Expression of YAP and TEAD1 in the Formation, Development and Degeneration of Yak (Bos grunniens) Corpus Luteum
ZHOU Ying-Cong1, WANG Jing-Lei1, ZHANG Tong-Xiang1, DU Pei-Yan1, LI Yi-Juan1, YAO Ying1, QIAN Wen-Jie1, CUI Yan1,2, YU Si-Jiu1,2, FAN Jiang-Feng1,2,*
1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2 Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep & Goat, Gansu Agricultural University, Lanzhou 730070, China
Abstract：Yes-associated protein (YAP) and TEA domain transcription factor 1 (TEAD1) are downstream transcriptional co-activators of the Hippo signaling pathway that play important roles in female reproduction. Thus, in order to investigate the relationship between YAP and TEAD1 and corpora lutea (CL) formation, development and degeneration in yaks (Bos grunniens), the female yak corpus luteum (CL) tissues were collected, and divided into 5 groups according to the process of corpus luteum formation (corpus rubrum stage and lacunose luteum stage), development (pregnancy luteal phase), and degeneration (degenerative luteal phase and white corpus stage). The relative expression of YAP and TEAD1 genes and proteins in luteal formation, development and degeneration were examined by qPCR and Western blot, and the localization of YAP and TEAD1 in corpus luteum formation, development and degeneration were analyzed by immunohistochemistry. The results showed that YAP and TEAD1 were abundantly expressed in luteal tissues, and there were significantly difference in different stages of luteal tissues, especially the relative expressions of YAP and TEAD1 were the highest in the lacunose luteum stage and significantly difference from the other stages (P<0.05). However, YAP and TEAD1 expressions were the lowest in the degenerated luteal tissues. Immunohistochemical results showed that YAP and TEAD1 were expressed mainly in yak granulosa luteal cells, vascular endothelial cells and fibroblasts. The results suggested that the Hippo signaling pathway mediated by YAP and TEAD1 was involved in the formation, development and function of luteal tissue in female yaks by stimulating the proliferation of luteal cells, and was associated with angiogenesis during this process. The results of this study provide a reference for further exploration the role played by Hippo pathway in the regulation of ovarian function and maintenance of pregnancy in yaks.
周应聪, 王靖雷, 张同享, 杜培岩, 李一娟, 姚颖, 钱文洁, 崔燕, 余四九, 樊江峰. YAP和TEAD1在牦牛黄体形成、发育和退化中的表达[J]. 农业生物技术学报, 2023, 31(3): 539-548.
ZHOU Ying-Cong, WANG Jing-Lei, ZHANG Tong-Xiang, DU Pei-Yan, LI Yi-Juan, YAO Ying, QIAN Wen-Jie, CUI Yan, YU Si-Jiu, FAN Jiang-Feng. Expression of YAP and TEAD1 in the Formation, Development and Degeneration of Yak (Bos grunniens) Corpus Luteum. 农业生物技术学报, 2023, 31(3): 539-548.
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