Gene Family Identification of bHLH Transcription Factors and Its Response to Low Temperature Stress in Ground Cover Chrysanthemum (Chrysanthemum morifolium)
REN Man-Rong, QUAN Ying-Jie, YANG Wen-Ting, HE Zi-Han, ZHANG Jia-Ning, GAO Ri*
College of Agriculture, Yanbian University, Yanji 133002, China
Abstract：Ground cover chrysanthemum (Chrysanthemum morifolium) is a perennial herb of the genus Chrysanthemum in the Compositae family, with neat flowers and diverse colors, which has high ornamental value. The low temperature in the north severely limits the cultivation range of ground cover chrysanthemum. The basic helix-loop-helix (bHLH) transcription factors are widely involved in plant growth and development and abiotic stress response. In this study, ground cover chrysanthemum 'Yingjie' was used as material, and 17 CmbHLHs were cloned based on transcriptome data, with ORF between 603 bp and 1 242 bp, and their physical and chemical properties were predicted. The physical and chemical properties of 17 CmbHLHs showed that 8 proteins were acidic and 9 proteins were basic, and these proteins were all located on the nucleus; The proportion of α-helix in the secondary structure of bHLH protein was 25.49%~51.50%, and the proportion of β-turn was 1.42%~5.88%; miRNA prediction analysis showed that 9 miRNAs might regulate the post-transcriptional expression of 8 CmbHLHs. Using MEME to predict the composition of CmbHLH motifs, a total of 6 conserved sequences were identified, except CmbHLH8 and CmbHLH15, all the other proteins contained motif 1 and motif 2. Phylogenetic analysis showed that 17 CmbHLHs were distributed in 12 bHLH subfamilies. Ground cover chrysanthemum seedlings were treated with low temperature, qPCR analysis found that the expression of 7 CmbHLHs increased under low temperature stress. This study provides references for further research on the function of ground cover chrysanthemum bHLH gene.
任镘蓉, 全英杰, 杨雯婷, 何子涵, 张佳凝, 高日. 地被菊bHLH转录因子基因家族鉴定及其低温胁迫响应[J]. 农业生物技术学报, 2022, 30(1): 50-62.
REN Man-Rong, QUAN Ying-Jie, YANG Wen-Ting, HE Zi-Han, ZHANG Jia-Ning, GAO Ri. Gene Family Identification of bHLH Transcription Factors and Its Response to Low Temperature Stress in Ground Cover Chrysanthemum (Chrysanthemum morifolium). 农业生物技术学报, 2022, 30(1): 50-62.
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