辣椒CNGC基因家族鉴定及表达分析

doi:10.3969/j.issn.1674-7968.2025.02.004

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摘要环核苷酸门控离子通道(cyclic nucleotide-gated ion channel, CNGC)在植物生长发育及逆境胁迫响应中发挥着重要作用。为深入探究CNGC基因家族在辣椒(Capsicum annuum)中的功能，本研究对辣椒CNGC家族成员进行鉴定，并进行理化性质、亚细胞定位、系统进化发育、结构特征、启动子顺式作用元件及基因表达模式的分析。结果表明，在辣椒全基因组中，共鉴定出14个CaCNGC基因家族成员，在各条染色体上非均匀分布；CaCNGC家族蛋白理论等电点为8.64~9.61，分子量为72.87~91.49 kD，14个成员的不稳定系数均在40以上，总平均亲水性均为负值，表明其均为不稳定的亲水蛋白；所有成员均定位于质膜，具有跨膜结构；CaCNGC上游序列的各种顺式调控元件显示了其在响应激素变化和非生物胁迫等多种刺激中的可能功能；转录组数据分析显示，大多数CaCNGC基因家族成员在辣椒叶、花、果等组织中的表达量都很低甚至不表达，在低温、高温、脱落酸、赤霉素、过氧化氢、吲哚乙酸、茉莉酸和盐胁迫下都有表达；qRT-PCR分析显示，CaCNGC2、CaCNGC7和CaCNGC10基因在低温和高温胁迫下的表达持续上调，可能参与辣椒对温度胁迫的响应。本研究为进一步了解CaCNGC基因家族的功能提供了理论参考，为解析辣椒温度耐受性机制提供新的见解。

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	王田田
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关键词 ：辣椒, 环核苷酸门控离子通道(CNGC), 基因家族, 生物信息学分析, 表达分析

Abstract：Plant cyclic nucleotide-gated ion channel (CNGC) plays an important role in plant growth, development and stress response. In order to investigate the function of CNGC gene family in pepper (Capsicum annuum), the CNGC family members of pepper were identified, and the physical and chemical properties, subcellular localization, phylogenetic development, structural characteristics, promoter cis-acting elements and gene expression patterns were analyzed. The results showed that a total of 14 CaCNGC gene family members were identified in pepper, which were not uniformly distributed on each chromosome. The isoelectric point of CaCNGC was 8.64~9.61, the molecular weight was 72.87~91.49 kD. The instability index was greater than 40, indicating that 14 members of CaCNGC were unstable proteins. All CaCNGC proteins were located in the plasma membrane, had transmembrane structures, and were hydrophilic proteins. The analysis of cis-element in promoter showed that, most of the CaCNGC contained cis-elements related to growth and development, hormone regulation, and abiotic stresses response. Transcriptome data analysis showed that most members of CaCNGC had low or no expression in leaves, flowers and fruits of pepper. Most members of CaCNGC were expressed under low temperature, high temperature, abscisic acid, gibberellin, hydrogen peroxide, indoleacetic acid, jasmonic acid and salt stress. qRT-PCR analysis showed that the expression of CaCNGC2, CaCNGC7 and CaCNGC10, was up-regulated under low and high temperature stress, which might be involved in the response of pepper to temperature stress. These results provide a theoretical reference for further investigating the function of CaCNGC gene family, and new insights into the molecular mechanisms of pepper tolerance to temperature stress.

Key words： Pepper Cyclic nucleotide-gated ion channel (CNGC) Gene family Bioinformatic analysis Expression analysis

收稿日期: 2024-02-29

中图分类号： S641.3

基金资助:山西省基础研究计划青年科学研究项目(202203021212451)；山西省重点研发计划(202202140601006)；山西省科技创新人才团队(202304051001018)；山西省博士毕业生、博士后研究人员来晋工作奖励经费科研项目(SXBYKY2022015)；山西农业大学博士科研启动项目(2021BQ115)

通讯作者: * wangjing315@sxau.edu.cn

引用本文:

王田田, 常雪瑞, 王静. 辣椒CNGC基因家族鉴定及表达分析[J]. 农业生物技术学报, 2025, 33(2): 271-284.
WANG Tian-Tian, CHANG Xue-Rui, WANG Jing. Identification and Expression Analysis of CNGC Gene Family in Pepper (Capsicum annuum). 农业生物技术学报, 2025, 33(2): 271-284.

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https://journal05.magtech.org.cn/Jwk_ny/CN/10.3969/j.issn.1674-7968.2025.02.004 或 https://journal05.magtech.org.cn/Jwk_ny/CN/Y2025/V33/I2/271

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