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PCR-SSCP Detection and DNA Sequence Analysis of Exon 10
of Goat Follicle-stimulating Hormone Receptor (FSHR) Gene |
| LANXian-yong；CHENHong；PANChuan-ying；LEIChu-zhao；ZHANGYong-de；YUJiao |
| College of Animal Science and Technology, Northwest Agriculture and Eorestry University, Shaanxi Key Laboratory of |
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Abstract PCR-SSCP was applied to detect single nucleotide polymorphism (SNP) of exon 10 of the follicle-stimulating hormone receptor (FSHR) gene for 173 goats (Capra hircus), including Xinong Sanen Dairy goat, Guangzhong goat, Shaannan White goat, Angera and Boer goat breeds. The results demonstrated monopolymorphism. After sequenced, goat FSHR gene exon10 DNA sequence was firstly acquired ( GenBank Accession No.DQ069909 and DQ069910). DNA sequence analysis showed that molecular mechanism of monopolymorphism was probably due to no nucleotide mutation from C to T or other mutations in 120th nucleotide position. Comparing nucleotide sequences of FSHR gene exon 10 for goat, sheep (Ovisaries L) and bovine (Bos Taurus), the maximal similarity and the minimal divergence was 99.3% and 3.4%, respectively. The molecular phylogenetic tree of goat, sheep and bovine constructed by FSHR gene exon 10 sequences showed that individuals within species were clustered together, respectively; Sheep and goat had the nearest relative relationship, and then clustered with bovine. The result implies that FSHR gene exon 10 sequence is suitable for constructing molecular phylogenetic tree among species.
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Received: 31 May 2005
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Corresponding Authors:
CHEN Hong
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ZHAO Zhi-Dong, TIAN Hong-Shan, JIANG Yan-Yan, SHI Bin-Gang, LIU Xiu, LI Xu-Peng, WANG Deng-Zhe, CHEN Jin-Lin, HU Jiang. Polymorphisms of ACSL1 Gene Promoter and Their Association Analysis with Milk Quality Traits in Yak (Bos grunniens)[J]. 农业生物技术学报, 2019, 27(9): 1596-1603. |
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