Identification and Expression Analysis of GH3 Gene Family Members in Potato (Solanum tuberosum)
ZHU Di, WANG Bing-Bing, HE Miao-Miao*
Academy of Agriculture and Forestry Sciences (Qinghai Academy of Agriculture and Forestry Sciences)/Key Laboratory of Qinghai-Tibetan Plateau Biotechnology of Ministry of Education/Key Laboratory of Potato Breeding of Qinghai, Engineering Research Center of Potato in Northwest Region Ministry of Education, Qinghai University, Xining 810016, China
Abstract：The expression of Gretchen Hagen 3 (GH3) genes plays an important role in plant growth and development and response to biotic stresses. In this study, the potato (Solanum tuberosum) cultivar 'Qingshu 9' as the materical for identification and bioinformatics analysis of GH3 family members, and their expression patterns were analysed in potato different tissues and in response to salicylic acid (SA), Methyl jasmonate (MeJA) and gibberellin A3 (GA3) and Phytophthora infestans stresses. Promoter cis-acting element analyses showed that StGH3 family members contained a large number of hormone-related acting elements, speculated that it had potential function in response to biotic and abiotic stresses. Tissue specific analysis showed that StGH3.4 and StGH3.5 genes were highly expressed in roots, StGH3.8 was highly expressed in stems, and StGH3.10 and StGH3.16 were highly expressed in leaves. StGH3 family members were regulated under SA, MeJA, GA3 and P. infestans stress treatments and showed different expression patterns. The StGH3.1, StGH3.5, and StGH3.8 genes responded more strongly to the P. infestans treatment.This study provides a scientific basis for subsequent studies of StGH3 genes function.
朱迪, 王冰冰, 贺苗苗. 马铃薯GH3基因家族成员的鉴定及表达分析[J]. 农业生物技术学报, 2025, 33(3): 498-512.
ZHU Di, WANG Bing-Bing, HE Miao-Miao. Identification and Expression Analysis of GH3 Gene Family Members in Potato (Solanum tuberosum). 农业生物技术学报, 2025, 33(3): 498-512.
[1] 冯亚亭, 李均一, 唐丽, 等. 2022. 木薯GH3基因家族鉴定及其应答病原菌侵染的表达分析[J]. 分子植物育种, 20(6): 1755-1765. (Feng Y T, Li J Y, Tang L, et al.2022. The Cassava GH3 gene family identification and expression analysis in response to pathogen infection[J]. Molecular Plant Breeding, 20(06): 1755-1765.) [2] 郭玲玲, 张芬, 成浩, 等. 2020. 茶树CsAAPs亚家族基因的克隆与表达分析[J]. 茶叶科学, 40(4): 454-464. (Guo L L, Zhang F, Cheng H, et al.2020. Molecular cloning and expression analysis of CsAAPs gene subfamily in Camellia sinensis[J]. Journal of Tea Science, 40(04): 454-464.) [3] 黎颖. 2008. 海岛棉中类GH3基因GbGH3的克隆和表达研究[D]. 硕士学位论文, 上海交通大学, 导师: 唐克轩, pp. 6-8. (Li Y.2008. Cloning and expression analysis of GH3-like gene GbGH3 in Seaisland cotton (Gossypium barbadense)[D]. Thesis for M.S., Shanghai Jiao Tong University, Supervisor: Tang K X, pp. 6-8.) [4] 李彦霞. 2023. 马铃薯晚疫病防治技术[J]. 当代农机, 23(09): 37-38. (Li Y X.2023. Potato late blight control technology[J]. Contemporary Farm Machinery, 23(09): 37-38.) [5] 龙子轩, 刘伟, 王玉萍, 等. 2023. 芸豆CBF基因家族鉴定及低温胁迫下的表达分析[J]. 农业生物技术学报, 31(12): 2506-2518. (Long Z X, Liu W, Wang Y P, et al, 2023. Identification of the CBF gene family in Phaseolus vulgaris and its expression analysis under low temperature stress[J]. Journal of Agricultural Biotechnology, 31(12): 2506-2518.) [6] 马心萍, 朱迪, 贺苗苗. 2023. 