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2025年4月4日 星期五
  2017, Vol. 25 Issue (9): 1443-1450    
  研究论文与报告 本期目录 | 过刊浏览 | 高级检索 |
牦牛DGAT1基因组织表达及其突变与乳品质性状的关联分析
高小莉1,赵志东2,石斌刚3,谢建鹏4,刘秀5,马桂琳1,杨耀3,胡江6,郭淑珍2
1. 甘肃农业大学
2.
3. 甘肃农业大学 动物科学技术学院
4. 甘肃省草食动物生物技术重点实验室
5. 甘肃农业大学甘肃省草食动物生物技术重点实验室
6. 甘肃农业大学动物科学技术学院/甘肃省草食动物生物技术重点实验室
Tissue Expression and Polymorphisms of DGAT1 Gene and Their Association with Milk Quality Traits in Yak (Bos grunniens)
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摘要 摘 要 二酰基甘油酰基转移酶1(diacylglycerol acyltransferase 1, DGAT1)作为乳脂性状的重要候选基因,是合成甘油三酯的关键酶。牦牛(Bos grunniens)乳营养丰富,乳脂含量较高;但受分布局域环境、经济及生产条件制约,对其乳品质性状遗传机理研究较少。本研究采用qRT-PCR及单链构象多态性(single strand conformation polymorphism, SSCP)方法,检测牦牛DGAT1基因组织表达,以及3'-UTR和exon8区域多态位点,分析不同基因型及等位基因与甘南牦牛乳品质性状的相关性。结果表明,甘南牦牛DGAT1基因在乳腺、背最长肌、心脏和皮下脂肪高度表达,在肾脏、小肠和大肠中度表达,在脾脏表达最低;4个牦牛群体中DGAT1基因3'-UTR均检测到c.*197 T>C的突变,基因型FF个体乳脂率显著高于基因型EE和EF(P<0.05),携带等位基因E的个体乳脂率和总固体物质百分含量显著低于未携带者(P<0.01或P<0.05);甘南牦牛和天祝白牦牛exon 8区检测到c.691-c.692 AA>GC(K232A)的双碱基突变,等位基因K为优势等位基因,青海牦牛及野血牦牛该区域内未检测到多态性,推测等位基因K的存在有利于增加牦牛乳脂率。研究结果表明,DGAT1基因3'-UTR区域对牦牛乳质性状有一定的基因效应。本研究可为牦牛乳品质性状分子遗传研究提供基础数据。
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作者相关文章
高小莉
赵志东
石斌刚
谢建鹏
刘秀
马桂琳
杨耀
胡江
郭淑珍
关键词 牦牛二酰基甘油酰基转移酶1基因(DGAT1)组织表达多态性乳品质性状    
Abstract:Abstract Diacylglycerol acyltransferase 1(DGAT1), considered as an important candidate gene for milk fat traits, plays key roles in triglycerides synthesis. The Bos grunniens milk has rich nutrition and higher milk fat. However, there are few studies on the genetic mechanism of dairy quality traits, because it is restricted by distribution, local environment, economy and production condition. In the present study, the tissue distribution of Bos grunniens DGAT1 mRNA and the genotype polymorphisms in 3'-untranslated region (3' UTR) and exon 8 of yak DGAT1 gene were detected by quantitative real-time PCR (qRT-PCR) and single strand conformation polymorphism (SSCP), and the associations of different genotypes and alleles with milk quality traits of Gannan yak were analyzed. The results indicated that the basal expression level of DGAT1 were relatively high in mammary gland, longissimus dorsi, heart and subcutaneous fat, relatively moderate in kidney, small intestine, large intestine, but low in spleen. At the c.*197 T>C locus, animals with genotype FF had higher milk fat rate than those with EE and EF genotypes (P<0.05). And individuals with allele E had lower milk fat rate and total solids percentage than those with missing allele E (P<0.01 or P<0.05). The double-bases mutation c.691-c.692 AA>GC(K232A) was found in Gannan yak and Tianzhu white yak, the allele K was predominant, but those mutations were not detected in Qinghai yak and Wild-blood yak. The presence of allele K was beneficial to increasing the milk fat rate. The results suggested that 3'-untranslated region of yak DGAT1 gene may have a certainly genetic effect on milk quality traits. These results enrich the molecular genetic data for dairy quality traits of yak.
Key wordsYak    Diacylglycerol acyltransferase 1 gene (DGAT1)    Tissue expression    Polymorphism    Milk quality traits
收稿日期: 2017-04-28      出版日期: 2017-08-06
基金资助:甘肃省农业生物技术研究与应用开发项目;国家自然科学基金项目;甘肃省杰出青年科学基金项目
通讯作者: 胡江     E-mail: huj@gsau.edu.cn
引用本文:   
高小莉 赵志东 石斌刚 谢建鹏 刘秀 马桂琳 杨耀 胡江 郭淑珍. 牦牛DGAT1基因组织表达及其突变与乳品质性状的关联分析[J]. , 2017, 25(9): 1443-1450.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/     或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2017/V25/I9/1443
 
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