Different Analysis of Leptin and Its Receptor in Skin of Different Parts in Young Yak (Bos grunniens)
ZHAO Peng-Fei1, CUI Yan1,2,*, YU Si-Jiu1,2, LIAO Bo1, HE Jun-Feng1, ZHANG Qian1, XU Hong-Wei1
1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2 Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep & Goat, Lanzhou 730070, China;
Abstract：Leptin (leptin, LP) and its receptor leptin receptor (LEPR) play a key role in food intake and energy consumption control. The role of LP and LEPR in the growth of skin and hair follicles has been gradually proved. In order to explore the distribution characteristics of LP and LEPR in different parts of skin of young yak (Bos grunniens), and their possible roles in different parts of skin, 10 healthy young yaks were selected to collect back, abdomen, axilla and scrotum skin. The exact distribution and expression of LP and LEPR in the skin of back, abdomen, axilla and scrotum of young yaks were studied by qPCR, Western blot and immunohistochemical methods. The results of immunohistochemistry showed that the expression of LP and LEPR was basically the same, mainly distributed in the epidermis, outer root sheath of hair follicles, sweat glands, sebaceous glands, capillary endothelial cells and dermal fibroblasts. The average optical density results showed that the expression levels of LP and LEPR in abdomen and back were significantly higher than those in axilla and scrotum (P<0.05). The qPCR results showed that LP expression in the back and abdomen was significantly higher than that in the axilla and scrotum (P<0.05). The expression of LEPR in the back was significantly higher than that in the other 3 parts (P<0.05); There was no significant difference in abdominal, axilla and scrotum (P>0.05). Western blot results showed that LP expression in abdomen and back was significantly higher than that in axilla and scrotum, and the differences among different parts were significant (P<0.05). The expression of LEPR in the back and abdomen was significantly higher than that in the scrotum and axilla, and the difference was significant among different parts (P<0.05). In this study, the expression of LP and LEPR was detected in young yak skin. The expression of LP and LEPR was basically the same in different parts of skin, mainly in epidermis, outer root sheath of hair follicles, sebaceous glands, sweat glands, capillary endothelial cells and dermal inner layer fibroblasts, but the expression were significant differences in different parts of skin. The results suggest that LP and LEPR may play an important role in the growth of skin and hair follicles. This study provides some basic data for further exploring the regulation mechanism of yak skin and hair follicle growth in plateau environment.
赵鹏飞, 崔燕, 余四九, 廖博, 何俊峰, 张虔, 徐宏伟. 瘦素及其受体在幼年牦牛不同部位皮肤中的差异性分析[J]. 农业生物技术学报, 2023, 31(3): 530-538.
ZHAO Peng-Fei, CUI Yan, YU Si-Jiu, LIAO Bo, HE Jun-Feng, ZHANG Qian, XU Hong-Wei. Different Analysis of Leptin and Its Receptor in Skin of Different Parts in Young Yak (Bos grunniens). 农业生物技术学报, 2023, 31(3): 530-538.
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