Abstract：Experiments were conducted to determine the effect of lamb age, transport stimulation and repeated hormone superovulation on the number of collected oocytes, and to study the effect of the culture medium containing ethylene-diamine-tetra-acetic acid (EDTA) on the development of in vitro produced embryos from lamb oocytes. The results indicate that the mean number of collected and available oocytes from per 6- to 8- week lamb were 60.8±13.9 and 58.2±12.3 respectively, which were more than those (27.3±5.1 and 26.0±4.9 ) from per 12- to 14- week lamb(P<0.05); the transport stimulation didn’t decrease the number of collected oocytes from the superovulation lambs(P>0.05), however, the number of collected oocytes in the repeated superovulation group was reduced significantly compared with the control group(P<0.05); the embryonic culture medium containing 10 μmol EDTA could improved the development capability of in vitro produced embryo from lamb oocytes significantly(P<0.05), and the healthy lambs were born by the embryo transfer.
