Correlation Analysis Between MRFs Family Gene Polymorphism and Growth Traits in Adult Nile Tilapia (Oreochromis niloticus)
ZHANG Shi-Biao1,2, CHEN Bing-Lin1,2, ZOU Zhi-Ying1,2, XIAO Wei1,2, ZHU Jing-Lin1,2, YU Jie1,2, LI Da-Yu1,2, YANG Hong1,2,*
1 Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 2 Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture; Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China
Abstract：In fish, muscle weight is a key trait reflecting growth performance. The myogenic regulatory factors (MRFs) family of genes plays an important role in muscle generation and development. This study aims to investigate the correlation between the polymorphism of MRFs family genes and the growth traits of adult Nile tilapia (Oreochromis niloticus). PCR amplification and multiple sequence alignment were performed on the coding regions of myogenic factor 5 (myf5), myf6, myogenic determination gene 1 (myod1), myod2, and myogenin (myog). A total of 26 SNPs were screened from the 5 MRFs family genes, including 7 SNPs in myf5, 8 SNPs in myf6, 6 SNPs in myod1, 2 SNPs in myod2, and 3 SNPs in myog. The correlation analysis between the SNPs and growth traits showed that the N7 (C2543T) site of myf5 was significantly related to body weight (P<0.05), and the N9 (A11440G) site of myf6 was significantly related to body weight, total length, body length, and head length (P<0.05). No other SNP significantly associated with growth traits were found in other gene. Additionally, haplotype analysis identified 3 haplotypes in myf5 significantly associated with body thickness (P<0.05) and 4 haplotypes in myf6 significantly associated with growth traits (P<0.05), while no haplotypes significantly associated with growth traits were found in the other genes. These results expand the evaluation of molecular marker applicability and identify 2 candidate markers for molecular-assisted breeding in adult Nile tilapia, providing a theoretical basis for Nile tilapia breeding and quality improvement.
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