Abstract:The genetic diversity and relationship of 10 P.ternta of JingZhou with different leaf shape were analyzed by SRAP. The DNAS of 10 P.ternta of JingZhou were tested with SRAP using 40 primer pairs and 39 primer pairs showed polymorphism. A total of 632 polymorphic bands were obtained and there were 16.2 polymorphic bands of per primer pairs. The SRAP based genetic similarity ranged 0.105~0.978, UPGMA cluster analysis based on SRAP molecular markers data divided 10 different leaf shape into five groups: the result of UPGMA divided trifoliate P.ternta of JingZhou and quinquefoliolate P.ternta of JingZhou into two group. There are four small group in trifoliate P.ternta of JingZhou:Ⅰ: auricular leaf、cuspate leaf、rounded leaf、terrace leaf、thin peony leaf and peony leaf;Ⅱ: auricular leaf with emargination;Ⅲ: wide angustifoliate;Ⅳ: angustifoliate. This study provided an new marker system for P.ternta variety identification and genetic research, which would be helped to reveal the genetic background and relationship of P.ternta germplasms .