农业生物技术学报

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农业生物技术学报 2004年 12卷 1期刊出日期：2004-01-20

研究简报

木质素合成关键酶基因CCR的克隆及其反义表达载体的构建

Cloning of Cinnamoyl- CoA Reductase (CCR ) Gene and the
Construction of Antisense-CCR Expression Vecto

黄锐之王伏林郭晨悦刘智宏陈锦清**

2004, 12(1): 116-117 | doi: | Full text (HTML) (1 KB) |

PDF (195 KB) ( 165 )

摘要

专家论坛

不同国家和地区转基因产品标识管理政策的比较

Comparison of Labeling Policy of Genetically Modified
Products in Different Countries and Territories

金芜军1 贾士荣1 彭于发2

2004, 12(1): 1-7 | doi: | Full text (HTML) (1 KB) |

PDF (241 KB) ( 673 )

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研究论文

快速获得葫芦科核糖体失活蛋白新基因

Rapid Screening for Novel Genes of Ribosome-inactivating
Protein from Cucurbitaceae

林毅陈国强吴祖建林奇英** 谢联辉

2004, 12(1): 8-12 | doi: | Full text (HTML) (1 KB) |

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质粒营救法和TAIL-PCR法获得水稻 T-DNA旁邻序列的效率比较

Comparison of the Efficiency Between Plasmid Rescue and TAIL-PCR Methods
for Obtaining Flanking Sequence of T-DNA Tag Insertion in Rice Genome

张帆金维正陈双燕吴运荣刘非燕吴平*

2004, 12(1): 13-18 | doi: | Full text (HTML) (1 KB) |

PDF (301 KB) ( 342 )

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Bt 转基因抗虫恢复系选育及其杂种纯度快速鉴定

Breeding of Insect-resistant Bt Transgenic Restorer Line
and Its Hybrid Purity Determination

沈圣泉吴殿星夏英武舒庆尧

2004, 12(1): 19-23 | doi: | Full text (HTML) (1 KB) |

PDF (265 KB) ( 200 )

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结球白菜结球前期基因表达序列标签（EST）分析

Expressed Sequence Tags (EST) Analysis of the Heading Leaf of Chinese Cabbage (Brassica rapa L. ssp. pekinensis ) at the Early Heading Stage

高瑞娟1,2 戴大鹏1,2 马荣才1,2 ** 曹鸣庆1,2 晏月明2 王亚东3 任世军3 郭新宇4

2004, 12(1): 24-29 | doi: | Full text (HTML) (1 KB) |

PDF (343 KB) ( 283 )

摘要

水稻巯基蛋白酶抑制剂基因(OCI )转化甘薯获得转基因植株

Regeneration of Sweetpotato Transgenic Plants with Oryzacystatin-Ⅰ （OCI ）Gene

蒋盛军刘庆昌** 翟红邬丽莎王玉萍

2004, 12(1): 34-37 | doi: | Full text (HTML) (1 KB) |

PDF (370 KB) ( 270 )

摘要

盐胁迫对番茄幼苗中乙烯信号转导途径一些基因表达的影响

Influence of Salt Stress on Expression of Some Genes Involving
in Ethylene Singaling Pathway in Tomato Seedling

石英徐晶宇蔚变云朱本忠罗云波**

2004, 12(1): 30-33 | doi: | Full text (HTML) (1 KB) |

PDF (279 KB) ( 313 )

摘要

水稻长穗颈高秆突变体协青早eB1的遗传分析及其eui1(t) 定位

Genetic Analysis for Uppermost Internode Elongation in indica Rice Xieqingzao eB1 and Its Mapping of euil (t ) Gene

马洪丽1 张书标1 卢勤1 方宣钧2 杨蜀岚1 杨仁崔1**

2004, 12(1): 43-47 | doi: | Full text (HTML) (1 KB) |

PDF (286 KB) ( 304 )

摘要

微管蛋白荧光探针的制备及其在蚕豆叶片保卫细胞中的组装

Preparation of Rhodamine-labelled Tubulin and Its Reassembly in
Guard Cells of Leaves (Vicia faba L.)

