Construction of Forward and Reverse Subtracted cDNA Libraries between Longissimus Muscle Tissue of Meishan and Large White×Meishan Crossbred Pigs and ESTs Analysis
Abstract In order to discover the molecular mechanism of heterosis from the poit of gene differential expression, forward and reverse subtracted cDNA libraries were constructed between longissimus muscle tissue of Meishan and Large White×Meishan crossbred pigs both with an efficiency of 210 times. Six hundred and ten avilable clones were selected in the library between Large White×Meishan crossbred (subtracted) and Meishan pigs. Forty-eight positive clones were selected by Dot blotting method. The sequencing results revealed that 28 ESTs of 37 genes represented known genes respectively. The nine ESTs had no homology from NCBI. Five hundred and eighty avilable clones were selected in the library between Meishan (subtracted) and Large White×Meishan crossbred pigs. Forty-five positive clones were selected by Dot blotting method. The sequencing results revealed that 26 ESTs of 34 genes represented reported genes respectively. The eight ESTs had no homology from NCBI.
Construction of Forward and Reverse Subtracted cDNA Libraries between Longissimus Muscle Tissue of Meishan and Large White×Meishan Crossbred Pigs and ESTs Analysis
