Association Between FTO Gene Polymorphism and Serum Lipid Metabolism Indexes in Qianbei Ma Goat (Capra hircus)
WENG Ji-Mei, LONG An-Ju, AI Jin-Xin, LUO Zheng, CAI Hui-Fen, LUO Wei-Xing*
Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education/College of Animal Science, Guizhou University, Guiyang 550025, China
Abstract:Fat mass and obesity associated gene (FTO) is an obesity susceptibility gene discovered by genome-wide scanning technology.FTO could regulate fatty acid transport and fat metabolism-related genes by transcription factors and transporters, participating in the development, maintenance and deposition of adipose tissue.Qianbei Ma goat is one of the three local varieties in Guizhou province.It is delicious and has good meat production.In present study, Qianbei Ma sheep (Capra hircus) was taken as the investigated subject, and the association between FTO gene exon polymorphism and serum physiological and biochemical indexes related to lipid metabolism was studied.SNPs were firstly screened by hybrid DNA pool combined with PCR direct sequencing technology.The genotypes of the mutation sites were directly sequenced by single sample.The different genotypes of FTO gene mutations and serum leptin, hormone sensitive lipase (HSL), adiponectin, total cholesterol, triglyceride, high density lipoprotein, low density lipoprotein, alanine aminotransferase (ALT), aspartate aminotransferase (AST) were determined by SPSS 17.0.A total of 9 SNPs were found in the tested goat population: g.C10943T, g.G109518T, g.T125300A, g.C125357T, g.G125405T, gT125417C, g.T426229C, g.C426244G, g.G426347A, where g.G109518T and g.G426347A site mutations were located in the second and ninth intron regions, respectively.Chi-square results showed that each mutation site belonged to moderate polymorphism (0.5<PIC<0.2) and did not deviate from the Hardy-Weinberg equilibrium state.There were 3 genotypes in each mutation site.Correlation analysis showed that the serum AST and ALT levels in g.T125300A and g.G426347A sites were significantly different among different genotypes (P<0.01); There were significant differences in serum AST and ALT levels between g.G125405T and g.T125417C sites (P<0.05); AST differences were significant at g.T426229C sites (P<0.05), while ALT levels were significantly different (P<0.01).Serum HSL levels were significantly different between genotypes at g.T426229C and g.G426347A (P<0.05).It is concluded that the FTO gene g.T125300A, g.G125405T, g.T125417C, g.T426229C and g.G426347A sites have a certain correlation with the lipid metabolism of Qianbei Ma goat which provide a theoretical reference for screening auxiliary selection markers of fat deposition and meat quality regulation in goats.
翁吉梅, 龙安距, 艾锦新, 罗铮, 蔡惠芬, 罗卫星. FTO基因多态性与黔北麻羊血清脂代谢指标的关联性研究[J]. 农业生物技术学报, 2019, 27(5): 875-884.
WENG Ji-Mei, LONG An-Ju, AI Jin-Xin, LUO Zheng, CAI Hui-Fen, LUO Wei-Xing. Association Between FTO Gene Polymorphism and Serum Lipid Metabolism Indexes in Qianbei Ma Goat (Capra hircus). 农业生物技术学报, 2019, 27(5): 875-884.
[1] 付言峰, 李兰, 王学敏, 等.2013.苏钟猪FTO基因的mRNA表达、克隆测序及其生物信息学分析[J].华北农学报, 28(1): 128-134. (Fu Y F, Li L, Wang X M, et al.2013.mRNA expression Suzhong porcine FTO gene, cloned and sequenced and bioinformatics analysis[J].North China Agricultural Sciences, 28(1): 128-134.) [2] 桂林生, 昝林森, 王洪宝, 等.2015.SIRT1和SIRT2基因多态性及合并基因型与秦川牛肉用性状的关联分析[J].畜牧兽医学报, 46(10): 1741-1749. (Gui L S, Yan L S, Wang H B, et al.2015.Association analysis between SIRT1 and SIRT2 gene polymorphisms and combined genotypes and traits of Qinchuan beef[J].Journal of Animal Husbandry and Veterinary Medicine, 46(10): 1741-1749.) [3] 黄英, 杨明华, 毕保良, 等.2014.乌金猪FTO基因的克隆和表达分析[J].生物技术通讯, 25(3): 316-322. (Huang Y, Yang M H, Bi B L, et al.2014.Cloning and expression analysis of FTO gene in Wujin pig[J].Biotechnology Communication, 25(3): 316-322.) [4] 林亚秋, 廖红海, 贺庆华, 等.2016.山羊FTO基因克隆及其表达谱[J].畜牧兽医学报, 47(5): 888-898. (Lin Y Q, Liao H H, He Q H, et al.2016.Cloning and expression profiling of goat FTO gene[J].Journal of Animal Husbandry and Veterinary Medicine, 47(5): 888-898.) [5] 王玉峰.2009.鸡FTO基因: 组织特异性表达、中枢核团定位、品种差异以及禁食的影响[D].硕士学位论文, 南京农业大学, 导师: 赵茹茜, pp.9-20. (Wang Y F.2009.Chicken FTO gene: Tissue-specific expression, central nucleus localization, variety differences and effects of fasting [D].Thesis for M.S., Nanjing Agricultural University, Supervisor: Zhao R Z, pp.9-20.) [6] 翁吉梅, 罗铮, 徐伟, 等.2018.黔北麻羊FTO基因多态性及生物信息学分析[J].中国畜牧杂志, 54(9): 60-64. (Weng J M, Luo Z, Xu W, et al.2018.Polymorphism and bioinformatics analysis of FTO gene in Qianbei Ma sheep[J].Chinese Journal of Animal Science, 54(9): 60-64.) [7] 吴森, 桂林生, 昝林森, 等.2018.