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Abstract According to the conservative sequences of known low-molecular-weight glutenin subunit (LMW-GS) genes, the PCR primers were designed, and the full coding regions of LMW-GS genes were amplified from the genomic DNA of Aegilops triuncialis L. Two novel genes with the typical structure of LMW-GS genes, GenBank accession AY841016 and AY841017, were identified from the expected 900 and 1 065 bp amplification products, respectively. The coding region of AY841017 was 1 065 bp, and encoded a mature protein with 322 amino acid residues. The first cysteine residue of AY841017 was observed at position 13 in the repetitive domains. In the repetitive domain, the two hydrophobic motifs of AY841017 were PIIIL and PVIIL. A repeat motif with 13 glutamines (Q) was found in the repetitive domain of AY841017. Due to two stop codons in its coding region, AY841016 was a pseudogene. It was found that AY841017 was highly similar to those encoded by Glu-D3 locus.
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Received: 28 September 2005
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