABP1、FABP3基因表达水平的研究[J].中国畜牧杂志, 56(11): 63-67, 73. (Ai J X, Long A J, Luo W X, et al.2020. Expression levels of FABP1 and FABP3 genes in different tissues of Qianbei Ma goat[J]. Chinese Journal of Animal Science, 56(11): 63-67, 73.) [2] 晁珊珊, 刘茂琪, 马美琳, 等. 2014. 鲤A-FABP基因的克隆及其表达谱研究[J].西北农业学报, 23(6): 43-47. (Chao S S, Liu M Q, Ma M L, et al.2014. Cloning and expression profiles of adipocyte-type fatty acid-binding protein gene in Cyprinus carpio[J]. ACTA Agriculturae Boreali-occidentalis Sinica, 23(6): 43-47.) [3] 陈叶雨, 刘亚, 赖见生, 等. 2020. 达氏鲟心型脂肪酸结合蛋白的基因克隆及表达分析[J]. 农业生物技术学报, 28(1): 139-149. (Chen Y Y, Liu Y, Lai J S, et al.2020. Gene cloning and expression analysis of heart-type fatty acid-binding protein in Acipenser dabryanus[J]. Journal of Agricultural Biotechnology, 28(1): 139-149.) [4] 邓平平, 施永海, 张根玉, 等. 2014. 磁珠富集法分离刀鲚微卫星标记[J]. 生物技术通报, 6: 145-149. (Deng P P, Shi Y H, Zhang G Y, et al.2014. Isolation of microsatellite in C.ectenes by magnetic beads[J]. Biotechnology Bulletin, 6: 145-149.) [5] 邓平平, 袁新程, 施永海. 2021. 1龄刀鲚一般营养成分和脂肪酸组成[J].广东海洋大学学报, 41(4): 128-133. (Deng P P, Yuan X C, Shi Y H.2021. Nutrient composition and fatty acid analyses in muscles of one-year-old Coilia nasus[J]. Journal of Guangdong Ocean University, 41(4): 128-133.) [6] 高妍, 张永宏, 许艳丽, 等. 2010. 松辽黑猪L-FABP基因外显子2多态性及与肉质性状的相关性分析[J]. 中国兽医学报, 30(8): 1129-1132. (Gao Y, Zhang Y H, Xu Y L, et al.2010. Association analysis between polymorphism of Songliao black pig L-FABP gene in exon 2 and meat quality traits[J]. Chinese Journal of Veterinary Science, 30(8): 1129-1132.) [7] 韩英, 陈晓婷, 王琨, 等. 2015. 兴凯湖翘嘴鲌H-FABP基因的克隆及组织表达分析[J]. 大连海洋大学学报, 30(2): 120-124. (Han Y, Chen X T, Wang K, et al.2015. Cloning and expression of H-FABP gene in tissues of Culter alburnus in Xingkai lake[J]. Journal of Dalian Fisheries University, 30(2): 120-124.) [8] 李明明, 吴庆伟, 邓玉婷, 等. 2022. 藏羊BMPR-ⅠA基因多态性及其与产羔性状关联分析[J].畜牧与兽医, 54(6): 18-22. (Li M M, Wu Q W, Deng Y T, et al.2022. Polymorphism of the BMPR-I A gene and its correlation with lambing traits in Tibetan sheep[J]. Animal Husbandry & Veterinary Medicine, 54(6): 18-22.) [9] 廖玉英, 叶超霞, 尹嘉琪, 等. 2014. 暗纹东方鲀心型脂肪酸结合蛋白H-FABP基因克隆与表达分析[J]. 水产学报, 38(11): 1808-1817. (Liao Y Y, Ye C X, Yin J Q, et al.2014. Molecular cloning and feature analysis of heart-type fatty acid-binding protein H-FABP gene from obscure puffer (Takifugu obscurus)[J]. Journal of Fisheries of China, 38(11): 1808-1817.) [10] 林亚秋, 李瑞文, 郑玉才, 等. 2011. 齐口裂腹鱼和鲤鱼H-FABP基因的克隆及其表达谱[J]. 中国生物化学与分子生物学报, 27(6): 554-560. (Lin Y Q, Li R W, Zheng Y C, et al.2011. Cloning and expression profile of heart-type fatty acid-binding protein in Schizothorax prenanti and Cyprinus carpio[J]. Chinese Journal of Biochemistry and Molecular Biology, 27(6): 554-560. [11] 罗宇婷, 代培, 刘思磊, 等. 2021. 2018年长江安庆段洄游汛期刀鲚年龄结构和生长特征[J]. 广东海洋大学学报, 41(3): 36-43. (Luo Y T, Dai P, Liu S L, et al.2021. Age structure and growth characteristics of Coilia nasus in Anqing section of Yangtze River during fishing season in 2018[J]. Journal of Guangdong Ocean University, 41(3): 36-43.) [12] 施永海, 张根玉, 张海明, 等. 2014. 