Expression and Localization of Key Factors YAP and TEAD-1 in the Downstream of Hippo Signaling Pathway During Placental Development in Yak (Bos grunniens)
QIAN Wen-Jie1, DU Pei-Yan1, LI Yi-Juan1, ZHOU Ying-Cong1, YAO Ying1, LI Liu-Yang1, YU Zhi-Peng1, LIU Min-Yi1, ZHANG Hong-Qiang1, CUI Yan1,2, YU Si-Jiu1,2, FAN Jiang-Feng1,2,*
1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2 Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep & Goat, Lanzhou 730070, China
Abstract：Yes-associated protein (YAP) and TEA domain transcription factor 1 (TEAD-1) are the core factors of the Hippo signaling pathway and play an important role in regulating cell proliferation and organ growth. In order to detect the expression of YAP and TEAD-1 in different developmental stages of yak (Bos grunniens) placenta, and to explore the physiological role of Hippo signaling pathway in regulating placental cell proliferation and organ growth regulation. In this study, placental tissue samples of pregnant yaks were collected and divided into different developmental stages (<2 months old,3 months old,4 months old,>5 months old) based on fetal head-to-hip distance. The expression of YAP and TEAD-1 was detected and analyzed by qRT-PCR, Western blot (WB) and Immunohistochemistry (IHC) technology were used to detect the expression of these genes. The results showed that YAP and TEAD-1 were mainly expressed in fetal chorionic trophoblast giant cells, columnar trophoblast cells and maternal placental crypt epithelial cells. With the progress of pregnancy, the expression of YAP and TEAD-1 mRNA and protein in yak placenta tissue change significantly, and the change trend of YAP and TEAD-1 was basically the same. The co-expression characteristics and the correlation between the expression level and the placental development stage suggested that YAP and TEAD-1 might be involved in the regulation of yak placental development through the Hippo signaling pathway, and play an important role in the physiological function of placental tissue and pregnancy maintenance. The results provides a theoretical basis for elucidating the mechanism of yak placental development and pregnancy regulation.
钱文洁, 杜培岩, 李一娟, 周应聪, 姚颖, 李柳杨, 余志鹏, 柳民意, 张红强, 崔燕, 余四九, 樊江峰. Hippo信号通路下游关键因子YAP和TEAD-1在牦牛胎盘发育中的表达与定位[J]. 农业生物技术学报, 2024, 32(6): 1342-1352.
QIAN Wen-Jie, DU Pei-Yan, LI Yi-Juan, ZHOU Ying-Cong, YAO Ying, LI Liu-Yang, YU Zhi-Peng, LIU Min-Yi, ZHANG Hong-Qiang, CUI Yan, YU Si-Jiu, FAN Jiang-Feng. Expression and Localization of Key Factors YAP and TEAD-1 in the Downstream of Hippo Signaling Pathway During Placental Development in Yak (Bos grunniens). 农业生物技术学报, 2024, 32(6): 1342-1352.