紫色马铃薯StCHI基因的克隆与表达分析[J]. 江西农业学报, 35(03): 12-17, 24.(Ma X P, Zhu D, He M M. 2023. Cloning and expression analysis of StCHI gene in purple potato[J]. Acta Agriculturae Jiangxi, 35(03): 12-17, 24.) [7] 钱因红, 张亚真, 白培贤, 等. 2020. 茶树GH3基因家族的鉴定及表达分析[J]. 茶叶通讯, 47(03): 382-392. (Qian Y H, Zhang Y Z, Bai P X, et al.2020. Identification and expression analysis of GH3 family gene in Camellia sinensis[J]. Journal of Tea Communication, 47(3): 382-392.) [8] 园园, 恩和巴雅尔, 齐艳华. 2023. 植物GH3基因家族生物学功能研究进展[J]. 植物学报, 58(5): 770-782. (Yuan Y, Enhebayaer, Qi Y H.2023. Research advances in biological functions of GH3 gene family in plants[J]. Chinese Bulletin of Botany, 58(5): 770-782.) [9] 郑红梅. 2019. 马铃薯晚疫病的发生原因、症状及防治[J]. 现代畜牧科技, 19(11): 44-45. (Zheng H M.2019. Causes, symptoms and control of late blight in potatoes[J]. Modern Animal Husbandry Science & Technology, 19(11): 44-45.) [10] 周思如, 左同鸿, 刘义梅, 等. 2024. 烟草GH3家族全基因组鉴定及表达分析[J/OL]. 分子植物育种,http://kns.cnki.net/kcms/detail/46.1068.S.20230714.0859.002.html. (Zhou S R, Zuo T H, Liu Y M, et al. 2024. Genome-wide identification and expression analysis of GH3 family in tobacco[J/OL]. Molecular Plant Breeding, http://kns.cnki.net/kcms/detail/46.1068.S.20230714.0859.002.html [11] 朱粉团, 陈世怀. 2023. 马铃薯晚疫病的综合防治措施探讨[J]. 农业技术与装备, 23(04): 163-164, 167.(Zhu F T, Chen S H. 2023. Discussion on comprehensive control measures of potato late blight[J]. Agricultural Technology & Equipment, 23(04): 163-164, 167.) [12] Casanova-Sáez R, Voß U.2019. Auxin metabolism controls developmental decisions in land plants[J]. Trends in Plant Science, 24(8): 741-754. [13] Chen C, Wu Y, Li J, et al.2023. TBtools-II: A "one for all, all for one" bioinformatics platform for biological big-data mining[J]. Molecular Plant, 16(11): 1733-1742. [14] Ding X, Cao Y, Huang L, et al.2008. Activation of the indole-3-acetic acid-amido synthetase GH3-8 suppresses expansin expression and promotes salicylate and jasmonate-independent basal immunity in rice[J]. Plant Cell, 20(1): 228-240. [15] Domingo C, Andrés F, Tharreau D, et al.2009. Constitutive expression of OsGH3.1 reduces auxin content and enhances defense response and resistance to a fungal pathogen in rice[J]. Molecular Plant Microbe Interactions, 22(2): 201-210. [16] Feng S, Yue R, Tao S, et al.2015. Genome-wide identification expression analysis of auxin-responsive GH3 family genes in maize (Zea mays L.) under abiotic stresses[J]. Journal of Integrative Plant Biology, 57(9): 783-795. [17] Fu J, Yu H, Li X, et al.2011. Rice GH3 gene family: Regulators of growth and development[J]. Plant Signaling & Behavior, 6(4): 570-574. [18] Hagen G, Guilfoyle T.2002. Auxin-responsive gene expression: genes, promoters and regulatory factors[J]. Plant Molecular Biology, 49(3): 373-385. [19] He M M, Zhou Y, Ye G J, et al.2021. Serial transcriptome analysis reveals genes associated with late blight resistance in potato cultivar Qingshu 9[J]. Agronomy, 11: 1919. [20] Hoffmann M, Hentrich M, Pollmann S.2011. Auxin-oxylipin crosstalk: Relationship of antagonists[J]. Journal of Integrative Plant Biology, 53(6): 429-445. [21] Hui S, Zhang M, Hao M, Yuan M.2019. Rice group I GH3 gene family, positive regulators of bacterial pathogens[J]. Plant Signaling & Behavior, 14(5): e1588659. [22] Jagadeeswaran G, Raina S, Acharya B R, et al.2007. Arabidopsis GH3-like defense gene 1 is required for accumulation of salicylic acid, activation of defense responses and resistance to Pseudomonas syringae[J]. The Plant Journal, 51(2): 234-246. [23] Jain M, Kaur N, Tyagi A K, et al.2006. The auxin-responsive GH3 family in rice (Oryza sativa L.)