Analysis of Genetic Characteristics of Cellulase in Potato Scab Pathogen in China
HUANG Yi-Fan1, DING Yu2, YANG Yun-Shan1, ZHANG Cai-Hua1, ZHAO Chuan-Xiang1, CHEN Chen1, HAN Zhi-Ruo1, YU Xiu-Mei1,3*, ZHAO Wei-Quan2*
1 College of Life Sciences/Key Laboratory of Hebei Province for Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding 071001, China; 2 College of Plant Protection/Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Hebei Agricultural University, Baoding 071001, China; 3 State Key Laboratory of North China Crop Improvement and Regulation, Baoding 071001, China
Abstract：Potato scab is a soil-borne disease caused by several scab-related Streptomyces spp., its' pathogenic mechanism remains unclear. Cellulase is reported as a key enzyme in many pathogens to infect the plant cell wall, the present study analyzed genetic characteristics of cellulase in the pathogenic Streptomyces spp. A total of 14 cellulases were identified from a typical potato common scab strain 87.22, although they had diversefunctional domain patterns, similar three-dimensional structures were detected in most of these cellulases. 7 pairs of gene specific primer were designed based on the sequence similarity of 14 cellulases, and used to amplify genomic DNA of 18 potato common scab strains and 5 potato scab pathogenic species collected fromdifferent potato (Solanum tuberosum) growing areas of China, it was found that 4 pairs of primers obtainedtarget bands in the 2 potato common scab strains No. MZ-4 and H2, and exhibited amplification polymorphism within the potato common scab strains as well as among potato scab pathogenic species, which indicated there was significant polymorphism in cellulases from different potato scab pathogens in China. Thisstudy provides a new support for in-depth analyzing the genetic diversity of the scab-related Streptomyces spp.
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