配合饲料和活饵料喂养刀鲚肌肉营养品质分析与比较[J].动物营养学报, 26(2): 427-436. (Shi Y H, Zhang G Y, Zhang H M, et al.2014. Analysis and comparison of nutritional quality of muscle from Coilia nasus fed formulated feed and live feed[J]. Chinese Journal of Animal Nutrition, 26(2): 427-436.) [13] 施永海, 张根玉, 张海明, 等. 2015. 刀鲚的全人工繁殖及胚胎发育[J]. 上海海洋大学学报, 24(1): 36-43. (Shi Y H, Zhang G Y, Zhang H M, et al.2015. Complete artificial propagation and embryonic development of Coilia nasus[J]. Journal of Shanghai Ocean University, 24(1): 36-43.) [14] 隋修磊, 张玉茹. 2019. 鱼类脂肪酸结合蛋白研究进展[J]. 水产科学, 38(4): 563-574. (Sui X L, Zhang Y R.2019. Research progress of fatty acid binding proteins in fish: A review[J]. Fisheries Science, 38(4): 563-574.) [15] 孙雪, 李胜杰, 杜金星, 等. 2021. 草鱼GHRH基因SNPs的筛选及其与生长性状的关联分析[J]. 农业生物技术学报, 29(5): 963-972. (Sun X, Li S J, Du J X, et al.2021. Screening of SNPs in GHRH and association analysis with growth traits in grass carp (Ctenopharyngodon idella)[J]. Journal of Agricultural Biotechnology, 29(5): 963-972.) [16] 田应志, 崔真华, 牟慧蓉, 等. 2017. 贵州白山羊L-FABP基因遗传变异对生长性状的影响[J]. 山地农业生物学报, 36(2): 6-12. (Tian Y Z, Cui Z H, Mou H R, et al.2017. Influence of genetic variation of L-FABP gene on the growth traits of Guizhou white goat[J]. Journal of Mountain Agriculture and Biology, 36(2): 6-12.) [17] 杨晓, 马文超, 杨金, 等. 2021. 红鳍东方鲀B-FABP基因SNPs筛选及其与生长性状关联分析[J]. 广东海洋大学学报, 41(5): 28-34. (Yang X, Ma W C, Yang J, et al.2021. SNPs screening of B-FABP gene and their association with growth traits in Takifugu rubripes[J]. Journal of Guangdong Ocean University, 41(5): 28-34.) [18] 于爱清, 施永海, 徐嘉波, 等. 2019. 长江刀鲚选育群体转录组EST-SSR的分布特征分析[J]. 渔业科学进展, 40(5):101-109. (Yu A Q, Shi Y H, Xu J B, et al.2019. Characteristic analysis of microsatellites in selected Coilia ectenes using a transcriptome dataset[J]. Progress in Fishery Sciences, 40(5): 101-109.) [19] 于爱清, 施永海, 徐嘉波. 2021. 刀鲚MSTN基因遗传多态性与生长性状的关联分析[J]. 水产科技情报, 48(2): 69-76. (Yu A Q, Shi Y H, Xu J B.2021. Single nucleotide polymorphisms of MSTN gene and its association with growth traits in Coilia ectenes[J]. Fisheries Science & Technology Information, 48(2): 69-76.) [20] 俞菊华, 李红霞, 李建林, 等. 2012. 建鲤FABP3基因分离及其多态性与增重的相关分析[J]. 水产学报, 36(12):20-29. (Yu J H, Li H X, Li J L, et al.2012. Sequences analysis of jlFABP3s and the correlation between polymorphisms and body weight gain in Cyprinus carpio var. jian[J]. Journal of Fisheries of China, 36(12): 20-29.) [21] 俞英, 王栋, 孙东晓, 等. 2011. 鸡L-FABP基因全长cDNA克隆表达及与杂种鸡脂肪沉积的关系[J]. 遗传, 33(7): 763-767. (Yu Y, Wang D, Sun D X, et al.2011. Whole cDNA sequence cloning and expression of chicken L-FABP gene and its relationship with lipid deposition of hybrid chickens[J]. Hereditas, 33(7): 763-767.) [22] 张丽, 农伟伦, 卢建雄, 等. 2019. 八眉猪FABPs主要家族基因单核苷酸多态性筛查及生物信息学分析[J].浙江大学学报: 农业与生命科学版, 45(1): 109-118. (Zhang L, Nong W L, Lu J X, et al.2019. Single nucleotide polymorphism screening and bioinformatics analysis of the main family genes of FABPs in Bamei pig[J]. Journal of Zhejiang University (Agriculture & Life Sciences), 45(1): 109-118.) [23] 张世勇, 王明华, 钟立强, 等. 2017. 