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Evaluating Gene Expression of Peptide Transporter cPepT1
in Small Intestine of Chick by Semi-quantity RT-PCR |
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Abstract A method of semi-quantity RT-PCR was established to evaluate gene expression of peptide transporter in small intestine of chick. The primers of β-actin and cPepT1 were designed according to their gene sequence reported. The cycles and Mg2+ concentrations were optimized by experiment. The results showed that gene expression of cPepT1 varied in intestinal segments, decreased from the proximal to the distal, i.e. the level of the expression of cPepT1 in ileum was higher than that in duodenum and jejunum significantly, no difference was found between duodenum and jejunum.
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Received: 14 July 2005
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