Abstract：Abstract Diacylglycerol acyltransferase 1(DGAT1), considered as an important candidate gene for milk fat traits, plays key roles in triglycerides synthesis. The Bos grunniens milk has rich nutrition and higher milk fat. However, there are few studies on the genetic mechanism of dairy quality traits, because it is restricted by distribution, local environment, economy and production condition. In the present study, the tissue distribution of Bos grunniens DGAT1 mRNA and the genotype polymorphisms in 3'-untranslated region (3' UTR) and exon 8 of yak DGAT1 gene were detected by quantitative real-time PCR (qRT-PCR) and single strand conformation polymorphism (SSCP), and the associations of different genotypes and alleles with milk quality traits of Gannan yak were analyzed. The results indicated that the basal expression level of DGAT1 were relatively high in mammary gland, longissimus dorsi, heart and subcutaneous fat, relatively moderate in kidney, small intestine, large intestine, but low in spleen. At the c.*197 T>C locus, animals with genotype FF had higher milk fat rate than those with EE and EF genotypes (P＜0.05). And individuals with allele E had lower milk fat rate and total solids percentage than those with missing allele E (P＜0.01 or P＜0.05). The double-bases mutation c.691-c.692 AA>GC(K232A) was found in Gannan yak and Tianzhu white yak, the allele K was predominant, but those mutations were not detected in Qinghai yak and Wild-blood yak. The presence of allele K was beneficial to increasing the milk fat rate. The results suggested that 3'-untranslated region of yak DGAT1 gene may have a certainly genetic effect on milk quality traits. These results enrich the molecular genetic data for dairy quality traits of yak.
黄友鹰,程良.1997.中国牦牛奶制品工艺学[M].四川科学技术出版社,成都.pp.21-29.刘贤慧,常玲玲,吴正常,等.2013.荷斯坦牛二酰基甘油酰基转移酶1基因外显子8的单核苷酸多态性位点对蛋白质结构和功能的影响及其进化分析[J].中国畜牧兽医,40(7):14-17. 芦春艳.2012.奶牛乳腺上皮细胞系的建立及DGAT1基因功能的验证[D].硕士学位论文,吉林大学.导师:赵志辉.pp.53-55.田璐,岳文斌,黄萌,等.2010.不同肉牛品种中乙酰辅酶A:二酰甘油酰基转移酶基因(DGAT1 )遗传多态性及对胴体性状的影响[J].农业生物技术学报,18(6),1108-1114.王国富,吴慧光,孙国权,等.2012.中国西门塔尔牛DGAT1和DGAT2基因组织表达谱及其在脂肪组织中的表达与背膘厚的关联分析[J].华北农学报, 27(5):60-64.王海震,王莹,刘定干.2008.真核生物mRNA 3’非翻译区的功能[J]. 生物化学与生物物理进展,35(09): 980-985.徐秀容,高雪,许尚忠,等.2006.牛DGAT1基因K232A取代对3个品种奶牛群体部分经济性状的影响(英文)[J].西北农林科技大学学报自然科学版, 34(9):6-10.昝林森,邹荣婕,田万强,等.2007.中国荷斯坦奶牛DGAT1基因3’-UTR的PCR-SSCP分析[J].西北农业学报,16(4):10-13.邹荣婕.2007.中国荷斯坦奶牛DGAT1、LOX-1基因多态性与产奶性状之间的关系研究[D].硕士学位论文,西北农林科技大学.导师:昝林森.pp.48-50.Cases S, Smith S J, Zheng Y W, et al.1998.Identification of a gene encoding an acyl-CoA:diacylglycerol acyltransferase, a key enzyme in triacylglycerol synthesis.[J]. Proceedings of the National Academy of Sciences of the United States of America, 95(22):13018-13023.Coudreau S K, Tounian P, Bonhomme G, et al.2003. Role of the DGAT Gene C79T Single Nucleotide Polymorphism in French Obese Subjects[J]. Obesity Research, 10(11):1163-1167.Fürbass R,Winter A,Fries R,et.al.2006.Alleles of the bovine DGAT1 variable number of tandem repeat associated with a milk fat QTL at chromosome 14 can stimulate gene expression[J]. Physiol Genomics,25:116-120. Grisart B, Coppieters W, Farnir F, et al.2002. Positional candidate cloning of a QTL in dairy cattle: Identification of a missense mutation in the bovine DGAT1 gene with major effect on milk yield and composition[J]. Genome Research, 12(2):222-231.Harris Charles A,Haas Joel T,Streeper Ryan S,et al.2011.DGAT enzymes are required for triacylglycerol synthesis and lipid droplets in adipocytes.[J].Journal of Lipid Research,52(4):657-667.Hu J,Zhou H,Smyth Aet al.2010.Polymorphism of the bovine ADRB3 gene.Molecular Biology Reports, (37):3389-3392.Jeong J, Kwon E G, Im S K, et al. 2012.Expression of fat deposition and fat removal genes is associated with intramuscular fat content in longissimus dorsi muscle of Korean cattle steers[J]. Journal of Animal Science,90(6):2044-2053.Liu W, Yue Y, Lin Y Q, et al.2011. Yak DGAT1 gene: Cloning, tissue expression profile, splicing and polymorphism analysis[J]. Livestock Science, 142(1–3):264-269.Mohammed S A, Rahamtalla S A,Ahmed S S, et al. 2015.DGAT1 gene in dairy cattle[J].Global Journal of Animal Scientific Research ,3(1):191-198.Naslund J, Fikse W F, Pielberg G R, et al.2008. Frequency and effect of the bovine acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1) K232A polymorphism in Swedish dairy cattle. Journal of Dairy Science, 91: 2127-2134.Schennink A, Stoop W M, Visker M H P W, et al.2007.DGAT1 unterlies large genetic variation in milk fat composition of dairy cows[J]. Animal Genetics, 2007, 38(5): 467-473Signorelli F, Orru L Napolitano F, Matteis G D, et al. 2009.Exploring polymorphisms and effects on milk traits of the DGAT1, SCD1 and GHR genes in four cattle breeds[J]. Livestock Science, 125(1):74-79.Winter A, Kramer W, Werner F, et al.2002.Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA :diacylglycerol acyltransferase (DGAT1)with variation at a quantitative trait locus for milk fat content [J].Proceedings of the National Academy of Sciences,99(14):9300-9305.Zhang L, Sun C, Ye X, et al. 2014.Characterization of four heat-shock protein genes from Nile tilapia (Oreochromis niloticus) and demonstration of the inducible transcriptional activity of Hsp70 promoter.[J]. Fish Physiology and Biochemistry, 40(1):221-233.
