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2025年4月6日 星期日
  2018, Vol. 26 Issue (2): 272-283    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
兴义鸭MEF2D基因的多态性及组织差异表达研究
师新彩1,赵忠海1,李辉1,卜小雁2,易恒洁1,陈林1
1. 贵州大学
2. 贵州大学 动物科学学院
Polymorphism and Tissue Differential Expression of MEF2D Gene in Xingyi Duck (Anas platyrhynchos domestica)
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摘要 肌细胞增强因子2D(myocyte enhancer factor 2D, MEF2D)是属于肌细胞增强因子2(myocyte enhancer factor 2, MEF2)基因家族一员,被认为与骨骼肌生长发育有关。为研究MEF2D基因的多态性对兴义鸭(Anas platyrhynchos domestica)屠宰性状的影响,本研究利用PCR扩增结合测序的方法对90日龄兴义鸭MEF2D基因外显子区域进行SNP位点筛选,同时运用qRT-PCR对0~150日龄兴义鸭不同组织中该基因mRNA表达规律进行探究。结果显示,在MEF2D基因外显子区域共发现6个SNPs,均为同义突变;群体遗传学分析显示,6个多态位点均表现为中度多态(0.25<PIC<0.5),经χ2检验表明兴义鸭群体在这6个多态位点均处于Hardy-weinberg平衡状态(P>0.05);各SNPs与屠宰性状关联分析发现,T16236C位点的不同基因型在活重和屠体重存在显著差异(P<0.05),A16305C和A21071G位点对多项屠宰指标存在显著或极显著影响(P<0.05或P<0.01),G16359C位点的CC基因型个体半净膛重、全净膛重显著高于GG基因型个体(P<0.05),其余SNPs对屠宰性状无显著影响(P>0.05);单倍型分析结果表明,兴义鸭CGCCCG单倍型的半净膛重和半净膛率显著或极显著高于其他单倍型(P<0.05或P<0.01);TACCCA单倍型的腿肌重显著高于其他单倍型(P<0.05);TATAGA单倍型的全净膛率显著高于其他单倍型(P<0.05);qRT-PCR结果显示,在10个组织中均检测到了MEF2D基因的表达,说明该基因的表达具有广谱性,且公母差异显著,母鸭腿肌中的表达量最高,心、肝脏、肺脏、肾脏、十二指肠、大脑、胸肌、腺胃中的表达量较高,而公鸭心肌、肝脏中的表达量最高,十二指肠、大脑、胸肌、腿肌、腺胃中的表达量较高。研究结果可为兴义鸭屠宰性状的分子标记辅助选育提供参考,同时为鸭MEF2D基因结构功能的进一步研究提供理论基础资料。
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师新彩
赵忠海
李辉
卜小雁
易恒洁
陈林
关键词 MEF2D基因多态性屠宰性状组织表达兴义鸭    
Abstract:Myocyte enhancer factor 2D (MEF2D) is a member of the myocyte enhancer factor 2 (MEF2), a supergene family, and thought to be related to skeletal muscle growth and development. To investigate the effects of polymorphism of MEF2D gene on slaughter traits of Xingyi ducks (Anas platyrhynchos domestica), this study used PCR and DNA sequencing methods to find SNP loci and analyzed their correlation with slaughter traits. Meanwhile, the expression profile of MEF2D gene was investigated by qRT-PCR of different stages Xingyi ducks. Finally, the results showed 6 SNPs in exons, respectively, C3894T, G3957A, T16236C, A16305C, G16359C and A21071G. All of the six sites were synonymous mutation, and those did not cause a change of amino acid encoding. By population genetics analysis, the results showed that 6 loci were at moderately polymorphic status (0.25<PIC<0.5). The χ2 tests showed that the loci were all in Hardy-Weinberg equilibrium status (P>0.05). Analysis of matching chain disequilibrium and haplotype analysis show that there was a strong chain of balance between the six loci. Analysis of association of polymorphism with slaughter traits at all mutation loci showed that T16236C locus and A16305C locus were significant effects on live weight, carcass weight, semi-eviscerated weight, eviscerated weight, and breast muscle weight (P<0.05 or P<0.01); G16359C locus was significant effects on semi-eviscerated weight and eviscerated weight (P<0.05); A21071G locus was significant effects on carcass weight, eviscerated weight, leg muscle weight, dressing percentage and eviscerated weight rate (P<0.05 or P<0.01). Seven haplotype combinations were found in 52 Xingyi ducks, the CGCCCG haplotype have significant effects on semi-eviscerated weight and semi-eviscerated weight rate (P<0.05 or P<0.01); TACCCA (TATAGA) haplotype have significant effects on leg muscle weight (eviscerated weight rate) (P < 0.05). The results of qRT-PCR showed that MEF2D was expressed in all 10 involved tissues, which illustrated that the expression of this gene was of broad spectrum, and exists significant difference between ducks and drakes. In all of the duck tissues, the highest expression of leg muscle was the highest, the expression level of heart, liver, lung, kidney, brain, muscle duodenum and glandular stomach were relatively higher. And in all of the drake issues, the expression level of cardiac muscle and liver were the highest, duodenum, brain, muscle, leg muscle and glands in the stomach were relatively high. The results suggested that the variation trend of mRNA expression level of MEF2D in most issues of ducks at different age stages was first increased and then decreased and then increased; drakes showed the trend of decrease- increase- decrease. The results suggested that four SNPs and three haplotype combinations might have potential effects on slaughter traits in the above mentioned duck populations and could be used for marker-assisted selection. This study could provide a theoretical basis for the follow-up analysis of structure and function of MEF2D gene in ducks, further enrich the research results of MEF2D.
Key wordsMyocyte enhancer factor 2D (MEF2D)    Polymorphism    Slaughter traits    Tissue expression    Xingyi ducks
收稿日期: 2017-06-30      出版日期: 2018-02-04
ZTFLH:  S834  
  S834+.83  
基金资助:教育部科学技术研究重点项目;贵州省科技合作计划项目
通讯作者: 李辉     E-mail: ellenlihui@sina.cn
引用本文:   
师新彩 赵忠海 李辉 卜小雁 易恒洁 陈林. 兴义鸭MEF2D基因的多态性及组织差异表达研究[J]. , 2018, 26(2): 272-283.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2018/V26/I2/272
 
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