农业生物技术学报

联系我们

加入收藏

年期检索

高级检索

友情链接

更多....

农业生物技术学报 2003年 11卷 6期刊出日期：2003-11-20

研究论文

花药培养获得转Bt基因抗虫水稻纯系*

To Obtain Insect-resistant Transgenic Rice Lines
with Homozygous Bt Gene Using Anther Culture

2003, 11(6): 561-565 | doi: | Full text (HTML) (1 KB) |

PDF (287 KB) ( 170 )

摘要

应用RAMP标记研究黑麦属遗传多样性*

Genetic Diversity of Secale Based on RAMP Markers

尚海英郑有良** 魏育明吴卫颜泽洪

2003, 11(6): 566-571 | doi: | Full text (HTML) (1 KB) |

PDF (393 KB) ( 285 )

摘要

cDNA微阵列检测水稻幼苗空间特异表达基因*

Identifying the Spatial Specifically Expressed Genes in Rice（Oryza sativa ）Seedlings by Using cDNA Microarray

孙亮先董海涛庄晓峰张峰李德葆**

2003, 11(6): 582-587 | doi: | Full text (HTML) (1 KB) |

PDF (353 KB) ( 198 )

摘要

基因芯片检测转基因油菜

Detection of Transgenic Rapeseeds with DNA Microarray

黄文胜1 潘良文3 粟智平1 缪海珍2 朱水芳1* 李瑶2

2003, 11(6): 588-592 | doi: | Full text (HTML) (1 KB) |

PDF (357 KB) ( 291 )

摘要

甜橙β-胡萝卜素羟化酶cDNA克隆及其瞬间反义表达*

Cloning of β-carotene Hydroxylase cDNA from
Orange (Citrus sinensis ) and Its Transient Antisense Expression

徐昌杰陈昆松张上隆秦巧平

2003, 11(6): 593-597 | doi: | Full text (HTML) (1 KB) |

PDF (353 KB) ( 222 )

摘要

不同基因型刺梨及其近缘种亲缘关系的RAPD分析*

Characterization of Genotypes and Genetic Relationship of Cili
(Rosa roxburghii ) and Its Relatives Using RAPD Markers*

文晓鹏1，2 邓秀新1**

2003, 11(6): 605-611 | doi: | Full text (HTML) (1 KB) |

PDF (482 KB) ( 230 )

摘要

鸡胚致死孤儿病毒Ⅲa蛋白基因的克隆、表达及其抗原性鉴定

Cloning and Expression of Protein Ⅲａ Gene of Chicken Embryo Lethal
Orphan Virus(CELOV) and Identification of Its Antigenicity

周斌1 齐香荣1 杨耀武2 芦银华1 陈溥言1*

2003, 11(6): 616-619 | doi: | Full text (HTML) (1 KB) |

PDF (262 KB) ( 225 )

摘要

StAR蛋白在仔猪睾丸组织中的表达*

Expression of StAR Protein in the Early Piglet Testes

王鲜忠孙燕吴建云李军锋张家骅**

2003, 11(6): 624-627 | doi: | Full text (HTML) (1 KB) |

PDF (394 KB) ( 259 )

摘要

四川小麦品种贮藏蛋白位点及SSR分析*

Analyses of Storage Protein Sites and SSR in Sichuan Registered Wheat Cultivars

王春梅傅体华** 任正隆

2003, 11(6): 598-604 | doi: | Full text (HTML) (1 KB) |

PDF (221 KB) ( 277 )

摘要

牛α1，3-半乳糖转移酶基因部分片段的克隆
及无启动子打靶载体的构建*

Cloning of the Genomic Fragments of Bovineα1,3-galactosyltransferase
Gene and Construction of a Promotorless Targeting Vector

陈立栋李雪辉胡宏宇安晓荣林爱星**

2003, 11(6): 612-615 | doi: | Full text (HTML) (1 KB) |

PDF (327 KB) ( 222 )

摘要

不同因素对牛-兔种间重构卵融合率的影响*

Effect of Some Factors on the Fusion Rate of Bovine-rabbit
Interspecies Reconstructed Eggs

章孝荣1，2 刘亚2 张运海3 陈大元1 廉莉1 李劲松1 金仁桃2 章志国2 章美玲2 汪存利2

2003, 11(6): 620-623 | doi: | Full text (HTML) (1 KB) |

PDF (251 KB) ( 258 )

摘要

海南根瘤菌nodABC基因DNA片段的克隆、测序和功能验证*

Cloning, Sequencing and Functional Testing of the DNA Fragment
Containing nodABC Genes of Rhizobium hainanense

温尚昆李小红王磊杨苏声**

2003, 11(6): 628-632 | doi: | Full text (HTML) (1 KB) |

PDF (531 KB) ( 184 )

