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2025年8月3日 星期日
农业生物技术学报  2024, Vol. 32 Issue (10): 2203-2217    DOI: 10.3969/j.issn.1674-7968.2024.10.001
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
水稻miR169基因家族成员的进化及热应激响应研究
吴雪瑜1,2, 沈秋平1,2, 谢裕俊1,2, 陈家怡1,2, 於金生1,2,*
1 浙江农林大学 农作物品种改良技术浙江省重点实验室,临安 311300;
2 浙江农林大学 现代农学院,临安 311300
Research on the Evolution and Response to Heat Stress of miR169 Gene Family Members in Rice (Oryza sativa)
WU Xue-Yu1,2, SHEN Qiu-Ping1,2, XIE Yu-Jun1,2, CHEN Jia-Yi1,2, YU Jin-Sheng1,2,*
1 The Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, Zhejiang A&F University, Lin'an 311300, China;
2 College of Advanced Agricultural Sciences, Zhejiang A&F University, Lin'an 311300, China
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摘要 微小RNA (microRNA, miRNA)是长约18~22个碱基的高度保守的非编码RNA,参与植物应对多种非生物胁迫。前期高通量测序发现miR169家族部分成员响应高温胁迫。本研究采用生物信息学工具预测miR169家族的染色体分布、序列结构特征、系统发育关系和倍增情况;利用qRT-PCR检测高温胁迫处理下多个时间点的水稻(Oryza sativa)苗期miR169家族成员及其靶基因的表达。结果显示,miR169家族有2个主要进化分支,成员扩增方式主要为多次串联重复与染色体片段倍增,位于同一分支的miR169h、miR169l、miR169i和miR169m是水稻进化过程中的活跃基因簇,其中3个成员miR169h、miR169l、miR169i均积极响应热应激,预测的靶基因—核因子Y-A1 (nuclear factor Y-A1, NF-YA1)在高温处理16~24 h呈现表达下调的变化趋势。综上所述,水稻miR169基因家族miR169h、miR169i和miR169l响应水稻不温和热应激,可能在水稻耐热性方面发挥重要作用。本研究为深入探讨miRNA参与水稻热应激的分子调控机制提供基础资料。
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吴雪瑜
沈秋平
谢裕俊
陈家怡
於金生
关键词 水稻miR169基因家族热应激表达分析分子进化    
Abstract:MicroRNAs (miRNAs) are 18~22 nt and highly conserved non-coding RNAs involved in plant response to various abiotic stresses. Some members of the miR169 family were found to respond to heat stress based on previous high-throughput small RNA sequencing. This study used bioinformatic tools to analyze and predict the chromosome distribution, sequence structural characteristics, phylogenetic relationships, and duplication of the miR169 family; qRT-PCR was used to detect the expression of miR169 family members and their target genes in rice (Oryza sativa) seedlings at multiple time points under high-temperature stress treatments. The results showed that the miR169 family had 2 major evolutionary branches, and the members expanded mainly through tandem duplication events and chromosome segmental duplication. MiR169h, miR169l, miR169i and miR169m locating in the same branch were active gene clusters in the evolution of rice. Among them, 3 members, miR169h, miR169l and miR169i, all responded actively to heat stress, and the predicted target gene, nuclear factor Y-A1 (NF-YA1), showed down-regulated expression trend under high temperature treatment for 16~24 h. In summary, the rice miR169 gene family miR169h, miR169i and miR169l responded to intense heat stress in rice and might play an important role in rice heat tolerance. This study provides basic data for in-depth exploration of the molecular regulatory mechanism of miRNA involved in heat stress in rice.
Key wordsRice    miR169 gene family    Heat stress    Expression analysis    Molecular evolution
收稿日期: 2023-11-14     
中图分类号: S511
基金资助:国家自然科学基金(32171931); 中国水稻研究所开放课题(160102)
通讯作者: * jinshyu@zafu.edu.cn   
引用本文:   
吴雪瑜, 沈秋平, 谢裕俊, 陈家怡, 於金生. 水稻miR169基因家族成员的进化及热应激响应研究[J]. 农业生物技术学报, 2024, 32(10): 2203-2217.
WU Xue-Yu, SHEN Qiu-Ping, XIE Yu-Jun, CHEN Jia-Yi, YU Jin-Sheng. Research on the Evolution and Response to Heat Stress of miR169 Gene Family Members in Rice (Oryza sativa). 农业生物技术学报, 2024, 32(10): 2203-2217.
链接本文:  
https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2024.10.001     或     https://journal05.magtech.org.cn/Jwk_ny/CN/Y2024/V32/I10/2203
 
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