南阳牛ATP5B基因启动子区域多态性与生长性状的关联性研究[J].农业生物技术学报, 26(9): 1535-1545. (Wu S, Gui L S, Zan L S, et al.2018.Correlation between polymorphisms of ATP5B gene promoter and growth traits in Nanyang cattle[J].Journal of Agricultural Biotechnology, 26(9): 1535-1545.) [8] 张莹, 吴铁梅, 王雪, 等.2016.脂肪与肥胖相关基因对动物脂肪代谢的调节[J].动物营养学报, 28(4): 999-1003. (Zhang Y, Wu T M, Wang X, et al.2016.Regulation of fat and obesity-related genes on fat metabolism in animals[J].Journal of Animal Nutrition, 28(4): 999-1003.) [9] 赵宪林, 曹少杰, 王俊全, 等.2018.MC4R基因多态性及基因型组合与藏羊生长性状的关联分析[J].农业生物技术学报, 26(3): 429-436. (Zhao X L, Cao S J, Wang J Q, et al.2018.Correlation analysis of polymorphism and genotype combination of MC4R gene and growth traits of Tibetan sheep[J].Journal of Agricultural Biotechnology, 26(3): 429-436.) [10] 朱琳娜.2014.FTO、METTL3基因表达对猪脂肪细胞mRNA N6-甲基腺苷水平及脂肪沉积的影响研究[D].浙江大学, 硕士学位论文, 导师: 汪以真, pp: 11-34. (Zhu L N.2014.Effects of FTO and METTL3 gene expression on N6-methyladenosine levels and fat deposition in porcine fat cells[D].Thesis for M.S., Zhejiang University, Suppervisor: Wang Y Z, pp: 11-34.) [11] Amélie B, Etienne L, Emmanuelle M, et al.2011.FTO is increased in muscle during type 2 diabetes, and its overexpression in myotubes alters insulin signaling, enhances lipogenesis and ROS production, and induces mitochondrial dysfunction[J].Diabetes, 60(1): 258-268. [12] Bravard A, Vial G, Chauvin M A, et al.2014.FTO contributes to hepatic metabolism regulation through regulation of leptin action and STAT3 signalling in liver[J].Cell Communication & Signaling, 12(1): 2-13. [13] Chen H, Pan Y X, Dudenhausen E E, et al.2004.Amino acid deprivation induces the transcription rate of the human asparagine synthetase gene through a timed program of expression and promoter binding of nutrient-responsive basic region/leucine zipper transcription factors as well as localized histone acetylation[J].Journal of Biological Chemistry, 279(49): 50829-50839. [14] Cheung M K, Gulati P, O'Rahilly S, et al.2013.FTO expression is regulated by availability of essential amino acids[J].International Journal of Obesity, 37(5): 744-747. [15] Falnes P Ø, Johansen R F, Seeberg E.2002.AlkB-mediated oxidative demethylation reverses DNA damage in Escherichia coli[J].Nature, 419(6903): 178-182. [16] Frayling T M, Timpson N J, Weedon M N, et al.2007.A common variant in the FTO gene is associated with Body Mass Index and predisposes to childhood and adult obesity[J].Science, 316(5826): 889-894. [17] Khella M S, Hamdy N M, Amin A I, et al.2017.The (FTO) gene polymorphism is associated with metabolic syndrome risk in Egyptian females: A case- control study[J].BMC Medical Genetics, 18(1): 101-108. [18] Mcmurray F, Church C D, Larder R, et al.2013.Adult onset global loss of the Fto gene alters body composition and metabolism in the mouse[J].PLoS Genetics, 9(1): e1003166. [19] Mo C, Yang M, Han X, et al.2017.Fat mass and obesity-associated protein attenuates lipid accumulation in macrophage foam cells and alleviates atherosclerosis in apolipoprotein E-deficient mice[J].Journal of Hypertension, 35(4): 810-821. [20] Palii S S, Kays C E, Deval C, et al.2009.Specificity of amino acid regulated gene expression: Analysis of genes subjected to either complete or single amino acid deprivation[J].Amino Acids, 37(1): 79-88. [21] Pawan G, Yeo G S H.2013.The biology of FTO: From nucleic acid demethylase to amino acid sensor[J].Diabetologia, 56(10): 2113-2121. [22] Roffeei S N, Mohamed Z, Reynolds G P, et al.2014.Association of FTO, LEPR and MTHFR gene polymorphisms with metabolic syndrome in schizophrenia patients receiving antipsychotics[J].Pharmacogenomics, 15(4): 477-485. [23] Sonestedt E, Gullberg B, Ericson U, et al.2011.Association between fat intake, physical activity and mortality depending on genetic variation in FTO[J].International Journal of Obesity, 35(8): 1041-1049. [24] Speakman J R.2010.FTO effect on energy demand versus food intake[J].Nature, 458(7289): 894-898. [25] Trewick S C, HenshawT F, Hausinger R P, et al.2002.Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage[J].Nature, 419(6903): 174-178. [26] Wåhlén K, Sjölin E, Hoffstedt J.2008.The common rs9939609 gene variant of the fat mass-and obesity-associated gene FTO is related to fat cell lipolysis[J].Journal of Lipid Research, 49(3): 607-611. [27] Zabena C, González-Sánchez J L, Martínez-Larrad M T, et al.2009.The FTO obesity gene.Genotyping and gene expression analysis in morbidly obese patients[J].Obesity Surgery, 19(1): 87-95.