联系我们 加入收藏
 
年期检索 高级检索
33
2025年8月3日 星期日
农业生物技术学报  2023, Vol. 31 Issue (12): 2519-2534    DOI: 10.3969/j.issn.1674-7968.2023.12.007
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
蓖麻NAC基因家族鉴定及表达分析
李艳肖1, 向殿军1,*, 满丽莉2, 刘鹏1
1 内蒙古民族大学 农学院,通辽 028000;
2 内蒙古民族大学 生命科学与食品学院,通辽 028000
Identification and Expression Analysis of NAC Gene Family in Castor (Ricinus communis)
LI Yan-Xiao1, XIANG Dian-Jun1,*, MAN Li-Li2, LIU Peng1
1 College of Agriculture, Inner Mongolia Minzu University, Tongliao 028000, China;
2 College of Life Science and Food Engineering, Inner Mongolia Minzu University, Tongliao 028000, China
全文: PDF (9145 KB)   HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 NAC转录因子作为陆生植物特有转录调控因子,在植物适应环境和抗逆境胁迫过程中发挥着积极的作用。本研究利用生物信息学手段在蓖麻(Ricinus communis)的全基因组中共鉴定到了不均匀分布在10条染色体上的74个NAC转录因子,并且发生了13次基因间的复制。系统进化分析显示,RcNACs与拟南芥(Arabidopsis thaliana) NACs共被分为8个亚簇,每一亚簇中的成员均含有NAM结构域和保守的motif 2,并且基因组成结构类似。在RcNACs的启动子区域共鉴定到了15种胁迫类、激素响应类和生长发育相关的顺式作用元件。组织表达谱表明,被检测到的54个RcNACs在蓖麻中拥有明显的组织表达特异性,且有39.8%的成员在蓖麻的雄花中高表达;胁迫表达谱表明,被检测到的61个RcNACs中除了6个基因不响应干旱胁迫和盐胁迫,其余分别有47.3%和49.1%的基因在盐和干旱胁迫的早期上调表达;低温表达谱显示,39个RcNACs中有64.1%的基因在胁迫早期上调表达。低温胁迫下的qRT-RCR检测结果显示,10个RcNACs对低温应答时间响应持久,多数基因有着双峰表达模式且在胁迫72 h出现表达峰值,表明RcNACs是蓖麻的逆境胁迫响应基因。本研究可为RcNACs在蓖麻非生物胁迫的调控作用中提供理论参考。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
李艳肖
向殿军
满丽莉
刘鹏
关键词 蓖麻NAC系统进化表达谱低温胁迫    
Abstract:As a unique transcriptional regulator of terrestrial plants, NAC transcription factors play an active role in plant adaptation to environment and stress resistance. In this study, a total of 74 NAC transcription factors unevenly distributed on 10 chromosomes were identified in the whole genome of castor (Ricinus communis) by bioinformatics methods, and 13 intergenic replications occurred. Phylogenetic analysis showed that RcNACs and Arabidopsis?thaliana NACs were divided into 8 subclusters, and each subcluster contained a NAM domain and a conserved motif 2, and the gene composition structure was similar. A total of 15 stress-related, hormone-responsive and growth-related promoter elements were identified in the promoter region of RcNACs. The tissue expression profile showed that the 54 RcNACs detected had obvious tissue expression specificity in castor, and 39.8% of the members were highly expressed in the male flowers of castor. The stress expression profile showed that 47.3% and 49.1% of the 61 RcNACs were up-regulated in the early stage of salt and drought stress, respectively, except for 6 genes that did not respond to drought stress and salt stress; the low-temperature expression profile showed that 64.1% of the 39 RcNACs were up-regulated in the early stage of stress. The results of qRT-RCR detection under low-temperature stress showed that 10 RcNACs responded to low-temperature for a long time, and most genes had bimodal expression patterns and peaked at 72 h, indicating that RcNACs were the response genes of castor under stress. This study can provide a theoretical reference for the regulation of RcNACs in castor abiotic stress.
Key wordsCastor    NAC    System evolution    Expression profile    Low-temperature stress
收稿日期: 2022-09-16     
ZTFLH:  S565.6  
基金资助:国家自然科学基金(32060492; 31860389); 内蒙古自治区自然科学基金(2022MS03057)
通讯作者: *xiangdianjun00@126.com   
引用本文:   
李艳肖, 向殿军, 满丽莉, 刘鹏. 蓖麻NAC基因家族鉴定及表达分析[J]. 农业生物技术学报, 2023, 31(12): 2519-2534.
LI Yan-Xiao, XIANG Dian-Jun, MAN Li-Li, LIU Peng. Identification and Expression Analysis of NAC Gene Family in Castor (Ricinus communis). 农业生物技术学报, 2023, 31(12): 2519-2534.
链接本文:  
https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2023.12.007     或     https://journal05.magtech.org.cn/Jwk_ny/CN/Y2023/V31/I12/2519
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn