Abstract：Pluripotent stem cells (PSCs), which are regarded as important cell resources, have become the research focus around the world in recent years. Acquisition and acquaintance of these cells are of significance in medical research and biotechnology application. Pig(Sus scrofa) is considered to be the most important domestic animal in PSCs establishment, because of its similarities in physiology and metabolic to human(Homo sapiens). For now, however, pig PSCs establishment are still facing a lot of problems. Combined with the successful experiences of the PSCs research of mouse(Mus musculus) and human, we summarized cultural conditions, regulatory factors, signaling pathways, and gene expression profiling of those reported porcine PSCs. We provide useful information for the establishment of defined embryonic stem cells(ESCs) and iPSCs of pig.
