Abstract：In this study, 11150 ESTs (including 10 cDNAs), about 8.1Mb in length, were downloaded from ESTs databases of Lentinula edodes in Fungal Genomics Project (FGP) website and NCBI website. By searching with SSRhunter 1.3 software and handwork, 469 SSRs were identified in 316 ESTs, approximately 2.83% of total ESTs. The average distance between SSRs was about 17.3kb. Trinucleotide and hexanucleotide ESR-SSRs were found to be the two most aboundent repeats, accounting for 38.00% and 20.00% of the total ESR-SSRs respectively, and (A)n, (T)n, (GA)n, (AG)n, (TGA)n, (GAT)n and (TCTTT)n to be the most familiar motifs, which comprised about 35.94% of all EST-SSRs. Agarose electrophoresis manifested that 39 out of 51 EST-SSRs primer pairs designed by Oligo 6.0 software possessed obvious PCR product in L. edodes, while there were no visible PCR product amplified by the selected primer pairs form other species. Some EST-SSR primer pairs revealed polymorphism in electrophorogram.
收稿日期: 2006-03-16
通讯作者:
林方灿
引用本文:
林范学 程水明 李安政 林方灿 . 香菇表达序列标签(EST)的SSR分析及EST-SSR引物的筛选[J]. , 2007, 15(2): 0-.
[英] LIN Fan-Xue [中] 林范学 Shui-Ming CHENG . Analysis of SSRs in ESTs database of Lentinula edodes and selection of EST-SSR primers. , 2007, 15(2): 0-.
