Changes in Photoinhibition and Fatty Acid Composition in the Membrane of Chrysanthemum (Chrysanthemum morifolium) Leaves Under Low Temperature Stress
MIAO Run-Tian1,2, LI Hai-Yan1,2, ZHAO Peng-Fei1,2, HUANG Gan1,2, LI Yong-Hua1,2, LU Jiu-Xing1,2,*
1 School of Landscape Architecture and Art, Henan Agricultural University, Zhengzhou 450002, China; 2 Henan Provincial Engineering Research Center of Quality Flowers and Vegetables Seedling, Zhengzhou 450002, China
Abstract：Low temperature in winter and spring has become an important factor affecting the growth and development of chrysanthemum (Chrysanthemum morifolium). To explore the physiological mechanism of chrysanthemum leaves membrane lipid constituents to low temperature stress response, chrysanthemum variety 'Tangyujinqiu' was used to test the fatty acid composition in cell and thylakoid membrane and its correlation with fatty acids desaturase genes and chlorophyll fluorescence parameters, separately. The results showed in cell membrane that index of unsaturated fatty acid (IUFA) significantly increased with the decrease of temperature. The expression levels of Δ9 stearoyl acyl-carrier-protein desaturase genes (CmSAD), and fatty acid desaturase genes (CmFAD2 and CmFAD7) were not completely consistent with contents of fatty acids. In thylakoid membrane, the content of saturated fatty acids (palmitic acid (C16∶0), stearic acid (C18∶0)) decreased significantly, while the content of unsaturated fatty acids (oleic acid (C18∶1), linoleic acid (C18∶2), linolenic acid (C18∶3)) showed upward trend. Fatty acid desaturase genes regulated unsaturation fatty acid more directly in thylakoid membrane than in cell membrane. The IUFA of thylakoid membrane was significantly correlated with chlorophyll fluorescence parameters, indicated that the unsaturated fatty acids repressed photoinhibition by reducing the electron transfer on the PSⅡ acceptor side and increasing heat dissipation. The expression level of fatty acid desaturase genes were increased by low temperature stress in leaves, meanwhile, unsaturated fatty acid level in cell and thylakoid membrane was promoted by low temperature, and maintained the stability of the membrane, this might alleviate the damage caused by low temperature to photosystem to a certain extent. This study provides a theoretical basis for further exploring the relationship between changes in plant membrane lipid components and photoinhibition under low temperature stress.
苗润田, 李海燕, 赵鹏飞, 黄淦, 李永华, 逯久幸. 低温胁迫下菊花叶片光抑制与膜脂构成变化[J]. 农业生物技术学报, 2022, 30(2): 296-304.
MIAO Run-Tian, LI Hai-Yan, ZHAO Peng-Fei, HUANG Gan, LI Yong-Hua, LU Jiu-Xing. Changes in Photoinhibition and Fatty Acid Composition in the Membrane of Chrysanthemum (Chrysanthemum morifolium) Leaves Under Low Temperature Stress. 农业生物技术学报, 2022, 30(2): 296-304.
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