于荣1, 2 袁明2 王学臣2**

2004, 12(1): 48-51 | doi: | Full text (HTML) (1 KB) |

PDF (246 KB) ( 274 )

摘要

半定量RT-PCR法评定不同生长阶段猪抗菌肽pr-39基因表达差异

Antibacterial Peptide pr-39 Gene Expression in Different Growth
Stages of Pig Evaluated by Semi-quantitative RT-PCR Method

汪以真林文学许梓荣

2004, 12(1): 52-55 | doi: | Full text (HTML) (1 KB) |

PDF (321 KB) ( 363 )

摘要

肌抑素Myostatin突变体Pro区域的克隆、表达和活性

Cloning and Expression of Pro Domain of Mutation of Myostatin
and Its Biochemical Activity

杨兴元杨树洪侯健陈永福 **

2004, 12(1): 56-60 | doi: | Full text (HTML) (1 KB) |

PDF (250 KB) ( 249 )

摘要

小麦中一类依赖于NAD的细胞质型苹果酸脱氢酶cDNA的克隆和进化树分析

Cloning and Evolutionary Analysis of a Partial
Cytosolic Malate Dehydrogenase cDNA from Wheat

蔺占兵徐洋马庆虎** 丁郁

2004, 12(1): 38-42 | doi: | Full text (HTML) (1 KB) |

PDF (327 KB) ( 288 )

摘要

乳源活性肽对早期断奶仔猪胃泌素mRNA表达的影响

Effect of Bioactive Peptides Derived from Casein on mRNA Express of
Gastrin in Early Weaning Piglet

张源淑邹思湘赵茹茜陈伟华

2004, 12(1): 61-65 | doi: | Full text (HTML) (1 KB) |

PDF (286 KB) ( 294 )

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CpG 寡脱氧核苷酸免疫刺激活性的种属特异性

Heterogeneity in the Response of Different Animals to Specific
CpG Oligodeoxynucleotides

李光富1** 朱鸿飞2 马艳琴1 朱建中1 邵国清3 卢春1 张兆松1

2004, 12(1): 66-70 | doi: | Full text (HTML) (1 KB) |

PDF (314 KB) ( 418 )

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猪瘟兔化弱毒株E2蛋白A/D抗原区基因的原核表达

Prokaryotic Expressing of the Gene Coding the Hog
Cholear Lapinised Virus E2 A/D Antigenic Domains

苏小运1 许立华1，2 徐学清1 杨仁全1 陈德胜1 陈溥言1**

2004, 12(1): 71-75 | doi: | Full text (HTML) (1 KB) |

PDF (357 KB) ( 237 )

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转cry1Ac/CpTI 双价抗虫水稻Cry1Ac杀虫蛋白的表达特性
及其对二化螟的毒杀效果

Expression of Cry1Ac Protein in cryAc/CpTI Transgenic Rice
and Its Resistance in Different Stages to Chilo suppressalis

赵红盈1，2 张永军1 吴孔明1** 赵奎军2 彭于发1 郭予元1

2004, 12(1): 76-79 | doi: | Full text (HTML) (1 KB) |

PDF (284 KB) ( 202 )

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柑桔黄龙病病原16S rDNA克隆、测序及实时荧光
PCR检测方法的建立

Cloning and Sequencing of Citrus Huanglongbing Pathogen 16S rDNA and Its
Detection by Real-time Fluorescent PCR

廖晓兰1 朱水芳2** 赵文军2 罗宽1 漆艳香 1 陈红运2 何昆1 朱晓湘1

2004, 12(1): 80-85 | doi: | Full text (HTML) (1 KB) |

PDF (422 KB) ( 591 )

摘要

小麦抗叶锈基因Lr37 ISSR分子标记

ISSR Molecular Markers for the Leaf Rust Resistance Gene Lr37 in Wheat

张立荣徐大庆杨文香刘大群

2004, 12(1): 86-89 | doi: | Full text (HTML) (1 KB) |

PDF (313 KB) ( 178 )

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福建马铃薯A病毒的分子鉴定及检测技术

Molecular Identification and Detection Method of Potato Virus A

吴兴泉陈士华魏广彪吴祖建谢联辉**

2004, 12(1): 90-95 | doi: | Full text (HTML) (1 KB) |

PDF (313 KB) ( 265 )

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绿色木霉的原生质体制备与转化条件

Isolation and Transformation of Trichoderma viride Protoplasts

刘士旺1 王政逸1 郭泽建1.2**

2004, 12(1): 96-101 | doi: | Full text (HTML) (1 KB) |

PDF (366 KB) ( 311 )

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综述

猪神经内分泌生长轴各因子及相关基因的研究进展

Studies on Genes and Factors in Neuro-endocrine Growth Axis in Pig

刘德武杨关福张细权

2004, 12(1): 109-115 | doi: | Full text (HTML) (1 KB) |

PDF (303 KB) ( 486 )

摘要

植物芪类次生代谢物的功能、合成调控及基因工程研究进展

Advances of Biological Function, Regulatory Mechanism of Biosynthesis and Genetic Engineering of Stilbenes in Plant

何水林1** 郑金贵2 林明2 王燕华2

2004, 12(1): 102-108 | doi: | Full text (HTML) (1 KB) |

PDF (435 KB) ( 483 )

摘要