Abstract：To identify Hemagglutinating Encephalomyelitis Virus (HEV) 67N Receptor in PK Cells Membrance, express S1 protein of HEV in Pichia Pastoris, and purify the protein by Ni2+ affinity chromatograph. Polyclonal antibody to HEV was prepared with immunizing rabbits by injecting the purified S1 protein four times. After SDS-Polyacrylamid gel electrophoresis (SDS-PAGE), the PK cells membrance proteins were transferred to nitrocellulose membrance. VOPBA was performed by the recombinant S1 protein to identify the receptor protein binding HEV-S1. The result showed that HEV-S1protein bound one band about 90 kDa in PK cells membrance. This result is very important rationale to study thoroughly the pathogenic mechanism of Hemagglutinating Encephalomyelitis Virus.
