猪孤雌激活胚胎的体外培养系统优化

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摘要胚胎体外培养的气相条件、培养基及生长因子是影响胚胎发育的关键因素。系统研究不同氧分压（7%O2和20%O2）和三种胚胎培养基(NCSU-23、 PZM-3、G3)及表皮生长因子(EGF)和碱性成纤维促进因子(bFGF）对孤雌激活胚胎的囊胚发育率及囊胚细胞数的影响。结果表明，与高氧(20%O2)条件相比，低氧条件下（7%O2）提高了胚胎的囊胚率和细胞数，有利于胚胎的发育。PZM-3能显著提高囊胚细胞数（P < 0.05，51.0±4.0 vs. 38.8±2.2），无论在高氧还是低氧下，PZM-3都是胚胎发育的一个效果稳定、高效的培养基。添加10ng/mL EGF或bFGF的NCSU-23对提高胚胎的囊胚发育率有显著效果（P < 0.05，36.2±5.3％，44.3±5.8％ vs. 20.7±6.3％），但不能提高囊胚细胞数（P > 0.05）。

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	潘登科冯书堂刘吉牟玉莲张莉

Abstract：Oxygen tension and culture media (CM) as well as growth factors are the key factors to the development of embryos in vitro. Systematically study had been carried on effect of oxygen tension（7% O2和20% O2）, culture media (NCSU-23、 PZM-3、G3) and growth factors(EGF, bFGF）on in vitro development of porcine parthenogenetically activated(PA) embryos, including blastocyst formation rate and cell number per blastocyst. Compared with 20%O2, low oxygen atmosphere improved blastocyst rate and total cell number; PA embryos in PZM-3 and 7 %O2 had a greater cell number (P < 0.05, 51.0±4.0 vs. 38.8±2.2). Regardless of atmosphere, PZM-3 supported a higher development rate and quality of porcine PA embryos than NCSU23 respectively. 2-4 cell stage PA embryos cultured in NCSU-23 supplemented with 10ng/mL EGF or bFGF had a significantly greater developmental ability to blastocyst than the control （P < 0.05, 36.2±5.3％，44.3±5.8％ vs. 20.7±6.3％）, no difference at blastocyst cell number between them.

收稿日期: 2006-11-20

通讯作者: 潘登科

引用本文:

潘登科冯书堂刘吉牟玉莲张莉. 猪孤雌激活胚胎的体外培养系统优化[J]. , 2007, 15(4): 0-.
. Optimization of in vitro culture system for the development of porcine parthenogenetic embryos. , 2007, 15(4): 0-.

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http://journal05.magtech.org.cn/Jwk_ny/CN/ 或 http://journal05.magtech.org.cn/Jwk_ny/CN/Y2007/V15/I4/0