Abstract：Based on the consensus of the S-adenosylmethionine synthetase(SAM) and the sequence of the Bacillus thuringiensis genome, we designed a pair of primer for the S-adenosylmethionine synthetase gene of the Bacillus sphaericus.The fragment of S-adenosylmethionine synthetase gene of the Bacillus sphaericus was amplified by PCR. Sequence analysis was done after the fragment was inserted into the the expression vector pGEX-6P-1 to construct the plasmid pGEX-SAM. The results show that the homologous of the fragment with the SAM synthetase gene from E. coli, Bacillus subtilis, Saccharomyces cerevisiae, rat and Homo sapiens is 63.9%，75.4%，58.8%，59.1%，55.7% respectively. For the difference between the fragment and the reported SAM synthetase gene, we expressed the gene in E.coli DH5α and the purified 43KD proterin was acquired. The activity of catalyzing the formation of SAM was identified by determining the produced SAM through High Performance Liquid Chromatography.
收稿日期: 2006-06-08
通讯作者:
尹军团
引用本文:
尹军团 刘子铎 . 球形芽孢杆菌S-腺苷甲硫氨酸合成酶基因的克隆、表达与活性鉴定[J]. , 2007, 15(2): 0-.
. Cloning and Expression the S-adenosylmethionine synthetase from Bacillus sphaericus. , 2007, 15(2): 0-.
