Polymorphism of GAB1 Gene in Hu Sheep (Ovis aries) and Its Association Analysis with Body Size Traits
ZHANG Jian1, WANG Wei-Min2, TIAN Hui-Bin2, ZHAO Li-Ming2, YANG Xiao-Bin2, MA Zong-Wu2, KONG De-Wen1, CAO Pei-Liang1, LI Hong-Jian1, LI Lin-Ting1, JIA Guo-Xing1, ZHANG Xiao-Xue1,*
1 College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China; 2 College of Grassland Agricultural Science and Technology, Lanzhou University, Lanzhou 730000, China
Abstract：Growth factor receptor-bound protein 2 (GRB2)-associated binding protein 1 (GAB1) is an adaptor protein that plays a role in the intracellular signaling cascade triggered by activated receptor type kinases. It plays an important role in maintaining the functional integrity of osteoblasts. The aim of this study was to analyze the association between single nucleotide polymorphisms (SNP) of GAB1 gene and body size traits in Hu sheep (Ovis aries). A total of 506 healthy Hu sheep with clear pedigree, accurate experimental data records and physical fitness were selected as the experimental subjects. Three genotypes (CC, TC and TT) of g.14514496 T>C polymorphism in exon 5 of GAB1 gene were detected by PCR amplification and AQPTM genotyping. The general linear model was used to analyze the association between body size traits and genotypes of sheep GAB1 gene. qPCR was used to detect the expression level of GAB1 gene in different tissues of Hu sheep. The result showed that sheep GAB1 gene was widely expressed in various tissues of Hu sheep, and its expression level was the highest in the rumen. The results of association analysis showed that this polymorphic locus was significantly associated with body height, body length and chest circumference of Hu sheep (P<0.05). For individuals carrying CC genotype, the body height of 140, 160 and 180 d of age, and the body length 160 and 180 d of age, and the chest circumference at 120 and 160 d of age were significantly higher than those of individuals carrying TT genotype (P<0.05), which suggested that GAB1 g.14514496 T>C might affect the body size traits of Hu sheep. Therefore, this locus can be used as a potential molecular marker. This study provides theoretical support for breeding studies of Hu sheep.
张健, 王维民, 田慧彬, 赵利明, 杨晓斌, 马宗武, 孔得文, 曹佩亮, 李泓舰, 李林庭, 贾国星, 张小雪. 湖羊GAB1基因多态性及其与体尺性状的关联分析[J]. 农业生物技术学报, 2025, 33(5): 1053-1062.
ZHANG Jian, WANG Wei-Min, TIAN Hui-Bin, ZHAO Li-Ming, YANG Xiao-Bin, MA Zong-Wu, KONG De-Wen, CAO Pei-Liang, LI Hong-Jian, LI Lin-Ting, JIA Guo-Xing, ZHANG Xiao-Xue. Polymorphism of GAB1 Gene in Hu Sheep (Ovis aries) and Its Association Analysis with Body Size Traits. 农业生物技术学报, 2025, 33(5): 1053-1062.
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