[1] 樊江峰. 2012. 正常妊娠和分娩及药物流产牦牛胎盘组织的细胞凋亡及其调节机制研究[D]. 博士学位论文, 甘肃农业大学, 导师: 余四九. pp. 27-28. (Fan J F.2012. Apoptosis and its regulatory mechanism in yak placental tissue of normal pregnancy and childbirth and medical abortion[D]. Thesis for Ph.D., Gansu Agricultural University, Supervisor: Yu S J. pp. 27-28.) [2] 范清华, 齐洁, 张钧. 2023. Hippo-YAP信号通路在运动性心肌肥大中的调控作用[J]. 中国组织工程研究, 27(17): 2708-2715. (Fan Q H, Qi J, Zhang J.2023. The regulatory role of Hippo-YAP signaling pathway in exercise-induced cardiac hypertrophy[J]. Chinese Tissue Engineering Research, 27(17): 2708-2715.) [3] 樊尚珩, 李均. 2022. Hippo信号通路效应因子Yes相关蛋白与具有PDZ结合基序的转录共激活因子对肾脏疾病的影响[J]. 临床肾脏病杂志, 22(08): 681-685. (Fan S H, Li J.2022. The effect of Hippo signaling pathway effector Yes-related protein and transcription coactivator with PDZ binding motif on kidney disease[J]. Clinical Journal of Nephrology, 22(08): 681-685.) [4] 韩小红, 樊江峰, 于艺腾, 等. 2018. 妊娠期和分娩前后牦牛胎盘组织形态学变化及细胞凋亡的研究[J]. 农业生物技术学报, 26(10): 1714-1722. (Han X H, Fan J F, Yu Y T, et al.2018. Study on the morphological changes and apoptosis of yak placenta before and after pregnancy and delivery[J]. Journal of Agricultural Biotechnology, 26(10): 1714-1722.) [5] 姜璐璐, 朱晓琴, 王慧, 等. 2023. P38 MAPK在子痫前期患者外周血及胎盘组织中的表达及对TNF-α、IFN-γ的影响[J]. 中国优生与遗传杂志, 31(08): 1604-1609. (Jiang L L, Zhu X Q, Wang H, et al.2023. The expression of p38 MAPK in peripheral blood and placental tissues of patients with preeclampsia and its effect on TNF-α and IFN-γ[J]. Chinese Journal of Eugenics and Genetics, 31(08): 1604-1609.) [6] 马悦, 樊江峰, 何翃闳, 等. 2020. 牦牛胎盘组织细胞凋亡相关蛋白的表达[J]. 农业生物技术学报, 28(01): 72-83. (Ma Y, Fan J F, He H H, et al.2020. The expression of apoptosis-related proteins in yak placenta tissue[J]. Journal of Agricultural Biotechnology, 28(01): 72-83.) [7] 王华倩, 余亚君, 张涛, 等. 2022. YAP1在稽留流产绒毛中的表达及对JAR细胞生物学行为的影响[J]. 绍兴文理学院学报(自然科学), 42(03): 69-74. (Wang H Q, Yu Y J, Zhang T, et al.2022. The expression of YAP1 in missed abortion villi and its effect on the biological behavior of JAR cells[J]. Journal of Shaoxing University (Natural Science), 42(03): 69-74.) [8] 闫洪超, 薛加强, 董红燕, 等. 2006. 妊娠期糖尿病患者胎盘超微结构的研究[J]. 徐州医学院学报, (03): 248-250. (Yan H C, Xue J Q, Dong H Y, et al. 2006. Study on placental ultrastructure in patients with gestational diabetes mellitus[J]. Journal of Xuzhou Medical College, (03): 248-250.) [9] 张克慧, 曹梦醒, 周蒙恩, 等. 2022. 雌激素受体活化及Hippo-YAP信号通路激活与肝脏缺血再灌注损伤相关性研究进展及展望[J]. 中西医结合肝病杂志, 32(05): 475-477. (Zhang K H, Cao M X, Zhou M E, et al.2022. The research progress and prospect of the correlation between estrogen receptor activation and Hippo-YAP signaling pathway activation and liver ischemia-reperfusion injury[J]. Journal of Integrated Traditional Chinese and Western Medicine Hepatology, 32(05): 475-477.) [10] 肇涛澜, 张硕, 钱文峰. 2020. 翻译延伸的顺式调控机理与生物学效应[J]. 遗传, 42(07): 613-631. (Zhao T L, Zhang S, Qian W F.2020. Cis regulation mechanism and biological effects of translation extension[J]. Heredity, 42(07): 613-631.) [11] 赵玉红, 邵伟, 吴淑华, 等. 2022. 星形细胞瘤中YAP1和β-catenin的表达及临床联系[J]. 