Identification and Expression Analysis of NAC Gene Family in Castor (Ricinus communis)
LI Yan-Xiao1, XIANG Dian-Jun1,*, MAN Li-Li2, LIU Peng1
1 College of Agriculture, Inner Mongolia Minzu University, Tongliao 028000, China; 2 College of Life Science and Food Engineering, Inner Mongolia Minzu University, Tongliao 028000, China
Abstract：As a unique transcriptional regulator of terrestrial plants, NAC transcription factors play an active role in plant adaptation to environment and stress resistance. In this study, a total of 74 NAC transcription factors unevenly distributed on 10 chromosomes were identified in the whole genome of castor (Ricinus communis) by bioinformatics methods, and 13 intergenic replications occurred. Phylogenetic analysis showed that RcNACs and Arabidopsis?thaliana NACs were divided into 8 subclusters, and each subcluster contained a NAM domain and a conserved motif 2, and the gene composition structure was similar. A total of 15 stress-related, hormone-responsive and growth-related promoter elements were identified in the promoter region of RcNACs. The tissue expression profile showed that the 54 RcNACs detected had obvious tissue expression specificity in castor, and 39.8% of the members were highly expressed in the male flowers of castor. The stress expression profile showed that 47.3% and 49.1% of the 61 RcNACs were up-regulated in the early stage of salt and drought stress, respectively, except for 6 genes that did not respond to drought stress and salt stress; the low-temperature expression profile showed that 64.1% of the 39 RcNACs were up-regulated in the early stage of stress. The results of qRT-RCR detection under low-temperature stress showed that 10 RcNACs responded to low-temperature for a long time, and most genes had bimodal expression patterns and peaked at 72 h, indicating that RcNACs were the response genes of castor under stress. This study can provide a theoretical reference for the regulation of RcNACs in castor abiotic stress.
李艳肖, 向殿军, 满丽莉, 刘鹏. 蓖麻NAC基因家族鉴定及表达分析[J]. 农业生物技术学报, 2023, 31(12): 2519-2534.
LI Yan-Xiao, XIANG Dian-Jun, MAN Li-Li, LIU Peng. Identification and Expression Analysis of NAC Gene Family in Castor (Ricinus communis). 农业生物技术学报, 2023, 31(12): 2519-2534.
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