Expression Analysis of IGF-1, IGF-2 and TGF- β2 in the Lung of Yaks(Bos grunniens) in Different Ages
DENG Yan-Jiang1, ZHONG Dong-Lan1, CUI Yan1*, YU Si-Jiu1,2, WEI Peng-Qiang1, ZHANG Qian1, NIU Yue-Yue1, XIAN Kang-Le1, WANG Deng-Hui1, CHEN Chun-Yan1
1 College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2 Technology and Innovation Center of Gansu Province for Embryonic Engineering of livestock, Lanzhou 730070, China
Abstract：Insulin like growth factor (IGF) IGF-1 and IGF-2, as hypoxia target factors, can cooperate with transforming growth factor- β2 (TGF- β2) to participate in lung growth and development, immune regulation and injury repair. The purpose of this study was to investigate the expression and distribution characteristics of IGF-1, IGF-2 and TGF- β2 in the lungs of yaks (Bos grunniens) in different ages. The distribution and expression levels of IGF-1, IGF-2 and TGF- β2 in the lungs of yaks at different ages were investigated by immunohistochemical staining, qRT-PCR and Western blot. Immunohistochemical analysis showed that the expression positions of the three growth factors in the yak lungs were similar, and they were mainly distributed in all levels of bronchial epithelial cells and alveolar epithelial cells. In addition, IGF-1 and IGF-2were also expressed in pulmonary artery endothelial cells. The results of qRT-PCR showed that the expression levels of IGF-1 and TGF-β2 genes in the lungs of adult yaks were significantly higher than those of newborn, juvenile and elderly yaks (P<0.05). The expression of IGF-2 gene in the lung of newborn yak was significantly higher than that in other age groups (P<0.05). The results of Western blot showed that theexpression of IGF-1 and IGF-2 protein was the highest in the primary stage and significantly higher than that in other age groups (P<0.05). The expression of TGF- β2 protein was the highest in adulthood, and was significantly higher than that in other age groups (P<0.05). The expression levels of the 3 factors were similar with age, and the expression levels were higher in newborn and adulthood, suggesting that IGF-1, IGF-2, and TGF-β2 might be involved in the development of lung and the formation of hypoxic adaptive structure in yak at this age. This study provides the basis for exploring the specific adaptation mechanism of yak lung in hypoxic environment.
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