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Isolation and Analysis of Genes Induced by Rehydration after Serious
Drought in Broomcorn Millet (Panicum miliaceum) by Using SSH |
| LIN Fan-yun；HU Yin-gang；SONG Guo-qi；ZHANG Hong；LIU Tian-ming；HE Bei-ru |
| College of Agronomy, Yangling Branch of China National Wheat Improvement Center, Shaanxi Key Laboratory of Molecular Biology for Agriculture，Northwest Agriculture and Forestry University, Yangling 712100, China |
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Abstract In order to investigate the molecular mechanism of broomcorn millet (Panicum miliaceum) on the condition of rehydration after serious drought, a forward subtracted cDNA library was constructed between normal watering leaves of broomcorn millet and rehydration ones after serious drought conditions by using SSH(suppression subtraction hybridization) technique. A total of 60 positive clones were randomly picked out from the subtracted library and sequenced, and redundancy sequences were removed after sequence alignment. Based on the result of sequence homologous comparison and function querying, 32 EST sequences were highly homologous with known EST. Most of those sequences were related to abiotic or biotic stresses in plants. Of those sequences, 11 EST sequences were homologous with mice liver ESTs of partial hepatectomy. The blast result of proteins revealed, there were 28 EST sequences were similar to known proteins. The functions of those proteins mainly involve signal transduction, transcription and protein processing. This experiment displays that a series of specific genes are induced and expressed during the rehydration stage after serious drought.
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Received: 19 September 2005
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