现代医药卫生, 38(17): 2894-2899. (Zhao Y H, Shao W, Wu S H, et al.2022. The expression and clinical relationship of YAP1 and β-catenin in astrocytoma[J]. Modern Medicine and Health, 38(17): 2894-2899.) [12] Bornhorst D, Abdelilah-Seyfried S.2021. Strong as a Hippo's heart: Biomechanical Hippo signaling during zebrafish cardiac development[J]. Frontiers in Cell and Developmental Biology, 9: 731101. [13] Clark K L, George J W, Przygrodzka E, et al.2022. Hippo signaling in the ovary: Emerging roles in development, fertility, and disease[J]. Endocrine Reviews, 43(6): 1074-1096. [14] Currey L, Thor S, Piper M.2021. TEAD family transcription factors in development and disease[J]. Development, 148(12): dev196675. [15] Dong C, Fu S, Karvas R M, et al.2022. A genome-wide CRISPR-Cas9 knockout screen identifies essential and growth-restricting genes in human trophoblast stem cells[J]. Nature Communications, 13(1): 2548. [16] Du P, Lu B, Zhao L, et al.2022. Expression of key factors of the Hippo signaling pathway in yak (Bos grunniens) mammary gland[J]. Animals (Basel), 12(16): 2103. [17] Huang Z, Zhou J, Leung W T, et al.2020. The novel role of Hippo-YAP/TAZ in immunity at the mammalian maternal-fetal interface: Opportunities, challenges[J]. Biomedicine & Pharmacotherapy, 126: 110061. [18] Kessler C A, Bachurski C J, Schroeder J, et al.2008. TEAD1 inhibits prolactin gene expression in cultured human uterine decidual cells[J]. Molecular and Cellular Endocrinology, 295(1-2): 32-38. [19] Kusama K, Bai R, Sakurai T, et al.2016. A transcriptional cofactor YAP regulates IFNT expression via transcription factor TEAD in bovine conceptuses[J]. Domestic Animal Endocrinology, 57: 21-30. [20] Lalonde-Larue A, Boyer A, Dos S E, et al.2022. The Hippo pathway effectors YAP and TAZ regulate LH release by pituitary gonadotrope cells in mice[J]. Endocrinology, 163(1): bqab238. [21] Landin-Malt A, Benhaddou A, Zider A, et al.2016. An evolutionary, structural and functional overview of the mammalian TEAD1 and TEAD2 transcription factors[J]. Gene, 591(1): 292-303. [22] Lin K C, Moroishi T, Meng Z, et al.2017. Regulation of Hippo pathway transcription factor TEAD by p38 MAPK-induced cytoplasmic translocation[J]. Nature Cell Biology, 19(8): 996-1002. [23] Ma S, Meng Z, Chen R, et al.2019. The Hippo pathway: Biology and pathophysiology[J]. Annual Review of Biochemistry, 88: 577-604. [24] Maejima Y, Zablocki D, Nah J, et al.2023. The role of the Hippo pathway in autophagy in the heart[J]. Cardiovascular Research, 118(17): 3320-3330. [25] Meinhardt G, Haider S, Kunihs V, et al.2020. Pivotal role of the transcriptional co-activator YAP in trophoblast stemness of the developing human placenta[J]. Proceedings of the National Academy of Sciences of the USA, 117(24): 13562-13570. [26] Mishra B, Koshi K, Kizaki K, et al.2013. Expression of ADAMTS1 mRNA in bovine endometrium and placenta during gestation[J]. Domestic Animal Endocrinology, 45(1): 43-48. [27] Moon S, Hwang S, Kim B, et al.2022. Hippo signaling in the endometrium[J]. International Journal of Molecular Sciences, 23(7): 3852. [28] Russell J O, Camargo F D.2022. Hippo signalling in the liver: Role in development, regeneration and disease[J]. Nature Reviews Gastroenterology & Hepatology, 19(5): 297-312. [29] Sawada A, Kiyonari H, Ukita K, et al.2008. Redundant roles of Tead1 and Tead2 in notochord development and the regulation of cell proliferation and survival[J]. Molecular and Cellular Biology, 28(10): 3177-3189. [30] Wang M, Dai M, Wang D, et al.2021. The regulatory networks of the Hippo signaling pathway in cancer development[J]. Journal of Cancer, 12(20): 6216.
