Isolation and Expression of Myostatin (MSTN) Genes, and Their Polymorphism Correlations with Body Formand Average Daily Gain in Cyprinus carpio var. jian
Abstract：Myostatin(MSTN) is found to be a negative regulator of muscle growth recently, which belongs to transforming growth factor-β(TGF-β) family. Four jlMSTNs genes(jlMSTN1a, jlMSTN1b, jlMSTN2a and jlMSTN2b), with the same genetic structure, were isolated from genome of Cyprinus carpio var. jian using PCR. Homology analysis showed that jlMSTN1a, jlMSTN1b and jlMSTN2a, jlMSTN2b belonged to fish MSTN1 and MSTN2, respectively. The similarities of ORFs between the two paralogs were 96% and 94%, but there were length or sequence differences occurred to the introns. Two introns of jlMSTN2a were obviously longer than that of jlMSTN2b , 1 384 bp, 1 763 bp and 879 bp, 835 bp, respectively. The different expression levels in different tissues and the SNP loci indicated that jlMSTN1s standed higher selection stress than that of jlMSTN2s; jlMSTN2a standed higher selection stress than that of jlMSTN2b. This study also identified that 2 SNP loci associated with body form and ADG (average daily gain) separately, which can be used as markers of assistant breeding.
