Identification and Expression Analyses of PRX Genes Family in Maize (Zea mays)
HAO Li-Dong1,2,3, XU Lei4,*, YAN Zhi-Bin5, LI Shi-Feng5, SHI Yun-Qiang6
1 Postdoctoral Research Station of Gansu Dunhuang Seed Industry Group Co., Ltd, Jiuquan 735000, China; 2 Postdoctoral Mobile Station of Biology, Lanzhou University, Lanzhou 730030, China; 3 Qionghai Tropical Crops Service Center, Qionghai 571400, China; 4 Heilongjiang Provincial Academy of Agricultural Sciences, Daqing Branch, Daqing 163319, China; 5 Research Institute of Gansu Dunhuang Seed Industry Group Co. Jiuquan 735000, China; 6 Heilongjiang Provincial Academy of Agricultural Sciences, Suihua Branch, Suihua 152000, China
Abstract：Class Ⅲ peroxidases (PRXs) are a family of genes unique to higher plants that are widely involved in catalyzing hydrogen peroxide, phenolic compounds and reactive oxygen species. To date, the study of the PRX gene family at the genome-wide level in Zea mays has not been reported. This study utilized bioinformatic methods to conduct a comprehensive analysis of the PRX gene family at the whole-genome level in maize, and examining their expression patterns in different tissues and under various stress conditions. The results indicated that a total of 90 ZmPRX genes were identified, unevenly distributed across the 10 chromosomes of maize. Specifically, 12 of these genes were found to be involved in 6 segmental duplication events, while 15 genes participated in 6 tandem duplication events. Phylogenetic analysis classified them into 8 branches, with genes in the same branch having similar gene structures and conserved motifs. Cis-element analysis revealed that there were 5 categories of elements distributed in the promoter region of ZmPRX genes, including hormonal responsiveness, stress responsiveness, growth and development, light responsiveness, and universal elements. The expression pattern analysis revealed that the expression of ZmPRX genes exhibited tissue-specific characteristics and were significantly induced under different abiotic stress conditions. Furthermore, under drought stress conditions, the expression level of the ZmPRX gene in the drought-tolerant inbred line 54358 significantly increases, suggesting that this variety possesses strong capabilities in resisting drought stress. This study provides a theoretical basis for further investigating the functional characteristics of PRX genes in maize.
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