Abstract：There is dramatic changing of histone modifications during in vitro maturating of oocytes, the changing of its expression pattern plays an important role on oocyte maturation and subsequently embryonic development. Suberoyl anilide hydroxamic acid (SAHA) acting as a anticancer drug is the potential inhibitor of histone deacetyltransferase, inhibiting the growth, differentiation and apoptosis of various types of tumor cell in vivo and vitro. To explore the effect of inhibitor of histone deacetyltransferase SAHA in vitro maturation and embryonic development potential of buffalo (Bubalus bubalus) oocytes, effects of maturing rate (first polar body discharge rate), cleavage rate of fertilized eggs, blastocyst rate and total cell number of blastocyst of buffalo oocytes were compared by different concentrations of (0, 2, 5, 10, 20 and 40 nmol/L) SAHA treatment. Results showed that maturing rate, cleavage rate, and blastocyst rate of group 10 nmol/LSAHA was significantly higher than control group ((77.07±1.79)% and (62.3±2.08)%, (86.81±1.93)% and (74.86±2.07)%, (27.56±2.86)% and (24.23±1.74)%, P＜0.05). As the concentration increasing, total cell number of blastocyst in each treatment group had a tendency to increase, but had no significantly difference with each other (141±10, 151±13, 165±17, 170±14, 147±20 and 185±22, P＞0.05). qRT-PCR tests revealed that the expression of (cAMP responsive element binding protein, CREB) binding protein gene (CBP), histone acetyltransferases 1(HAT1) gene and histonedeacetylase 1(HDAC1) gene in MⅡ stage oocytes of treatment groups were significantly reduced, though p300 was increased. In conclusion, histone deacetylase inhibitors SAHA was one of the most effective way to promote maturation of buffalo oocytes and embryonic development in vitro, this result will provid a theoretical basis for future study about gene regulatory mechanism of transgenic cloned embryonic-developmental.
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