农业生物技术学报

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农业生物技术学报 2003年 11卷 5期刊出日期：2003-09-20

专家论坛

牛胚胎干细胞建系研究进展及存在问题

Progress and Problems on Establishing Bovine Embryonic Stem Cell Lines

窦忠英徐小明华进联

2003, 11(5): 439-443 | doi: | Full text (HTML) (1 KB) |

PDF (219 KB) ( 370 )

摘要

研究论文

转基因水稻中ThEn-42基因的稳定遗传及其抗病性的提高*

Genetic Stability and Enhanced Resistance of Transgenic Rice with ThEn-42 Gene

刘梅1,2 覃宏涛1,2 孙宗修2 徐同1**

2003, 11(5): 444-449 | doi: | Full text (HTML) (1 KB) |

PDF (429 KB) ( 133 )

摘要

利用RAPD和SSR标记分析陆地棉种质资源的遗传多样性*

Analysis of Genetic Diversity in Upland Cotton (Gossypium hirsutum L.) Cultivars

朱龙付张献龙** 聂以春

2003, 11(5): 450-455 | doi: | Full text (HTML) (1 KB) |

PDF (252 KB) ( 278 )

摘要

用巢式和半巢式PCR检测转基因大豆Roundup Ready
及其深加工食品*

Detecting Genetically Modified Soybean Roundup Ready Ingredient in Foodstuffs
by Nested PCR and Semi-nested PCR

黄昆仑罗云波**

2003, 11(5): 461-466 | doi: | Full text (HTML) (1 KB) |

PDF (358 KB) ( 546 )

摘要

转基因大豆加工产品的定性PCR检测*

Qualitative Analysis of the Processed Genetically Modified Soybean
Products by PCR-based Methods

郑文杰1 刘烜1 刘伟2 唐丹舟1 何霖1 张宏伟1

2003, 11(5): 467-471 | doi: | Full text (HTML) (1 KB) |

PDF (276 KB) ( 880 )

摘要

结球白菜结球莲座期和包心期叶片mRNA差异表达研究*

mRNA Differential Display Analysis at Rosette and Folding Stages of Chinese Cabbage (Brassica campestris L.ssp.pekinensis )

郭晶心1 周乃元1，2 马荣才1，2 曹鸣庆1，2**

2003, 11(5): 456-460 | doi: | Full text (HTML) (1 KB) |

PDF (310 KB) ( 226 )

摘要

转Cry1Ac和Cry1A+CpTI基因棉花粉对柞蚕生长发育影响的评价*

Evaluation of Impact of Pollen Grains of Cry1Ac and Cry1A+CpTI Transgenic Cotton on the Growth and Development of Chinese
Tussah Silkworm（Antheraea pernyi）

李文东1，2 吴孔明1** 王小奇2 郭予元1

2003, 11(5): 488-493 | doi: | Full text (HTML) (1 KB) |

PDF (383 KB) ( 290 )

摘要

牛腔前卵泡入培直径及每孔培养卵泡数对其体外发育的影响*

Effects of Pre-culture Diameter of Preantral Follicles and the
Number of Follicles in Each Well on In Vitro Development of Preantral Follicles

李海周虚** 高志花安铁洙

2003, 11(5): 500-505 | doi: | Full text (HTML) (1 KB) |

PDF (354 KB) ( 292 )

摘要

NCSU23、SOF及氨基酸对猪孤雌激活胚胎体外发育的影响*

Effects of NCSU23, SOF and Amino Acids on In Vitro
Development of Porcine Parthenogenetic Embryos

吴中红邢凤英刘国世曾申明** 朱士恩张忠诚

2003, 11(5): 511-515 | doi: | Full text (HTML) (1 KB) |

PDF (211 KB) ( 213 )

摘要

试管微嫁接早期鉴定小金海棠与苹果品种的亲和性*

Early Testing of Grafting Compatibilities Between Malus xiaojinensis and Two Apple Cultivars by Micrografting In Vitro

程玉琴韩振海** 许雪峰王文珺

2003, 11(5): 472-476 | doi: | Full text (HTML) (1 KB) |

PDF (343 KB) ( 246 )

摘要

华北地区转Cry1A+CpTI和Cry1A基因棉棉田害虫
和天敌昆虫的群落结构*

Effects of Transgenic Cottons Carrying Cry1A+CpTI and Cry1A Genes on the Structures and Composition of Pest and Beneficial
Arthropod Communities in Cotton Field in North China

