联系我们 加入收藏
 
年期检索 高级检索
33
2025年4月3日 星期四
  研究论文 本期目录 | 过刊浏览 | 高级检索 |
猪OPN基因与繁殖性状的关联分析
罗仍卓么 王立贤 孙世铎
中国农业科学院北京畜牧兽医研究所401室;西北农林科技大学动物科技学院 中国农业科学院北京畜牧兽医研究所 西北农林科技大学动物科技学院
Genetic Polymorphism of OPN Gene and Correlation with Reproductive Traits in Pig
Zhuo-Ma LUOREN Li-xian WANG Shi-duo SUN
全文: PDF (0 KB)  HTML (1 KB) 
输出: BibTeX | EndNote (RIS)      
摘要 摘要:OPN(osteopontin)基因是近年来在猪的繁殖性状上发现的一个候选基因,本试验以长白猪,大白猪和北京黑猪为研究对象,研究OPN基因多态性与猪的繁殖性状之间的关联。经特异性产物的扩增,结合测序发现,OPN基因的第6内含子存在305bp的缺失突变,从而产生多态。在长白猪和大白猪中发现了AA、AB和BB 共3种基因型,在2个群体中处于中度多态,且已达到Hardy-Weinberg平衡状态。而在北京黑猪群体中没有多态,只有BB基因型。利用SAS 8.0 软件采用最小二乘法拟合线性模型,将不同基因型与总产仔数(TNB)、产活仔数(NBA)和初生重(WB)进行了关联分析,结果表明:长白猪初产母猪AA型个体的TNB、NBA显著的高于AB和BB型个体(P<0.05),经产母猪AB型个体的TNB和NBA显著的高于AA型与BB型个体(P<0.05),A等位基因对TNB、NBA和WB均表现为正效应。在大白猪中,不论是初产母猪还是经产母猪,其BB和AB型个体的TNB、NBA高于AA型个体,但其差异不显著(P>0.05),B等位基因对TNB、NBA都表现为正效应。
服务
把本文推荐给朋友
加入我的书架
加入引用管理器
E-mail Alert
RSS
作者相关文章
罗仍卓么王立贤孙世铎
关键词 OPN基因多态性繁殖性状关联分析    
Abstract:Abstract:Osteopontin gene was regard as a candidate gene for reproductive traits of pig in recent years, in this study, genetic polymorphism of OPN gene and correlation with reproductive traits was studied in Landrace pig, Large White pig and Beijing Black Pig. The polymorphism was found by the PCR, which was induced by a deletion mutation of 305bp in the sixth intron of OPN gene after sequencing. AA, AB and BB genotypes were found in Landrace pig and Large White pig populations, which showed a moderate polymorphism. The statistical results of χ2-test indicated that this locus fitted Hardy-Weinberg equilibrium. While polymorphism of this locus was not found in Beijing Black pig, and the BB genotype was only present. Meanwhile, the effect of polymorphism for OPN gene on total number born (TNB), number born alive (NBA) and born weight (WB) were analyzed, the results indicated that the individuals of the first parity Landrace pig with AA genotype showed more TNB and NBA significantly than that with AB and BB genotype (P<0.05), the individual of multiparous sows with AB genotype showed more TNB and NBA significantly than that with AA and BB genotype (P<0.05), and A allele might have the positive effective on TNB, NBA and WB. While in Large White pig, the TNB and NBA of sow with BB and AB genotype were more than those with genotype AA, but the results showed no significant difference (P>0.05), and B allele might have the positive effective on TNB and NBA.
Key wordspig    OPN    polymorphism    reproductive traits    association analysis
收稿日期: 2007-08-27     
通讯作者: 王立贤   
引用本文:   
罗仍卓么 王立贤 孙世铎. 猪OPN基因与繁殖性状的关联分析[J]. , 2008, 16(3): 0-.
Zhuo-Ma LUOREN Li-xian WANG Shi-duo SUN. Genetic Polymorphism of OPN Gene and Correlation with Reproductive Traits in Pig. , 2008, 16(3): 0-.
链接本文:  
http://journal05.magtech.org.cn/Jwk_ny/CN/      或     http://journal05.magtech.org.cn/Jwk_ny/CN/Y2008/V16/I3/0
 
版权所有 © 2014 《农业生物技术学报》编辑部   京ICP备11035905号-3
地址:北京市海淀区圆明园西路2号中国农业大学生命科学楼1053室 邮编:100193
电话:010-62733684 传真:010-62731615 E-mail: nsjxb@cau.edu.cn