Sequencing and Structural Characteristics Analysis of Mitochondrial Genome in Rhodeus ocellatus from Ou River
LIU Kai1,*, FENG Xiao-Yu1, WU Yan-Qin2, CHU Tian-Jiang1, XIE Nan1
1 Institute of Fishery Science, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China; 2 Lishui Aquatic Product Technology Promotion Department, Lishui 323000, China
Abstract：Rhodeus ocellatus is a kind of ornamental fish with a fair market prospect due to its bright color and beautiful body. The sequencing and analysis of the mitochondrial genome (mtGenome) of R. ocellatus, and the exploration of mtGenome differences of R. ocellatus from different geographical populations and the phylogenetic position of R. ocellatus in Acheilognathinae subfamily at the mtGenome level are of great significance for the phylogenetic study of R. ocellatus. In this study, PCR amplification, sequencing, and software splicing were used to obtain the complete mtGenome of R. ocellatus from Ou River. The full-length sequence (GenBank No. MW007386) was 16 675 bp, and the base composition was A (28.98%), G (17.09%), C (26.51%), and T (27.42%). There were 13 protein-coding genes (PCGs), 22 tRNAs, 2 rRNAs. The (A+T) content of the mtGenome and PCGs in the mtGenome of R. ocellatus from Ou River was 56.41% and 56.27%, respectively. Both mtGenome and PCGs had noticeable AT preference. The 22 tRNAs, except tRNA-Ser(AGY), had a typical clover structure. Based on BLAST alignment, the sequence identity of R. ocellatus from Ou River was 83.84% with that of R. ocellatus from Dong River, 95.07% with that of R. ocellatus from Japan, 93.11% with that of R. ocellatus with GenBank No. DQ026430, 99.03% with that of Acanthorhodeus chankaensis from Tumen River, 83.72% with that of A. chankaensis from Heilong River, and 83.84% with that of A. chankaensis from Yuan River. In addition, the genetic distance between R. ocellatus from Ou River and A. chankaensis from Tumen River was the closest, followed by R. ocellatus from Japan, and R. ocellatus from Ou River was farther away. Phylogenetic tree was constructed based on complete mtGenomes of 25 species of Acheilognathinae subfamily belonging to 3 genera. R. ocellatus from Ou River was closely related to A. chankaensis from Tumen River, but was relatively distant to R. ocellatus from Dong River. The present study provides reference for the genetic diversity protection and breeding of R. ocellatus from Ou River.
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