A new strain CAU5501 (China patent application No. 200510066114.7), with high mycelial growth rate and high Ganoderma lucidum  polysaccharide-peptide complex (GPSPc) yield was obtained by UV(ultraviolet) induced mutagenesis on protoplasts of its parent strain (CAU55, Ganoderma lucidum).  After genetic stability test, it  was cultivated continuously for 10 generations on PDA slants and shaking bottles, the average growth rate (cm/7 d) of mycelium and the average yield of ex-PSPc (mg/mL) were improved 40.3% and 42.9% respectively compared with CAU55, showing that CAU5501 was a excellent strain with steady property, high mycelial growth rate and high GPSPc yield.  A feeding trial in broiler chicken was carried out to investigate the different effect of GPSPc and antibiotics on immune function, productive performance and carcassmerit of broilers. The data showed that GPSPc played important role on enhancing T-lymphocyte activity (+28.8%) and BSA antibody level (P <0.05), and stimulating the growth of thymus, spleen and bursa of Fabricius (+18.5%) compared with ones fedenramycin. It was found that GPSPc had better effects than enramycin on promoting the daily gain (P<0.01) in 1 to 4 weeks and growth of breast (+6.3%) and thigh muscle (+3.6%), and reducing abdomen fat (-18.6%) of broilers at 7 weeks. Based the comparison of on the efficiency between the two feed additives, it was proved that GPSPc was feasible for substituting antibiotics.

Genetic variation of six microsatellite loci BM4505, BM1905, BM1443, BM415, BM143 and BM711 which were closely correlated with milk composition traits was analyzed in 200 Beijing Holstein cows by nondenaturing polyacrylamide gel electrophoresis. Allele frequency, heterozygosity, polymorphic information content, effective number of alleles of the six microsatellite loci were calculated. Relationships between six microsatellite loci and milk composition traits in Beijing Holstein cows were preliminarily analyzed by least squares linear model. The results indicated that microsatellite BM4505 significantly correlated with fat percentage and dry matter percentage; microsatellite BM1905 significantly correlated with protein percentage and lactose percentage; microsatellite BM1443 significantly correlated with protein percentage; microsatellite BM415 significantly correlated with fat percentage, protein percentage, lactose percentage and dry matter percentage; microsatellite BM143 significantly correlated with protein percentage and lactose percentage; microsatellite BM711 significantly correlated with fat percentage. 225 bp/225 bp at BM4505, 168 bp/168 bp at BM415 and 190 bp/190 bp at BM711 were the most favourable genotypes for fat percentage. 201 bp/201 bp at BM1905, 163 bp/163 bp at BM1443, 168 bp/168 bp at BM415 and 110 bp/110 bp at BM143 were the most favourable genotypes for protein percentage. 201 bp/201 bp at BM1905, 168 bp/168 bp at BM415 and 108 bp/108 bp at BM143 were the most favourable genotypes for lactose percentage. 225 bp/225 bp at BM4505 and 168 bp/168 bp at BM415 were the most favourable genotypes for dry matter percentage.

The polymorphism of the insulin-like growth factor binding protein 3 (IGFBP3) gene in Nanyang cattle, Luxi cattle and Chinese Simmental was analyzed by PCR-SSCP. The results showed that there were no polymorphism detected by the exon 3 of the bovine IGFBP3, and there were three genotypes (AA, AB and BB) detected by the exon 2 of the bovine IGFBP3 in three breeds. The frequency of A allele was  higher than that of B allele in three breeds. The frequency of AA genotype was the highest in three breeds, which Chinese Simmental was 0.6615, the Luxi cattle and Nangyang cattle were 0.4043 and 0.3095 respectively. The polymorphism fragments amplified by primer 1 (the exon 2 of the bovine IGFBP3) were cloned and sequenced. The sequencing results indicated that there was one single nucleotide mutation: T→C at 8 069 bp of IGFBP3 gene, and this mutation resulted in amino acid change: Phe→Leu.