Expression and Distribution of NGF and Its Receptor in Different Regions of Diencephalon and Brainstem of Yaks (Bos grunniens) and Cattles (B. taurus)
WU Ya-Juan1, DU Xiao-Hua1,*, LIU Xia2, ZHENG Li-Ping2, LIU Shan-Shan1
1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2 College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
Abstract：By connecting to its high affinity receptor tyrosine protein kinase A (TrkA), nerve growth factor (NGF) plays a crucial physiological role in the adaptive regulation of hypoxic ischemia in central nervous system tissues or cells. The purpose of this study was to investigate the expression, distribution and interaction of NGF and TrkA during adaptation to low oxygen environments in the diencephalon and brainstem of yaks (Bos grunniens). Different areas of the diencephalon and brainstem of yaks and cattles (B. taurus) were collected at different altitudes, and the expression levels and distribution features of NGF and TrkA were examined using qPCR, Western blot, and immunohistochemistry (IHC) methods. The results showed that the expression levels of NGF and TrkA genes and protein were significantly higher in the thalamus of yaks and cattles diencephalon and brainstem than in other tissues (P<0.05); Compared with cattles, NGF gene and protein expression levels in the thalamus and medulla oblongata of yaks were significantly higher than in cattles (P<0.05), but lower in the hypothalamus and pons. There was no significant difference between cattle and yaks in the hypothalamus, but there were considerably increased TrkA gene expression levels in the thalamus, pons, and medulla oblongata in yaks (P<0.05).The levels of TrkA protein in the cantamus, hypothalamus, pons, and medulla oblongata were not significantly different from those of cattle. The distribution characteristics of TrkA in the yaks diencephalon and brainstem coincided with NGF, and the intensity of the immunopositive response to TrkA protein was higher than that of NGF protein. The positive products of NGF and TrkA were primarily distributed in the cytoplasm of pleomorphic cells in the thalamus and hypothalamus, motor neurons in the pons, and neurons in the medulla oblongata. According to the aforementioned findings, the thalamus in the diencephalon and brainstem of yaks was the region of the brain that was most vulnerable to hypoxia. The above results showed that the hypothalamus and pons were more tolerant to hypoxia, while the thalamus and medulla were more sensitive, and increased tolerance to hypoxia in the yak thalamus and medulla by activating related neuroprotective mechanisms that up-regulated NGF expression, whereas TrkA did not play a significant under hypoxic conditions. NGF and TrkA were primarily found in the cytoplasm of neurons and glial cells in various regions of the diencephalon and brainstem, implying that they worked together to exert endogenous neuroprotective effects on neurons and glial cells, thereby protecting brain tissue. This study provides theoretical basis and data reference for further exploring the hypoxic adaptation mechanism of yak brain tissue
吴亚娟, 杜晓华, 刘霞, 郑丽平, 刘珊珊. NGF及其受体在牦牛与黄牛间脑及脑干不同区域中的表达与分布[J]. 农业生物技术学报, 2023, 31(9): 1857-1867.
WU Ya-Juan, DU Xiao-Hua, LIU Xia, ZHENG Li-Ping, LIU Shan-Shan. Expression and Distribution of NGF and Its Receptor in Different Regions of Diencephalon and Brainstem of Yaks (Bos grunniens) and Cattles (B. taurus). 农业生物技术学报, 2023, 31(9): 1857-1867.
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