郦卫弟1，2 吴孔明1 陈学新2** 封红强1 徐广1 郭予元1

2003, 11(5): 494-499 | doi: | Full text (HTML) (1 KB) |

PDF (549 KB) ( 371 )

摘要

侵染云南白肋烟的中国番茄黄化曲叶病毒及伴随卫星
DNA分子的基因组特征*

Genomic Characterization of DNA-A and Associated Satellite DNA Molecule of an Isolate of Tomato Yellow Leaf Curl China Virus Infecting Nicotiana tabacum White Burley in Yunnan*

李桂新范三微李正和谢艳周雪平**

2003, 11(5): 525-530 | doi: | Full text (HTML) (1 KB) |

PDF (397 KB) ( 363 )

摘要

樟疫多聚半乳糖醛酸酶Pcpg 1、Pcpg 2
和Pcpg 4基因的克隆、测序及其遗传转化*

Cloning, Sequencing and Genetic Transformation of Pcpg (Phytophthora cinnamomi polygalacturonase)1 , Pcpg 2 and Pcpg 4 Genes

巩振辉1 Arvid Gotesson2 David A. Jones2

2003, 11(5): 477-482 | doi: | Full text (HTML) (1 KB) |

PDF (717 KB) ( 216 )

摘要

家蚕核型多角体病毒埃及株p10基因的克隆及结构特征分析*

Nucleotide Sequence and Characterization of p10 Gene of Bombyx mori
Nuclear Polyhedrosis Virus Egyptian Strain

姜丽华1，2 钟万芳1 蔡平钟1，3** 徐兴耀2 阎文昭1，3** 吴杰1，3

2003, 11(5): 483-487 | doi: | Full text (HTML) (1 KB) |

PDF (309 KB) ( 237 )

摘要

血管活性肠肽与乙肝病毒核心抗原的融合表达、鉴定及其纯化*

Expression, Verification and Purification of Fusion Protein of Vasoactive Intestinal Polypeptide and Hepatitis B Virus Core Antigen

黄运茂施振旦 ** 刘颖毕英佐刘丽

2003, 11(5): 506-510 | doi: | Full text (HTML) (1 KB) |

PDF (354 KB) ( 302 )

摘要

无花果曲酶植酸酶基因phyA的克隆、序列分析及表达*

Cloning, Sequence Analysis and Expression of the
Phytase phyA Gene from Aspergillus ficuum

李桂兰1,2 祝建洪1 孙建1 吴作为3 王隆飞1 晏晶1 陈功1 姜洪州1 李明刚1**

2003, 11(5): 520-524 | doi: | Full text (HTML) (1 KB) |

PDF (429 KB) ( 228 )

摘要

苏云金芽孢杆菌Ly30株cry1Ac基因的克隆及表达*

Cloning and Expression of cry1Ac Gene from A Novel
Bacillus thuringiensis Ly30 Strain

姚江1 张杰1 宋福平1 李长友1 刘旭光1 黄大昉1, 2**

2003, 11(5): 516-519 | doi: | Full text (HTML) (1 KB) |

PDF (418 KB) ( 321 )

摘要

综述

十字花科作物细胞质雄性不育的分子机理*

The Molecular aspects of Cytoplasmic Male Sterility in Cruciferous Crops

张明方杨景华陈竹君

2003, 11(5): 538-544 | doi: | Full text (HTML) (1 KB) |

PDF (486 KB) ( 230 )

摘要

植物油体表达体系的研究进展*

Progress in the Establishment of a Plant Seed Oil Body Expression System

刘昱辉贾士荣**

2003, 11(5): 531-537 | doi: | Full text (HTML) (1 KB) |

PDF (362 KB) ( 543 )

摘要

研究简报

DDRT-PCR与反向Northern杂交技术结合分离马铃薯mRNA差异表达片段*

Isolation and Identification of Differentially Expressed mRNA Species by DDRT-PCR
Combined with Reverse Northern Hybridization in Potato

田振东1 柳俊2 谢从华1** 宋波涛1

2003, 11(5): 545-546 | doi: | Full text (HTML) (1 KB) |

PDF (154 KB) ( 284 )

摘要