摘要

苜蓿根瘤菌聚羟丁酸代谢突变体的竞争生长和结瘤能力
以及外源生物素的影响

Growth and Nodulation Competitiveness of Poly-3-hydroxybutyrate Metabolism Mutants of Sinorhizobium meliloti and the Effects of Exogenous Biotin

戴美学1 武波2* 柏学亮2 张成刚3 马庆生2

2003, 11(6): 633-637 | doi: | Full text (HTML) (1 KB) |

PDF (328 KB) ( 217 )

摘要

植物表达载体构建和转基因烟草抗除草剂及耐盐性的分析*

Construction of Plant Expression Vector and Analysis of
Herbicide-resistance and Salt -tolerance of Transgenic Tobacco

王先艳单晓昳杨爱芳张举仁**

2003, 11(6): 554-560 | doi: | Full text (HTML) (1 KB) |

PDF (454 KB) ( 296 )

摘要

菜苔小孢子培养及再生植株的倍性鉴定*

Microspore Culture and Ploidy Identification of Regenerated Plant in Chinese
Flowering Cabbage (Brassica rapa ssp. parachinensis )

顾宏辉1,2 朱金庆1 张国庆2 周伟军2**

2003, 11(6): 572-576 | doi: | Full text (HTML) (1 KB) |

PDF (328 KB) ( 212 )

摘要

利用小麦微卫星引物建立偃麦草Ee染色体组特异SSR标记

Development of Specific SSR Marker for Ee-genome of
Thinopyrum sp. by Using Wheat Microsatellites

尤明山1 李保云1 田志会2 唐朝晖1 刘守斌1 刘广田1

2003, 11(6): 577-581 | doi: | Full text (HTML) (1 KB) |

PDF (297 KB) ( 299 )

摘要

综述

植物雄配子体转化技术及应用*

A New Approach to Obtain Transgenic Plants—Male Germ Line Transformation

王宏芝

2003, 11(6): 638-641 | doi: | Full text (HTML) (1 KB) |

PDF (219 KB) ( 169 )

摘要

微生物几丁质酶的分子生物学研究及其应用*

Microorganism Chitinases: Molecular Biology and Potential Use as Biopesticides

沙莉苏争先黄小红邱君志关雄**

2003, 11(6): 642-647 | doi: | Full text (HTML) (1 KB) |

PDF (619 KB) ( 258 )

摘要

研究简报

甜瓜果实发育早期cDNA文库的构建

Construction of a cDNA Library of Melon Fruit at Early Stage

于喜艳1 赵双宜2 何启伟3 孔庆国1*

2003, 11(6): 652-653 | doi: | Full text (HTML) (1 KB) |

PDF (154 KB) ( 229 )

摘要

甘蓝Ogura胞质雄性不育基因的RAPD标记筛选*

Screening a RAPD Marker Related to Ogura Cytoplasm Male Sterile Gene in Cabbage*

曹必好雷建军陈国菊

2003, 11(6): 648-649 | doi: | Full text (HTML) (1 KB) |

PDF (256 KB) ( 203 )

摘要

PCR法快速检测小麦回交后代HMW-GSDx5基因*

Rapid Determination of HMW-GS Dx5 Gene for Early Evaluation of
Backcross Progeny in Wheat by PCR

张晓科1** 魏益民2，3 郭月霞1 刘斌1 高荣村1

2003, 11(6): 650-651 | doi: | Full text (HTML) (1 KB) |

PDF (159 KB) ( 190 )

摘要

利用PCR在已克隆基因末端添加终止密码的方法与应用*

Methods and Applications of Inserting a Stop Codon into a Cloned Gene with PCR

常维山1 Henry De Greve2 Nele Buys2 Jean Pierre Hernal Steens2**

2003, 11(6): 654-655 | doi: | Full text (HTML) (1 KB) |

PDF (134 KB) ( 209 )

摘要

副溶血弧菌（Vibrio parahaemolyticus）极鞭毛蛋白FlaB基因的克隆与序列分析*

Cloning and Sequencing of FlaB Gene of Polar Flagellin of Vibrio parahaemolyticus

覃映雪苏永全** 周化民王军王德祥

2003, 11(6): 656-657 | doi: | Full text (HTML) (1 KB) |

PDF (217 KB) ( 245 )

摘要

专家论坛

中国马氏珠母贝遗传育种的现状与展望

Present Status and Future Prospects for Genetic Breeding of
Pearl Oyster (Pinctada martensii ) in China

王爱民** 石耀华

2003, 11(6): 547-553 | doi: | Full text (HTML) (1 KB) |

PDF (463 KB) ( 487 )

摘要