Microsatellite Analysis of Landrace Rice Core Collection in Yunnan, China
Zhang Hongliang1 Li Zichao1** Liao Dengqun1 Liu Xia1,3 Zeng Yawen2 Shen Shiquan2 Mu Ping1 Yang Zhongyi2 Wang Xiangkun1
(1. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China; 2. Crop Germplasm Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China; 3.College of Agronomics, Xinjiang Agricultural University, Urumchi 830052, China)
Abstract：Abstract: The distribution of genetic diversity between Oryza sativa L. ssp. indica and O. sativa L. ssp. japonica covered different ecological zones in Yunnan was Studied, and the specific markers of indica /japonica subspecies, paddy/upland rice and different ecological zones were screened, using 36 microsatellite primers and 113 accessions in Yunnan landrace rice core collection. The results showed that the genetic diversity of japonica was higher than that of indica , and the ecological zone with the highest and smallest genetic diversity lay in Southeast and Northeast of Yunnan respectively. This distribution was consistent with the results of the studies on whole Yunnan rice resources and core collection at morphological and isozyme levels. In addition, the results showed that, among 416 markers, there were 6indica /japonica-specific markers, 15 specific markers in paddy/upland and 3 specific markers in different ecological zones. So the conclusion was primarily that the landrace rice core collection in Yunnan genetically represented the whole landrace rice resources in Yunnan, the center of genetic diversity at DNA level lay in Southeast of Yunnan, and the DNA differentiation between indica and japonica was small. And microsatellite marker was a useful tool to study the genetic diversity, classification and ecotype of germplasm resources and their core collection.