斑点叉尾鮰MSTN基因4个SNP位点及其与生长性状的相关性[J]. 江苏农业科学, 45(1): 30-33. (Zhang S Y, Wang M H, Zhong L J, et al.2017. Four single nucleotide polymorphisms of MSTN gene and its association with growth traits in Ictalurus punctatus[J]. Jiangsu Agricultural Sciences, 45(1): 30-33.) [24] 仲寒冰, 朱作言, 樊启昶. 2004. 斑马鱼脑脂肪酸结合蛋白(FABP7)在早期胚胎发育过程中的表达图案[J]. 科学通报, 49(3): 296-297. (Zhong H B, Zhu Z Y, Fan Q C.2004. Expression pattern of brain fatty acid binding protein (FABP7) during the early embryonic development of zebrafish (Danio rerio)[J]. Chinese Science Bulletin, 49(3): 296-297.) [25] Barrett J.2005. Haploview : Analysis and visualization of LD and haplotype maps[J]. Bioinformatics, 21(2): 263-265. [26] Boneva N B, Mori Y, Kaplamadzhiev D B, et al.2010. Differential expression of FABP 3, 5, 7 in infantile and adult monkey cerebellum[J]. Neuroscience Research: The Official Journal of the Japan Neuroscience Society, 68(2): 94-102. [27] Botstein D, White R L, Skolnick M, et al.1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. American Journal of Human Genetics, 32(3): 314-331. [28] Camargo G D, Costa R B, Albuquerque L D, et al.2015. Polymorphisms in TOX and NCOA2 genes and their associations with reproductive traits in cattle[J]. Reproduction, Fertility and Development, 27(3): 523-528. [29] Cho J H, Kim I H.2011. Fish meal-nutritive value[J]. Journal of Animal Physiology and Animal Nutrition, 95(6): 685-692. [30] Hohenlohe P A, Bassham S, Currey M, et al.2012. Extensive linkage disequilibrium and parallel adaptive divergence across three pine stickleback genomes[J]. Philosophical Transactions of the Royal Society B-Biological Sciences, 367(1587): 395-408. [31] Kagawa Y, Banlanjo A U, Ariful I, et al.2019. Role of FABP7 in tumor cell signaling[J]. Advances in Biological Regulation, 71: 206-218. [32] Lai Y Y, Lubieniecki K P, Koop B F, et al.2012. Characterization of the Atlantic salmon (Salmo salar) brain-type fatty acid binding protein (fabp7) genes reveals the fates of teleost fabp7 genes following whole genome duplications[J]. Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function, 504(2): 253-261. [33] Liu R Z, Denovanwright E M, Wright J M.2010. Structure, linkage mapping and expression of the heart-type fatty acid-binding protein gene (fabp3) from zebrafish (Danio rerio)[J]. FEBS Journal, 270(15): 3223-3234. [34] Ma R, Wang L, Yuan F, et al.2018. FABP7 promotes cell proliferation and survival in colon cancer through MEK/ERK signaling pathway[J]. Biomedicine & Pharmacotherapy Biomedecine & Pharmacotherapie, 108: 119-129. [35] Peakall R, Smouse P E.2012. GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research-an update[J]. Bioinformatics, 28(19): 2537-2539 [36] Valizadeh M, Aghasizadeh M, Nemati M, et al.2021. The association between a fatty acid binding protein 1 (FABP1) gene polymorphism and serum lipid abnormalities in the MASHAD cohort study[J]. Prostaglandins, Leukotrienes and Essential Fatty Acids, 172: 102324.
