Abstract：Oxygen tension and culture media (CM) as well as growth factors are the key factors to the development of embryos in vitro. Systematically study had been carried on effect of oxygen tension（7% O2和20% O2）, culture media (NCSU-23、 PZM-3、G3) and growth factors(EGF, bFGF）on in vitro development of porcine parthenogenetically activated(PA) embryos, including blastocyst formation rate and cell number per blastocyst. Compared with 20%O2, low oxygen atmosphere improved blastocyst rate and total cell number; PA embryos in PZM-3 and 7 %O2 had a greater cell number (P < 0.05, 51.0±4.0 vs. 38.8±2.2). Regardless of atmosphere, PZM-3 supported a higher development rate and quality of porcine PA embryos than NCSU23 respectively. 2-4 cell stage PA embryos cultured in NCSU-23 supplemented with 10ng/mL EGF or bFGF had a significantly greater developmental ability to blastocyst than the control （P < 0.05, 36.2±5.3％，44.3±5.8％ vs. 20.7±6.3％）, no difference at blastocyst cell number between them.
收稿日期: 2006-11-20
通讯作者:
潘登科
引用本文:
潘登科 冯书堂 刘 吉 牟玉莲 张 莉. 猪孤雌激活胚胎的体外培养系统优化[J]. , 2007, 15(4): 0-.
. Optimization of in vitro culture system for the development of porcine parthenogenetic embryos. , 2007, 15(4): 0-.