[J]. Functional & Integrative Genomics, 6(1): 36-46. [24] Kumar R, Agarwal P, Tyagi A K, et al.2012. Genome-wide investigation and expression analysis suggest diverse roles of auxin-responsive GH3 during development and response to different stimuli in tomato (Solanum lycopersicum)[J]. Molecular Genetics and Genomics, 287(3): 221-235. [25] Li X, Salman A, Guo C, et al.2018. Identification and characterization of LRR-RLK family genes in potato reveal their involvement in peptide signaling of cell fate decisions and biotic/abiotic stress responses[J]. Cells, 7(9): 120. [26] Liu K, Kang B C, Jiang H, et al.2005. A GH3-like gene, CcGH3, isolated from Capsicum chinense L. fruit is regulated by auxin and ethylene[J]. Plant Molecular Biology, 58(4): 447-464. [27] Nobuta K, Okrent R A, Stoutemyer M, et al.2007. The GH3 acyl adenylase family member PBS3 regulates salicylic acid-dependent defense responses in Arabidopsis[J]. Plant Physiology, 144(2): 1144-1156. [28] Staswick P E, Serban B, Rowe M, et al.2005. Characterization of an Arabidopsis enzyme family that conjugates amino acids to indole-3-acetic acid[J]. Plant Cell, 17(2):616-27. [29] Staswick P E, Tiryaki I, Rowe M L.2002. Jasmonate response locus JAR1 and several related Arabidopsis genes encode enzymes of the firefly luciferase superfamily that show activity on jasmonic, salicylic, and indole-3-acetic acids in an assay for adenylation[J]. Plant Cell, 14(6): 1405-1415. [30] Wang F, Xia Z, Zou M, et al.2022. The autotetraploid potato genome provides insights into highly heterozygous species[J]. Plant Biotechnology Journal, 20(10): 1996-2005. [31] Wright R M, Hagen G, Guilfoyle T.1987. An auxin-induced polypeptide in dicotyledonous plants[J]. Plant Molecular Biology, 9(2): 625-634. [32] Xie X F, Huang Q Y, Wu W R.2010. Bioinformatic analysis of the GH3 gene family in plant[J]. Genomics and Applied Biology, 29(5): 829-837. [33] Yuan H, Zhao K, Lei H, et al.2013. Genome-wide analysis of the GH3 family in apple (Malus×domestica)[J]. BMC Genomics, 14(1): 297. [34] Zhang C, Zhang L, Wang D, et al.2018. Evolutionary history of the Glycoside Hydrolase 3 (GH3) family based on the sequenced genomes of 48 plants and identification of jasmonic acid-related GH3 proteins in Solanum tuberosum[J]. International Journal of Molecular Sciences, 19(7): 1850. [35] Zhang Z Q, Li Q, Li Z M, et al.2007. Dual regulation role of GH3.5 in salicylic acid and auxin signaling during Arabidopsis-pseudomonas syringae Interaction[J]. Plant Physiology, 145(2): 450-464. [36] Zhao P, Wang D, Wang R, et al.2018. Genome-wide analysis of the potato Hsp20 gene family: Identification, genomic organization and expression profiles in response to heat stress[J]. BMC Genomics, 19(1): 61.
